Abstract
Assaying functional Photosystem II (PSII) complexes by flash-induced oxygen yield from leaf tissue after photoinhibition is most direct but time-consuming, while measurement of chlorophyll fluorescence in leaves is only representative of chloroplasts near the leaf surface. To circumvent these deficiencies, we obtained an excellent linear correlation between (a) the integrated, transient electron flow (Σ) to PS I, after superimposing a single-turnover flash on steady far-red light, and (b) the relative oxygen yield per flash during progressive photoinactivation of Photosystem II. Leaves of C3 and C4 plants, woody and herbaceous species, wild type and a chlorophyll b-less mutant, and monocot and dicot plants gave a single linear correlation, which seems to be a universal relation for predicting the relative oxygen yield per flash from Σ.
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References
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Losciale, P., Oguchi, R., Hendrickson, L., Hope, A.B., Corelli-Grappadelli, L., Chow, W.S. (2008). A Universal Correlation Between Flash-Induced P700 Redox Kinetics and Photoinactivation of Photosystem II in All Leaves?. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_306
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_306
Publisher Name: Springer, Dordrecht
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Online ISBN: 978-1-4020-6709-9
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