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Laboratory Diagnosis of Crimean-Congo Hemorrhagic Fever

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Crimean-Congo Hemorrhagic Fever

Crimean-Congo hemorrhagic fever virus (CCHFV) can induce in humans a severe multisystem syndrome associated with fever, shock, and hemorrhages. In absence of specific clinical symptoms, physicians need a rapid and reliable diagnosis to reinforce the measures of safety (barrier nursing), and possibly to initiate quickly a suitable antiviral treatment. Equally, a differential diagnosis with other agents responsible of hemorrhagic fevers according to epidemiological features has to be undertaken [18, 51].

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References

  1. Ahmed AA, McFalls JM, Hoffmann C, Filone CM, Stewart SM, Paragas J, Khodjaev S, Shermukhamedova D, Schmaljohn CS, Doms RW, Bertolotti-Ciarlet A (2005) Presence of broadly reactive and group-specific neutralizing epitopes on newly described isolates of Crimean-Congo hemorrhagic fever virus. J Gen Virol 86:3327–3336.

    Article  PubMed  CAS  Google Scholar 

  2. Bhagat CI, Lewer M, Prins A, Beilby JP (2000) Effects of heating plasma at 56°C for 30 minutes and 60°C for 60 minutes on routine biochemistry analytes. Ann Clin Biochem 37:802–804.

    Article  PubMed  CAS  Google Scholar 

  3. Blackburn NK, Besselaar TG, Sheperd AJ, Swanepoel R (1987) Preparation and use of monoclonal antibodies for identifying Crimean-Congo hemorrhagic fever virus. Am J Trop Med Hyg 37:392–397.

    PubMed  CAS  Google Scholar 

  4. Borio L, Inglesby T, Peters CJ, Schmaljohn AL, Hughes JM, Jahrling PB, Ksiazek T, Johnson KM, Meyerhoff A, O'Toole T, Ascher MS, Bartlett J, Breman JG, Eitzen EM Jr, Hamburg M, Hauer J, Henderson DA, Johnson RT, Kwik G, Layton M, Lillibridge S, Nabel GJ, Osterholm MT, Perl TM, Russell P, Tonat K; Working Group on Civilian Biodefense (2002) Hemorrhagic fever viruses as biological weapons: medical and public health management. JAMA 287:2391–2405.

    Article  PubMed  Google Scholar 

  5. Burt FJ, Leman PA, Smith JF, Swanepoel R (1998) The use of a reverse transcription-polymerase chain reaction for the detection of viral nucleic acid in the diagnosis of Crimean-Congo haemorrhagic fever. J Virol Methods 70:129–137.

    Article  PubMed  CAS  Google Scholar 

  6. Burt FJ, Leman PA, Abbott JC, Swanepoel R (1994) Serodiagnosis of Crimean-Congo haemorrhagic fever. Epidemiol Infect 113:551–562.

    Article  PubMed  CAS  Google Scholar 

  7. Burt FJ, Spencer DC, Leman PA, Patterson B, Swanepoel R (1996) Investigation of tick-borne viruses as pathogens of humans in South Africa and evidence of Dugbe virus infection in a patient with prolonged thrombocytopenia. Epidemiol Infect 116:353–361.

    Article  PubMed  CAS  Google Scholar 

  8. Burt FJ, Swanepoel R, Braack LE (1993) Enzyme-linked immunosorbent assays for the detection of antibody to Crimean-Congo haemorrhagic fever virus in the sera of livestock and wild vertebrates. Epidemiol Infect 111:547–557.

    Article  PubMed  CAS  Google Scholar 

  9. Burt FJ, Swanepoel R, Shieh WJ, Smith JF, Leman PA, Greer PW, Coffield LM, Rollin PE, Ksiazek TG, Peters CJ, Zaki SR (1997) Immunohistochemical and in situ localization of Crimean-Congo hemorrhagic fever (CCHF) virus in human tissues and implications for CCHF pathogenesis. Arch Pathol Lab Med 121:839–846.

