Abstract
The redox potential of the primary electron donor P700 of photosystem (PS) I has been determined for diverse organisms by spectroelectrochemistry with an optically transparent thin-layer electrochemical cell (OTTLE) to explore reasons for heavy scattering seen in the reported values of the P700 redox potential. The organisms submitted to examination were seven different organisms, four cyanobacteria including a primitive cyanobacteria Gloeobacter violaceus PCC7421, a red alga, a green algae and a higher plant. The P700 redox potential, determined within an error range of ±4 mV, showed a strong speciesdependence with a span over 70 mV. The causes for the strong species-dependence of the P700 redox potential are discussed in terms of the redox potentials of in vivo electron donors, cytochrome (cyt) c 6 and plastocyanin (PC).
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References
Cho YS, Wang QJ, Krogmann D, Whitmarsh J (1999) Extinction coefficients and midpoint potentials of cytochrome c6 from the cyanobacteria Arthrospira maxima, Microcystis aeruginosa, Synechocystis 6803. Biochim Biophys Acta 1413:92-97.
De la Rosa MA, Molina-Heredia FP, Hervás M, Navarro JA (2006) Convergent evolution of cytochrome c6 and plastocyanin. In: Golbeck JH (ed) Photosystem I: The Light-Driven Plastocyanin Ferredoxin Oxidoreductase, Springer, Dordrecht, The Netherlands, pp 683-696.
Díaz-Quintana A, Navarro JA, Hervás M, Molina-Heredia FP, De la Cerda B, De la Rosa MA (2003) A comparative structural and functional analysis of cyanobacterial plastocyanin and cytochrome c6 as alternative electron donors to photosystem I. Photsynth Res 75:97-110.
Jordan P, Fromme P, Witt HT, Klukas O, Saenger W, Krauß N (2001) Three dimensional structure of cyanobacterial photosystem I at 2.5 Å. Nature 411:909-917.
Ke B (2001) Photosynthesis: Photobiochemistry and Biophysics, Kluwer, Dordrecht, The Netherlands.
Ki HK (2005) Cytochrome c6 genes in cyanobacteria and higher plants. In: Pessarakli M (ed) Handbook of Photosynthesis, 2nd edn. CRC, Boca Raton, FL.
Nakamura A, Akai M, E Yoshida, Taki T, Watanabe T (2003) Reversed-phase HPLC determination of chlorophyll a’ and phylloquinone in photosystem I of oxygenic photosynthetic organisms. Eur J Biochem 270:2446-2458.
Nakamura A, Suzawa T, Watanabe T (2004) Spectroelectrochemical determination of the redox potential of P700 in spinach with an optically transparent thin-layer electrode. Chem Lett 33:688-689.
Nakamura A, Suzawa T, Kato Y, Watanabe T (2005) Significant species-dependence of P700 redox potential as verified by spectroelectrochemistry: Comparison of spinach and Thermosynechococcus elongatus. FEBS Lett 579:2273-2276.
Proux-Delrouyre V, Demaille C, Leibl W, Sétif P, Bottin H, Bourdillon C (2003) Electrocatalytic investigation of light-induced electron transfer between cytochrome c6 and photosystem I. J Am Chem Soc 125:13686-13692.
Sandmann G, Reck H, Kessler E, Böger P (1983) Distribution of plastocyanin and soluble plastidic cytochrome c in various classes of algae. Arch Microbiol 134:23-27
Watanabe T, Kobayashi M (1991) Electrochemistry of chlorophylls. In: Scheer H (ed) Chlorophylls. CRC, Boca Raton, FL, pp 287-303.
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Kato, Y., Nakamura, A., Suzawa, T., Watanabe, T. (2008). Unexpected Difference in the P700 Redox Potential Among Oxygenic Photosynthetic Organisms Revealed by Spectroelectrochemistry. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_25
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_25
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