Abstract
The electronic structures of the cofactors involved into the primary radical pairs in LH1-bound RCs of Rhodopseudomonas acidophila and in isolated RCs of Rhodobacter sphaeroides are compared by photochemically induced dynamic nuclear polariziation (photo-CIDNP) magic-angle spinning (MAS) NMR. Only minor differences in the electronic ground-state of the donor and a very similar distribution of the electron spin density in the radical pair state is observed. On the other hand, the ratio between donor and acceptor signals appears to be slightly different. The origin of the effect is discussed.
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Alia, Matysik J, Soede-Huijbregts C, Baldus M, Raap J, Lugtenburg J, Gast P, van Gorkom HJ, Hoff AJ, de Groot HJM (2001) Ultra high field MAS NMR dipolar correlation spectroscopy of histidine residues in light harvesting complex II from photosynthetic bacteria reveals partial internal charge transfer in the B850/his complex. J Am Chem Soc 123:4803-4809.
Alia, Roy E, Gast P, van Gorkom HJ, de Groot HJM, Jeschke G, Matysik J (2004) Photochemically induced dynamic nuclear polarisation in photosystem I of plants observed by 13C magic-angle spinning NMR. J Am Chem Soc 126:12819-12826.
Groot HJM (2000) Photochemically induced dynamic nuclear polarization in reaction centres of photosystem II observed by 13C-solid-state NMR reveals a strongly asymmetric electronic structure of the P680•+primary donor chlorophyll. Proc Natl Acad Sci USA 97:9865-9870.
Prakash S, Alia, Gast P, de Groot HJM, Jeschke G, Matysik J (2005) Magnetic field dependence of photo-CIDNP MAS NMR on photosynthetic reaction centres of Rhodobacter sphaeroides WT. J Am Chem Soc 127:14290-14298.
Prakash S, Alia, Gast P, de Groot HJM, Matysik J, Jeschke G (2006) Photo-CIDNP MAS NMR in intact cells of Rhodobacter sphaeroides R26: Molecular and atomic resolution at nanomolar concentration. J Am Chem Soc 128:12794-12799.
Raap J, Winkel C, de Wit AHM, van Houten AHH, Hoff AJ, Lugtenburg J (1990) Mass spectrometric determination of isotopically labeled tyrosines and tryptophans in photosynthetic reaction centers of Rhodobacter sphaeroides R-26. Anal Biochem 191:9-15.
Roy E, Alia, Gast P, van Gorkom HJ, de Groot HJM, Jeschke G, Matysik J (2007) Photochemically induced dynamic nuclear polarisation observed in the reaction center of the green sulphur bacteria Chlorobium tepidum by 13C MAS NMR. Biochem Biophys Acta 1767:610-615.
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Diller, A., Alia, A., Gast, P., Jeschke, G., Matysik, J. (2008). 13C Photo-CIDNP MAS NMR on the LH1-RC Complex of Rhodopseudomonas acidophila . In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_13
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
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