    PubMed  CAS  Google Scholar 

  10. Butenko AM, Chumakov MP (1990) Isolation of Crimean-Congo hemorrhagic fever virus from patients and from autopsy specimens. Arch Virol (Suppl 1):295–301.

    Google Scholar 

  11. Casals J, Tignor GH (1980) The Nairovirus genus. Serological relationships. Intervirol 14:144–147.

    CAS  Google Scholar 

  12. Casey HL (1965) Standard diagnostic complement fixation method and adaptation to microtest. Public Health Monogr 74:1–34.

    PubMed  CAS  Google Scholar 

  13. Clarke D, Casals J (1958) Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7:561–573.

    PubMed  CAS  Google Scholar 

  14. Dickover RE, Herman SA, Saddiq K, Wafer D, Dillon M, Bryson YJ (1998) Optimization of specimen-handling procedures for accurate quantification of levels of human immunodeficiency virus RNA in plasma by reverse transcriptase PCR. J Clin Microbiol 36:1070–1073.

    PubMed  CAS  Google Scholar 

  15. Donets MA, Rezapkin GV, Ivanov AP, Tkachenko EA (1982) Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF). Am J Trop Med Hyg 31:156–162.

    PubMed  CAS  Google Scholar 

  16. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, Günther S (2002) Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 40:2323–2330.

    Article  PubMed  CAS  Google Scholar 

  17. Drosten C, Kummerer BM, Schmitz H, Gunther S (2003) Molecular diagnostics of viral hemorrhagic fevers. Antiviral Res 57:61–87.

    Article  PubMed  CAS  Google Scholar 

  18. Drosten C, Minnak D, Emmerich P, Schmitz H, Reinicke T (2002) Crimean-Congo hemorrhagic fever in Kosovo. J Clin Microbiol 40:1122–1123.

    Article  PubMed  Google Scholar 

  19. Duh D, Saksida A, Petrovec M, Dedushaj I, Avsic-Zupanc T (2006). Novel one-step real-time RT-PCR assay for rapid and specific diagnosis of Crimean-Congo hemorrhagic fever encountered in the Balkans. J Virol Methods 133:175–179.

    Article  PubMed  CAS  Google Scholar 

  20. Gaidamovich SIa, Mel'nikova EE, Shutkova TM, Novokhatskii AS, Turchinskaia AL (1989) Characteristics of the monoclonal antibodies induced by the Crimean hemorrhagic fever virus. Vopr Virusol 34:201–204.

    PubMed  Google Scholar 

  21. Garcia S, Chinikar S, Coudrier D, Billecocq A, Hooshmand B, Crance JM, Garin D, Bouloy M (2006) Evaluation of a Crimean-Congo hemorrhagic fever virus recombinant antigen expressed by Semliki forest suicide virus for IgM and IgG antibody detection in human and animal sera collected in Iran. J Clin Microbiol 35:154–159.

    CAS  Google Scholar 

  22. Gonzalez JP, Camicas JL, Cornet JP, Wilson ML (1998) Biological and clinical responses of West African sheep to Crimean-Congo haemorrhagic fever virus experimental transmission. Res Virol 149:445–455.

    Article  PubMed  CAS  Google Scholar 

  23. Hewson R, Chamberlain J, Mioulet V, Lloyd G, Jamil B, Hasan R, Gmyl A, Gmyl L, Smirnova SE, Lukashev A, Karganova G, Clegg C (2004) Crimean-Congo haemorrhagic fever virus: sequence analysis of the small RNA segments from a collection of viruses world wide. Virus Res 102:185–189.

    Article  PubMed  CAS  Google Scholar 

  24. Hoogstraal H (1979) The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe and Africa. J Med Entomol 15:307–417.

    PubMed  CAS  Google Scholar 

  25. International Air Transport Association (2005) http://www.iata.org/NR/ContentConnector/CS2000/SiteInterface/sites/whatwedo/dangerousgoods/file/Section3.6.2Mar05.pdf.

