Lentil pp 225-240 | Cite as

Wild Relatives And Biotechnological Approaches



Wild species of the genus Lens are an important source of genetic variation for breeding lentil varieties adaptable to new environments and tolerant of biotic and abiotic stresses. The wild species are endemic to a wide range of environments and possess many diverse characteristics. Lens species can be divided into three groups, a primary, secondary and tertiary gene pool, according to their inter-crossability. Crosses between members of the different genepools generally fail because the hybrid embryos abort. However, embryo rescue has been used successfully to obtain viable hybrids between groups. It is possible to intercross most of the wild Lens species with cultivated lentils using plant growth regulators and/or embryo rescue to allow the growth of hybrid plants. Other biotechnology techniques which may impact on lentil breeding include, micropropagation using meristamatic explants, callus culture and regeneration, protoplast culture and doubled haploid production. Micropropagation and regeneration from callus culture are relatively well established techniques with further research required for the development of reliable protoplast regeneration and doubled haploid protocols


Ascochyta Blight Plant Cell Tissue Organ Culture Embryo Rescue Lens Culinaris Hybrid Embryo 
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© Springer 2007

Authors and Affiliations

  1. 1. South Australian Research and Development Institute (SARDI)AdelaideAustralia
  2. 2.Crop Development CentreUniversity of SaskatchewanSaskatoonCanada

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