Abstract
The Method Of Hydroxyl Radical Detection Via The Conversion Of Terephthalate (Tpa) Into The Strongly Fluorescent Hydroxyterephthalate Has Been Used For A Long Time In Radiation Research. However, Attempts To Apply It In Biological Research Appeared Only Recently. Possibilities And Limitations Of The Tpa Method Were Investigated In Order To Adapt It For Chlorophyll-Containing Samples. Using Model Chemical Sources Of Various Reactive Oxygen Species We Confirmed That Tpa Very Sensitively Detects Hydroxyl Radicals, But Is Not Reactive To Either Hydrogen Peroxide Or Superoxide. Moreover, Using Rose Bengal As A Photosensitiser, We Showed That Tpa Is Also Insensitive To Singlet Oxygen. Here We Suggest The Optimal Conditions For Using The Method In Thylakoid Preparations And These Conditions Were Applied To Detect The Production Of Hydroxyl Radicals Induced By 365 Nm Uv Radiation. The High Selectivity Of Terephthalate Hydroxylation Towards Hydroxyl Radicals, The Relatively Low ToxiCity, Light-Insensitivity And Chemical Stability Of Both Probe And Its Hydroxylated Form Make This Simple Method Highly Promising For Application In In Vitro Studies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Armstrong WA, Facey RA, Grant DW, Humphreys WG (1963) A tissue-equivalent chemical dosimeter sensitive to 1 rad. Can J Chem 41:1575-1577.
Barreto JC, Smith GS, Strobel NHP, McQuillin PA, Miller TA (1995) Terephthalic acid: A dosimeter for the detection of hydroxyl radicals in vitro. Life Sci 56:PL 89-PL 96.
Fang XW, Mark G, von Sonntag C (1996) OH radical formation by ultrasound in aqueous solutions. Part I: The chemistry underlying the terephthalate dosimeter. Ultrason Sonochem 3:57-63.
Finkelstein E, Rosen GM, Rauckman EJ (1980) Spin trapping - kinetics of the reaction of superoxide and hydroxyl radicals with nitrones. J Am Chem Soc 102:4994-4999.
Li LX, Abe Y, Nagasawa Y, Kudo R, Usui N, Imai K, Mashino T, Mochizuki M, Miyata N (2004) An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. Biomed Chromatogr 18:470-474.
Matthews RM (1980) The radiation chemistry of the terephthalate dosimeter. Radiat Res 83:27-41.
Mishin VA, Thomas PE (2004) Characterization of hydroxyl radical formation by microsomal enzymes using a water-soluble trap, terephthalate. Biochem Pharmacol 68:747-752.
Qu XH, Kirschenbaum LJ, Borish ET (2000) Hydroxyterephthalate as a fluorescent probe for hydroxyl radicals: Application to hair melanin. Photochem Photobiol 71:307-313.
Tang B, Zhang L, Geng Y (2005) Determination of the antioxidant capacity of different food natural products with a new developed flow injection spectrofluorimetry detecting hydroxyl radicals. Talanta 65:769-775.
Yan EB, Unthank JK, Castillo-Melendez M, Miller SL, Langford SJ, Walker DW (2005) Novel method for in vivo hydroxyl radical measurement by microdialysis in fetal sheep brain in utero. J Appl Physiol 98:2304-2310.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer Science + Business Media, B.V.
About this paper
Cite this paper
Šnyrychová, I., Hideg, É. (2008). First Application of Terephthalate as a Fluorescent Probe for Hydroxyl Radicals in Thylakoid Membranes. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_333
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6709-9_333
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)