Abstract
TSP9 is the thylakoid soluble phosphoprotein of 9 kDa, an intrinsically unstructured plant specific protein that has earlier been found interacting with light harvesting proteins and both photosystems in photosynthetic thylakoid membranes from spinach. We investigated the function of this protein in Arabidopsis thaliana using reverse genetics, biochemical and fluorometric methods.\ Recombinant TSP9 was used as a substrate for light-induced phosphorylation by thylakoid membranes isolated from wild type and two mutant lines lacking either STN7 or STN8 protein kinases. TSP9 was found phosphorylated by STN7 kinase. Phosphorylation of light-harvesting complex II was found reduced in knockout plants incapable of expressing TSP9. Chlorophyll fluorescence studies showed reduced ability of the TSP9 lacking mutant to perform the photosynthetic state transitions, as well as decreased non-photochemical quenching as compared with the wild type plants. We propose a\ role for this small soluble protein in the regulation of light harvesting in Arabidopsis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Allen JF (1992) Protein phosphorylation in regulation of photosynthesis. Biochim Biophys Acta 1098:275-335.
Allen JF (2003) State transitions--a question of balance. Science 299:1530-1532.
Bellafiore S, Barneche F, Peltier G, Rochaix JD (2005) State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature 433:892-895.
Carlberg I, Hansson M, Kieselbach T, Schroder WP, Andersson B, Vener AV (2003) A novel plant protein undergoing light-induced phosphorylation and release from the photosynthetic thylakoid membranes. Proc Natl Acad Sci USA 100:757-762.
Depege N, Bellafiore S, Rochaix JD (2003) Role of chloroplast protein Stt7 kinase in LHCII phosphorylation and state transition in Chlamydomonas. Science 299:1572-1575.
Haldrup A, Naver H, Scheller HV (1999) The interaction between plastocyanin and photosystem I is inefficient in transgenic Arabidopsis plants lacking the PSI-N subunit of photosystem I. Plant J 17:689-698.
Haldrup A, Jensen P E, Lunde C, Scheller HV (2001) Balance of power: A view of the mechanism of photosynthetic state transitions. Trends Plant Sci 6:301-305.
Hansson M, Dupuis T, Stromquist R, Andersson B, Vener AV, Carlberg I (2007) The Mobile Thylakoid Phosphoprotein TSP9 Interacts with the Light-harvesting Complex II and the Peripheries of Both Photosystems. J Biol Chem 282:16214-16222.
Kieselbach T, Hagman Å, Andersson B, Schröder WP (1998) The thylakoid lumen of chloroplats, isolation and characterization. J Biol Chem 273:6710-6716.
Li XP, Bjorkman O. Shih,C, Grossman AR, Rosenquist M, Jansson S, Niyogi KK (2000) A pigment-binding protein essential for regulation of photosynthetic light harvesting. Nature 403:391-395.
Rochaix JD (2007) Role of thylakoid protein kinases in photosynthetic acclimation. FEBS Lett 581:2768-2775.
Vainonen J P, Hansson M, Vener AV (2005) STN8 Protein Kinase in Arabidopsis thaliana is Specific in Phosphorylation of Photosystem II Core Proteins. J Biol Chem 280:33679-33686.
Vener AV (2007) Biochim Biophys Acta 1767, 449-457.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer Science + Business Media, B.V.
About this paper
Cite this paper
Fristedt, R., Carlberg, I., Zygadlo, A., Scheller, H.V., Vener, A.V. (2008). Involvement of TSP9 Phosphoprotein in Balancing the Photosynthetic Light Harvesting Process in Arabidopsis thaliana . In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_220
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6709-9_220
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)