  26. Johnson KM, Elliott LH, Heymann DL (1981) Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. J Clin Microbiol 14:527–29.

    PubMed  CAS  Google Scholar 

  27. Karabatsos N (1985) International catalog of arboviruses including certain other viruses of vertebrates. American Society of Tropical Medicine and Hygiene, San Antonio, TX.

    Google Scholar 

  28. Karti SS, Odabasi Z, Korten V, Yilmaz M, Sonmez M, Caylan R, Akdogan E, Eren N, Koksal I, Ovali E, Erickson BR, Vincent MJ, Nichol ST, Comer JA, Rollin PE, Ksiazek TG (2004) Crimean-Congo hemorrhagic fever in Turkey. Emerg Inf Dis 10:1379–1384.

    Google Scholar 

  29. Kolman J (1970) Some physical and chemical properties of Uukuniemi virus, strain Potepli-63. Acta Virol 14:159–62.

    PubMed  CAS  Google Scholar 

  30. Kurata T, Hondo R, Sato S, Oda A, Aoyama Y, McCormick JB (1983) Detection of viral antigens in formalin-fixed specimens by enzyme treatment. Ann N Y Acad Sci 420:192–207.

    Article  PubMed  CAS  Google Scholar 

  31. Logan TM, Linthicum KJ, Moulton JR, Ksiazek TG (1993) Antigen-capture enzyme-linked immunosorbent assay for detection and quantification of Crimean-Congo hemorrhagic fever virus in the tick, Hyalomma truncatum. J Virol Methods 42:33–44.

    Article  PubMed  CAS  Google Scholar 

  32. Loutfy MR, Assmar M, Hay Burgess DC, Kain KC (1998) Effects of viral hemorrhagic fever inactivation methods on the performance of rapid diagnostic tests for Plasmodium falciparum. J Infect Dis 178:1852–1855.

    Article  PubMed  CAS  Google Scholar 

  33. Marriott AC, Polyzoni T, Antoniadis A, Nuttall PA (1994) Detection of human antibodies to Crimean-Congo haemorrhagic fever virus using expressed viral nucleocapsid protein. J Gen Virol 75:2157–2161.

    Article  PubMed  CAS  Google Scholar 

  34. Meegan JM, Yedloutschnig RJ, Peleg BA, Shy J, Peters CJ, Walker JS, Shope RE (1987) Enzyme-linked immunosorbent assay for detection of antibodies to Rift valley fever virus in ovine and bovine sera. Am J Vet Res 48:1138–1141.

    PubMed  CAS  Google Scholar 

  35. Nichol ST (2001) Bunyaviruses. In: Knipe DM, Howley PM (eds) Fields Virology, Lippincott Raven, Philadelphia, pp 1603–1633.

    Google Scholar 

  36. Palacios G, Briese T, Kapoor V, Jabado O, Liu Z, Venter M, Zhai J, Renwick N, Grolla A, Geisbert TW, Drosten C, Towner J, Ju J, Paweska J, Nichol ST, Swanepoel R, Feldmann H, Jahrling PB, Lipkin WI (2006) MassTag polymerase chain reaction for differential diagnosis of viral hemorrhagic fever. Emerg Infect Dis 12:692–695.

    PubMed  CAS  Google Scholar 

  37. Peters CJ, LeDuc JW (1991) Bunyaviridae: bunyaviruses, phleboviruses and related viruses. In: Belshe RB (ed.) Textbook of human virology, Mosby Year Book Inc, St Louis, pp 571–614.

    Google Scholar 

  38. Rodriguez LL, Maupin GO, Ksiazek TG, Rollin PE, Khan AS, Schwarz TF, Lofts RS, Smith JF, Noor AM, Peters CJ, Nichol ST (1997) Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates. Am J Trop Med Hyg 57:512–518.

    PubMed  CAS  Google Scholar 

  39. Saijo M, Quing T, Niikura M, Maeda A, Ikegami T, Sakai K, Prehaud C, Kurane I, Morikawa S (2002) Immunofluorescence technique using HeLa cells expressing recombinant nucleoprotein for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus. J Clin Microbiol 40:372–375.

