The Function of MgDVP in a Chlorophyll d-Containing Organism

  • Martin Schliep
  • Min Chen
  • Anthony Larkum
  • Rosanne Quinnell
Conference paper

Abstract

The cyanobacterium Acaryochloris marina is an exceptional organism utilising chlorophyll d (Chl d) as its major photosynthetic pigment. Acaryochloris cells contain 90–99% Chl d with minor amounts of chlorophyll a and a chlorophyll c-like pigment. These unusual characteristics make it an excellent candidate to study various aspects of photosynthesis driven by Chl d. However, little is known about the pathway of Chl d biosynthesis. We specifically designed HPLC methods to analyse pigment compositions of Acaryochloris. This enabled us to detect intermediate products of the chlorophyll biosynthesis. We identified Mg-Protoporphyrin IX monomethyl ester (MgPMe) and Mg-2,4-divinyl pheoporphyrin (MgDVP) and the environmental factors influencing their concentration levels. HPLC-facilitated analysis of pigments from Acaryochloris cells cultured under various light quantities was performed; light stress conditions induced an increase in the ratio of MgDVP to Chl d. Pigment analysis of Acaryochloris cells grown under oxygen-stressed conditions demonstrated a decrease in MgDVP levels. We propose that the Chl d biosynthesis pathway favours an aerobic environment despite the fact that Acaryochloris cells can survive under anaerobic conditions.

Keywords

Chlorophyll d biosynthesis MgDVP HPLC Acaryochloris protoporphyrin protochlorophyllide 

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References246

  1. Bollivar DM (2006) Recent advances in chlorophyll biosynthesis. Photosynth Res 90:173-194.PubMedCrossRefGoogle Scholar
  2. Chen M, Bibby TS, Nield J, Larkum AWD, Barber J (2005) Structure of a large photosystem II supercomplex from Acaryochloris marina. FEBS Lett 579:1306-1310.PubMedCrossRefGoogle Scholar
  3. Helfrich M, Ross A, King GC, Turner AG, Larkum AWD (1999) Identification of [8-vinyl]-protochlorophyllide a in phototrophic prokaryotes and algae: Chemical and spectroscopic properties. Biochim Biophys Acta 1410:262-272.PubMedCrossRefGoogle Scholar
  4. Hu Q, Miyashita H, Iwasaki I, Kurano N, Miyachi S, Iwaki M, Itoh S (1998) A photosystem I reaction center driven by chlorophyll d in oxygenic photosynthesis. Proc Natl Acad Sci USA 95:13319-13323.PubMedCrossRefGoogle Scholar
  5. Jeffrey SW, Vesk M (1997) Introduction to marine phytoplankton and their pigment signatures. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods. UNESCO Publishing, Paris, pp 37-84.Google Scholar
  6. Jones DTG (1963) The production of magnesium protoporphyrin monomethyl ester by Rhodopseudomonas spheroides. Biochem J 86:429-432.PubMedGoogle Scholar
  7. Larkum AWD, Scaramuzzi C, Cox GC, Hiller RG, Turner AG (1994) Light-harvesting chlorophyll c-like pigment in Prochloron. Proc Natl Acad Sci USA 91:679-683.PubMedCrossRefGoogle Scholar
  8. Miyashita H, Adachi K, Kurano N, Ikemoto N, Chihara M, Miyachi S (1996) Chlorophyll d as a major pigment. Nature 338:402.CrossRefGoogle Scholar
  9. Miyashita H, Adachi K, Kurano N, Ikemoto H, Chihara M, Miyachi S (1997) Pigment composition of a novel oxygenic photosynthetic prokaryote containing chlorophyll d as the major chlorophyll. Plant Cell Physiol 38:274-281.CrossRefGoogle Scholar
  10. Pinta V, Picaud M, Reiss-Husson F, Astier C (2002) Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-clustercontaining protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester. J Bacteriol 184:746-753.PubMedCrossRefGoogle Scholar
  11. Tomo T, Okubo T, Akimoto S, Yokono M, Miyashita H, Tsuchiya T, Noguchi T, Mimuro M (2007) Identification of the special pair of photosystem II in a chlorophyll d-dominated cyanobacterium. Proc Natl Acad Sci USA 104:7283-7288.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Martin Schliep
    • 1
  • Min Chen
    • 1
  • Anthony Larkum
    • 1
  • Rosanne Quinnell
    • 1
  1. 1.School of Biological SciencesThe University of SydneySydneyAustralia

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