Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza Virus Confers Resistance to the Virus in Transgenic Mexican Lime

  • Carmen Fagoaga
  • Carmelo López
  • Alfonso Hermoso de Mendoza
  • Pedro Moreno
  • Luis Navarro
  • Ricardo Flores
  • Leandro Peña
Conference paper

Citrus is the most important fruit tree crop in the world, with a cultivated surface of more than 7 million ha distributed in about 100 countries, mainly in tropical and subtropical areas, and a fruit production of more than 100 million tons per year (FAO, 2005). Citrus tristeza virus (CTV) causes the most destructive viral disease and is considered a major threat for the citrus industry worldwide. CTV produces decline and death of scion varieties, except lemons (Citrus limon (L.) Burm.), grafted on sour orange (C. aurantium (L.). Additionally, severe CTV strains cause stem pitting, stunting, low yield and poor fruit quality of some varieties of sweet orange (C. sinensis (L.) Osb.), limes (C. aurantifolia (Christ.) Swing.; C. latifolia Tan.) and grapefruits (C. paradisi Macf.), regardless the rootstock used (Bar-Joseph et al., 1989).

Keywords

Lime Microbe 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Bar-Joseph M, Marcus R, Lee RF (1989) The continuous challenge of citrus tristeza virus control. Annu Rev Phytopathol 27: 291-316.CrossRefGoogle Scholar
  2. Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña, L (2005) Viral-like symptoms induced by the ectopic expression of the p23 gene of citrus tristeza virus are citrus-specific and do not correlate with the pathogenicity of the virus strain. Mol. Plant Microbe Interaction 18: 435-445. FAO (2005) http//faostat.fao.org.
  3. Ghorbel R, López C, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L (2001) Transgenic citrus plants expressing the citrus tristeza virus p23 protein exhibit viral-like symptoms. Mol Plant Pathol 2: 27-36.CrossRefGoogle Scholar
  4. Karasev AV, Boyko VP, Gowda S, Nikolaeva OV, Hilf ME, Koonin EV, Niblett CL, Cline K, Gumpf DJ, Lee RF, Garnsey SM, Lewandowsky DJ, Dawson WO (1995) Complete sequence of the citrus tristeza virus RNA genome. Virology 208: 511-520.CrossRefPubMedGoogle Scholar
  5. López C, Navas-Castillo J, Gowda S, Moreno P, Flores R (2000) The 23 kDa protein coded by the 3’-terminal gene of citrus tristeza virus is an RNA-binding protein. Virology 269: 462-470.CrossRefPubMedGoogle Scholar
  6. Lu R, Folimonov A, Shintaku M, Li W-X, Falk BW, Dawson WO, Ding SW (2004). Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Nat Acad Sci USA 101: 15742-15747.CrossRefPubMedGoogle Scholar
  7. Satyanarayana T, Gowda S, Ayllón MA, Albiach-Martí MR, Rabindran S, Dawson WO (2002). The p23 protein of citrus tristeza virus controls asym-metrical RNA accumulation. J Virol 76: 473-483.CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Carmen Fagoaga
    • 1
  • Carmelo López
    • 2
  • Alfonso Hermoso de Mendoza
    • 1
  • Pedro Moreno
    • 1
  • Luis Navarro
    • 1
  • Ricardo Flores
    • 2
  • Leandro Peña
    • 2
  1. 1.Instituto Valenciano de Investigaciones AgrariasMoncadaSpain
  2. 2.Instituto de Biología Molecular y Celular de PlantasUniversidad Politécnica de ValenciaSpain

Personalised recommendations