Abstract
Photosynthesis is one of the most important determinants of crop productivity. Although many studies have been conducted to improve yield, very little progress has been made. In this study, we used rice FOX (Full length cDNA over-expressing) lines that rice full-length cDNAs were over-expressed in Arabidopsis to identify novel photosynthesis-related genes of rice. We have used imaging of chlorophyll fluorescence to screen candidates. To confirm over-expression of transformed cDNAs cause the observed phenotype, we generated transformants overexpressing each cDNA, resulting that eight lines showed the same phenotype as original lines. Isolated candidates could be classified into four categories depending on chlorophyll fluorescence parameters. We analyzed gene expression profile of three candidate lines showing similar photosynthetic characteristics using DNA microarray. We found commonly regulated genes among three candidates, indicating that candidates have both shared and independent influences on gene expression.
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References
Bosco CD, Lezhneva L, Biehl A, Leister D, Strotmann H, Wanner G, Meurer J (2004) Inactivation of the chloroplast ATP synthase γ subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. J Biol Chem 279 (2):1060-1069.
Goff SA, Ricke D, Lan TH, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange BM, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun WL, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller RM, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296 (5565):92-100.
Haldrup A, Lunde C, Scheller HV (2003) Arabidopsis thal-iana plants lacking the PSI-D subunit of photosystem I suffer severe photoinhibition, have unstable photosystem I complexes, and altered redox homeostasis in the chlo-roplast stroma. J Biol Chem 278 (35):33276-33283.
Ichikawa T, Nakazawa M, Kawashima M, Iizumi H, Kuroda H, Kondou Y, Tsuhara Y, Suzuki K, Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K, Matsui M (2006) The FOX hunting system: An alternative gain-of-function gene hunting technique. Plant J 48:974-985.
Rice Full-Length cDNA Consortium; National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team, Foundation of Advancement of International Science Genome Sequencing & Analysis Group, RIKEN (2003) Collection, mapping, and anno-tation of over 28,000 cDNA clones from japonica rice. Science 301 (5631):376-379.
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Higuchi, M. et al. (2008). Identification of Photosynthesis-Related Genes in Rice Using FOX Hunting System. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_134
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_134
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
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