Purified Heterocysts from Nostoc punctiforme Studied by Laser Scanning Confocal Microscopy

  • Tanai Cardona
  • Stenbjörn Styring
  • Peter Lindblad
  • Ann Magnuson
Conference paper

Abstract

Few Detailed Investigations Exist On The Thylakoid Membranes Of Nitrogen-Fixing Multicellular Cyanobacteria; Especially The Changes That Occur During Cell Differentiation And The Possible Functional Differences That Arise As A Consequence Of Nitrogen Fixation. Protocols For The Purification Of Thylakoid Membranes, From Vegetative Cells And Isolated Heterocysts From Nostoc Punctiforme Sp. Strain Pcc 73102 Have Been Developed To Investigate Their Biochemical And Biophysical Properties. Laser Scanning Confocal Microscopy Was Implemented To Estimate The Purity And Quality Of The Heterocyst Preparations Using The Stronger Emission Of Vegetative Cells As A Marker When Excited With Laser Light At 488 Nm.

Keywords

Heterocyst confocal microscopy Nostoc 

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References

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Tanai Cardona
    • 1
  • Stenbjörn Styring
    • 1
  • Peter Lindblad
    • 1
  • Ann Magnuson
    • 1
  1. 1.Department of Photochemistry and Molecular ScienceUppsala UniversityUppsalaSweden

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