Conversion of Chl a into Chl d by Heat-Treated Papain

  • Sohjiro Fukuyo
  • Shunsuke Ohashi
  • Koji Iwamoto
  • Yoshihiro Shiraiwa
  • Masami Kobayashi
Conference paper

Abstract

In 1943, Chl d was first reported as a minor pigment in several red macroalgae. In 1996, a novel cyanobacterium, Acaryochloris marina, was isolated from colonial ascidians, and A. marina was found to contain Chl d as the dominant chlorophyll. However, the biosynthesis pathway of Chl d has not been clarified yet. Chl d is thought to be synthesized from Chl a, like Chl b, while no experimental evidence was given. In 2004, we came across the formation of Chl d from Chl a in acetone or ethanol containing 10 % water in the presence of papain (EC 3.4.22.2) at room temperature in the dark. Chl d was not formed when papain was absent. Here we report the conversion of Chl a into Chl d in aqueous acetone at room temperature in the presence of papain treated at 96°C for 20 min. The yield of Chl d from Chl a did not change, when the heat-treated papain was used. The result indicate that conversion of Chl a into Chl d was not enzymatically caused by papain, but by something else contained in commercially available papain.

Keywords

Acaryochloris marina chlorophyll a chlorophyll d photodynamic therapy (PDT) photosensitizer photosynthesis 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Sohjiro Fukuyo
    • 1
  • Shunsuke Ohashi
    • 1
  • Koji Iwamoto
    • 2
  • Yoshihiro Shiraiwa
    • 2
  • Masami Kobayashi
    • 1
  1. 1.Institute of Materials ScienceUniversity of TsukubaTsukubaJapan
  2. 2.Institute of Biological SciencesUniversity of TsukubaTsukubaJapan

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