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Investigation of the Function of a nuoE Homologue, sll1220 in Synechocystis sp. PCC6803 by Means of Mutagenesis

  • Hiroyuki Koike
  • Md Rafiqul Islam
  • Kazuhiko Satoh
Conference paper

Abstract

Diatoms play a key role in an aquatic ecosystem on the earth as the primary photosynthetic producers. We developed a new method to purify PS I complexes from Chaetoceros gracilis and Thalassiosira pseudonana. The molecular amount of chlorophyll (Chl) per P700 was 255 in the purified PS I complex from C. gracilis. While the Chl/P700 ratio of thylakoid membranes from C. gracilis was 1,346. High-performance liquid chromatography (HPLC) showed that the PS I complexes contained 30.6 Chl c, 69.7 fucoxanthin, 34.2 diadinoxanthin and diatoxanthin, 1.0 violaxantin, 20.1 B-carotene and 2.1 menaquinone-4 molecules per 255 Chl a. Rates of electron transfer in the purified PS I complexes from C. gracilis were mesured directly. The electron transfer rate of the purified PS I complexes was 1.85 × 105 μmol mg−1 Chl a h−1 with cytochrome c6 from C. gracilis and methyl viologen (MV) as an electron donor and acceptor, respectively. Purified PS I complexes showed '100 times higher oxidation rate than thylakoid membranes.

Keywords

Photosystem I (PS I) diatom fucoxanthin-chlorophyll binding protein (FCP) antenna size 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Hiroyuki Koike
    • 1
  • Md Rafiqul Islam
    • 1
  • Kazuhiko Satoh
    • 1
  1. 1.Graduate School of Life Science, Faculty of ScienceUniversity of HyogoHarima Science Garden cityJapan

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