Abstract
The purified photosystem I (PS I) reaction center complex of a cyanobacterium Acaryochloris marina contained 88 Chl d: 1.1 Chl a: 19 carotenoids: 2.0 phylloquinone. Amino acid sequences of PsaA and PsaB polypeptides were almost homologous to those in the other cyanobacteria. The ligands for A0 was Met698A and that for A0′ was Leu688B but not Met that is conserved in all the other PS I. Laser excitation induced the 10-ps bleach and the 40- ps recovery at 680 nm of Chl a-type pigment in parallel with the 49-ps bleach of the Chl d-dimer P740 at 740 nm. The results indicate that A0 is Chl a-680 ligated by Met698A and that the PsaB branch with Leu688B is inactive for the electron transfer. The PS I of A. marina, thus, is the unique asymmetric type I reaction center with the unidirectional electron transfer pathway only through PsaA branch.
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References
Fairclough WV, Forsyth A, Evans MCW, Rigby SEJ, Purton S, Heathcote P (2003) Bidirectional electron transfer in photosystem I: Electron transfer on the PsaA side is not essential for phototrophic growth in Chlamydomonas. Biochim Biophys Acta 1606:43-55.
Hu Q, Miyashita H, Iwasaki I, Kurano N, Miyachi S, Iwaki M, Itoh S (1998) A photosystem I reaction center driven by chlorophyll d in oxygenic photosynthesis. Proc Natl Acad Sci USA 95:13319-13323.
Itoh S, Iwaki M, Ikegami I (2001) Modification of photosystem I reaction center by the extraction and exchange of chlorophylls and quinones. Biochim Biophys Acta 1507:115-138.
Jordan P, Fromme P, Witt HT, Klukas O, Saenger W, Krauss N (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5 angstrom resolution. Nature 411:909-917.
Kumazaki S, Abiko K, Ikegami I, Iwaki M, Itoh S (2002) Energy equilibration and primary charge separation in chlorophyll d-based photosystem I reaction center isolated from A. marina. FEBS Lett 530:153-157.
Miyashita H, Ikemoto H, Kurano N, Adachi K, Chihara M, Miyachi S (1996) Chlorophyll d as a major pigment. Nature 383:402.
Takaichi S, Shimada K (1992) Characterization of carotenoids in photosynthetic bacteria. Methods Enzymol 213:374-385.
Xu W, Chitnis P, Valieva A, van der Est A, Pushkar YN, Krzystyniak M, Teutloff C, Zech S G, Bittl R, Stehlik D, Zybailov B, Shen G, Golbeck JH (2003) Electron transfer in cyanobacterial photosystem I. J Biol Chem 278:27864-27875.
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Itoh, S. et al. (2008). Unidirectional Electron Transfer in Chlorophyll d-Containing Photosystem I Reaction Center Complex of Acaryochloris marina . In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_21
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_21
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