Light-Emitting Diodes as an Effective Lighting Source for In Vitro Banana Culture

  • D. T. Nhut
  • N. T. Don
  • M. Tanaka

This chapter describes a procedure using LEDs as a lighting source for banana plant tissue culture as a case study. With further improvements depending on specific plant species, the whole irradiation system using super bright red and blue LEDs could become a major light source for a wide range of plant culture systems.


Blue Lead Root Fresh Weight Shoot Fresh Weight Rock Wool Plantlet Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bula, R.J., Morrow, T.W., Tibbitts, T.W., Barta, D.J., Ignatius, R.W. & Martin, T.S. (1991) Lightemitting diodes as a radiation source for plants. HortSci. 26, 203-205.Google Scholar
  2. Eels, J.T., Henry, M.M., Summerfelt, P., Wong-Riley, M.T.T., Buchmann, E.V., Kane, M., Whelan, N.T. & Whelan, H.T. (2003) Therapeutic photobiomodulation for methanol-induced retinal toxicity. PNAS 100, 3439-3444.CrossRefGoogle Scholar
  3. Hoenecke, M.E., Bula, R.J. & Tibbitts, T.W. (1992) Importance of “blue” photon levels for lettuce seedlings grow under red light-emitting diodes. HortSci. 27, 427-430.Google Scholar
  4. Lee, C.G. & Palsson, B.O. (1994) High-density algal photobioreactors using light-emitting diodes. Biotech. Bioeng. 44, 1161-1167.CrossRefGoogle Scholar
  5. Murashige, T. & Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.CrossRefGoogle Scholar
  6. Nhut, D.T. (2002) In vitro growth and physiological aspects of some horticultural plantlets cultured under red and blue light-emitting diodes (LEDs). PhD Thesis, Kagawa Univ., Japan.Google Scholar
  7. Nhut, D.T. (2005) Light-emitting diodes (LEDs): an artificial flexible lighting source for biological studies. 1st Intl. Dev. Med. Eng., Viet Nam, pp. 109-113.Google Scholar
  8. Nhut, D.T., Hong, L.T.A., Watanabe, H., Goi, M. & Tanaka, M. (2001) In vitro growth of banana plantlets cultured under red and blue light-emitting diodes (LEDs) irradiation source. Acta Hort. 575, 117-124.Google Scholar
  9. Nhut, D.T., Takamura, T., Watanabe, H. & Tanaka, M. (2003) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent of micropropagated banana. Acta Hort. 616, 121-128.Google Scholar
  10. Nhut, D.T., Thuy, D.T.T., Luan, V.Q., Don, N.T., Khiem, D.V., Tran Thanh Van, K. (2005) Micropropagation of Paphiopedilum delenatii via stem node culture. Viet Nam-Korea Intl. Sym. 2005 Bio-Tech. & Bio-Sys. Eng., Nong Lam Univ., Viet Nam, pp. 184-189.Google Scholar
  11. Nhut, D.T., Thuy, D.T.T., Don, N.T., Luan, V.Q., Hai, N.T., Tran Thanh Van, K. & Chinnappa, C.C. (2007) In vitro stem elongation of Paphiopedilum delenatii Guillaumin and shoot regeneration via stem node culture. Prop. Orn. Plants 7 (1), 29-36.Google Scholar
  12. Okamoto, K., Yanagi, T., Takita, S., Tanaka, M., Higuchi, T., Ushida, Y. & Watanabe, T. (1996) Development of plant growth apparatus using blue and red LED as artificial light source. Acta Hort. 440, 111-116.Google Scholar
  13. Robin, C., Hay, M.J.M. & Newton, P.C.D. (1994a) Effect of light quality (red:far-red ratio) and defoliation treatments applied at a single phytomer on axillary bud outgrowth in Trifolium repens L. Oecol. 100, 236-242.CrossRefGoogle Scholar
  14. Robin, C., Hay, M.J.M., Newton, P.C.D. & Greer, D.H. (1994b) Effect of light quality (red:far-red ratio) at the apical bud of the main stolon on morphogenesis of Trifolium repens L. Ann. Bot. 72, 119-123.CrossRefGoogle Scholar
  15. Robinson, J.C. (1996) Banana and plantains. CAB Intl., Univ. Press, Cambridge, UK, pp. 104-127.Google Scholar
  16. Schmidt, M.H., Meyer, G.A., Reichert, K.W., Cheng, J., Krouwer, H.G., Ozker, K. & Whelan, H.T. (2004) Evaluation of photodynamic therapy near functional brain tissue in patients with recurrent brain tumours. J. Neuro-Oncol. 67, 201-207.CrossRefGoogle Scholar
  17. Tanaka, M., Goi, M. & Higashiura, T. (1988) A novel disposable culture vessel made of fluorocarbon polymer films for micropropagation. Acta Hort. 226, 663-670.Google Scholar
  18. Tanaka, M., Hirano, T., Goi, M., Higashiura, T., Sasahara, H. & Murasaki, K. (1991) Practical application of a novel disposable film culture vessel in micropropagation. Acta Hort. 300, 77-84.Google Scholar
  19. Tanaka, M., Nhut, D.T., Takamura, T., Watanabe, H. & Okamoto, K. (1998) In vitro growth of strawberry plantlets cultured under superbright red and blue light-emitting diodes (LEDs). XXVth Intl. Hort. Congr., Belgium, pp. 407.Google Scholar
  20. Tennessen, D.J., Singsaas, E.L. & Sharkey, T.D. (1994) Light-emitting diodes as a light source for photosynthesis research. Photosyn. Res. 39, 85-92.CrossRefGoogle Scholar
  21. Tripathy, B.C. & Brown, C.S. (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol. 107, 407-411.PubMedGoogle Scholar
  22. Whelan, H.T., Buchmannla, E.V., Whelan, N.T., Turnela, S.G., Cevenini, V., Stinson, H., Ignatius, R., Martin, T., Cwiklinski, J., Meyeric, G.A., Hodgson, B., Gouldl, L., Kane, M., Chen, G. & Caviness, J. (2001) NASA light-emitting diode medical applications from deep space to deep sea. In: El-Genk M.S. (Ed.) Space Technology and Applications International Forum 2001, pp. 35-45.Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • D. T. Nhut
    • 1
  • N. T. Don
    • 1
  • M. Tanaka
    • 2
  1. 1.Da Lat Institute of BiologyLam DongViet Nam
  2. 2.Faculty of AgricultureKagawa UniversityMiki-choJapan

Personalised recommendations