In Vitro Testing of Inhalable Fly Ash at the Air Liquid Interface

  • Sonja Mülhopt
  • Hanns-Rudolf Paur
  • Silvia Diabaté
  • Harald F. Krug

The aim of this study is to analyse the toxicological potential of fine and ultrafine particles from industrial combustion processes using a biotest. This biotest is performed by near-realistic exposure of cultivated lung cells at the air-liquid interface and analysing the biological responses. Important steps in this work are to develop the exposure system for the use at industrial particle sources, to provide reproducible deposition conditions for submicron particles and to validate the exposure protocol for the bioassay. The presented technique maintains the viability of the cells but is sensitive for inflammatory effects. Exposure experiments with the ultrafine fraction of fly ash from a municipal waste incinerator have shown an increased release of IL-8 as a function of exposure time and dose. The presented exposure method and the lung specific bioassay seem to be an appropriate model to simulate the inhalation of particulate air pollution and to screen the biological effects of particulate emissions from different sources.

Keywords: Aerosol, bioassay, PM10, toxicity, ultrafine particles


Exposure System Ultrafine Particle Diesel Exhaust Particle Exposure Chamber Normal Human Bronchial Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer 2008

Authors and Affiliations

  • Sonja Mülhopt
    • 1
  • Hanns-Rudolf Paur
    • 1
  • Silvia Diabaté
    • 2
  • Harald F. Krug
    • 2
  1. 1.Thermal Waste Treatment DivisionForschungszentrum Karlsruhe, Institute for Technical ChemistryGermany
  2. 2.Forschungszentrum KarlsruheInstitute for Toxicology and GeneticsGermany

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