Advertisement

Clastogenic Factors, Bystander Effects and Genomic Instability In Vivo

Conference paper
Part of the NATO Science for Peace and Security Series book series (NAPSC)

For the last 15 years, we have investigated low dose radiation genetic effects on human populations affected by the Chernobyl accident. Cytogenetic longitudinal investigations showed that amount of radiation markers for clean-up workers remained at the elevated level and had trend to grow up with the time. A dynamic profile of the amount of aberrations confirms that this group has symptoms of the genomic instability. State of the genomic instability correlates with accumulation of clastogenic factors, responsible for increased genomic instability in clean-up workers peripheral blood. As a model for clastogenic activity testing, we used human keratinocyte cell line with blocked 1st check point of cell cycle. Our results confirm that cytogenetic and molecular effects of irradiation can be fixed even 20 years after the Chernobyl accident.

Keywords

Genomic Instability Bystander Effect Chernobyl Accident Aberration Frequency Acute Virus Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akaike, Takaaki, 2001, Role of free radicals in viral pathogenesis and mutation, Rev Med Virol 11(2):87–101.CrossRefGoogle Scholar
  2. Kash, J.C., Basler, C.F., Garcia-Sastre, A., Carter, V., Billharz, R., Swayne, D.E., Przygodzki, R.M., Taubennerger, J.K., Katze, M.G., and Tumpey, T.M., 2004, Global host immune response: pathogenesis and transcriptional profiling of type A influenza viruses expressing the hemagglutinin and neuraminidase genes from the 1918 pandemic virus, J Virol 78 (17):9499–511.CrossRefGoogle Scholar
  3. Marozik, P., Mothersill, C., Seymour, C.B., Mosse, I., and Melnov, S., 2007, Bystander effect induced by serum from the survivors of the Chernobyl accident, Exp Haem, 35:55–63.CrossRefGoogle Scholar
  4. Melnov, S.B., 2004, Molecular and Genetic Effects of Ecological Trouble (Possibilities of Flow Cytometry), Committee “Chernobyl children”, Minsk, pp. 10–32.Google Scholar
  5. Mothersill, C. and Seymour, C., 1997, Medium from irradiated human epithelial cells but not human fibroblasts reduces the clonogenic survival of unirradiated cells, Int J Radiat Biol 71:421–427.CrossRefGoogle Scholar
  6. Nagasawa, H. and Little, J.B., 1992, Induction of sister chromatid exchanges by extremely low doses of alpha-particles, Cancer Res 52:6394–396.Google Scholar
  7. Swaun, S.D., Wright, T.W., Degel, P.M., et al., 2004, Neither neutrophils nor reactive oxygen species contribute to tissue damage during Pheumocystis pneumonia in mice, Infect Immun 72(10):5722–732.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  1. 1.Department of Environmental and Molecular GeneticsInternational Sakharov Environmental UniversityBelarus
  2. 2.Institute of Genetics and Cytology National Academy of Sciences of BelarusBelarus

Personalised recommendations