    Article  PubMed  CAS  Google Scholar 

  40. Saijo M, Tang Q, Shimayi B, Han L, Zhang Y, Asiguma M, Tianshu D, Maeda A, Kurane I, Morikawa S (2005) Recombinant nucleoprotein-based serological diagnosis of Crimean-Congo hemorrhagic fever virus infections. J Med Virol 75:295–299.

    Article  PubMed  Google Scholar 

  41. Saijo M, Tang Q, Shimayi B, Han L, Zhang Y, Asiguma M, Tianshu D, Maeda A, Kurane I, Morikawa S (2005) Antigen-capture enzyme-linked immunosorbent assay for the diagnosis of Crimean-Congo hemorrhagic fever using a novel monoclonal antibody. J Med Virol 77:83–88.

    Article  PubMed  CAS  Google Scholar 

  42. Saluzzo JF, Le Guenno B (1987) Rapid diagnosis of human Crimean-Congo hemorrhagic fever and detection of the virus in naturally infected ticks. J Clin Microbiol 25:922–924.

    PubMed  CAS  Google Scholar 

  43. Schwarz TF, Nsanze H, Longson M, Nitschko H, Gilch S, Shurie H, Ameen A, Zahir AR, Acharya UG, Jager G (1996) Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo haemorrahgic fever virus in the United Arab Emirates. Am J Trop Med Hyg 55:190–196.

    PubMed  CAS  Google Scholar 

  44. Shepherd AJ, Leman PA, Swanepoel R (1989) Viremia and antibody response of small African and laboratory animals to Crimean-Congo hemorrhagic fever virus infection. Am J Trop Med Hyg 40:541–547.

    PubMed  CAS  Google Scholar 

  45. Shepherd AJ, Swanepoel R, Gill DE (1988) Evaluation of enzyme-linked immunosorbent assay and reversed passive hemagglutination for detection of Crimean-Congo hemorrhagic fever virus antigen. J Clin Microbiol 26:347–353.

    PubMed  CAS  Google Scholar 

  46. Sheperd AJ, Swanepoel R, Leman PA (1989) Antibody response in Crimean-Congo hemorrhagic fever. Rev Infect Dis 11 (Suppl 4):S801–S806.

    Google Scholar 

  47. Sheperd AJ, Swanepoel R, Leman PA, Sheperd SP (1986) Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus. J Clin Microbiol 24: 654–656.

    Google Scholar 

  48. Swanepoel R (1994) Crimean-Congo haemorrhagic fever. In: Coetzer JAW, Thomson GR, Tustin RC (eds) Infectious diseases of livestock, vol. I. Oxford University Press, Capetown, pp 723–729.

    Google Scholar 

  49. Swanepoel R, Gill DE, Sheperd AJ, Leman PA (1989) The clinical pathology of Crimean-Congo hemorrhagic fever. Rev Infect Dis 11 (Suppl 4):S794–S800.

    PubMed  Google Scholar 

  50. Swanepoel R, Leman PA, Burt FJ, Jardine J, Verwoerd DJ, Capua I, Bruckner GK, Burger WP (1998) Experimental infection of ostriches with Crimean-Congo haemorrhagic fever virus. Epidemiol Infect 121:427–432.

    Article  PubMed  CAS  Google Scholar 

  51. Swanepoel R, Struthers JK, McGillivray GM (1983) Reversed passive hemagglutination and inhibition with Rift Valley fever and Crimean-Congo hemorrhagic fever viruses. Am J Trop Med Hyg 32:610–617.

    PubMed  CAS  Google Scholar 

  52. Tarantola A, Nabeth P, Tattevin P, Michelet C, Zeller H, Incident Management Group (2006) Lookback exercise with imported Crimean-Congo hemorrhagic fever, Senegal and France. Emerg Infect Dis 12:1424–1426.

    Google Scholar 

  53. Tignor GH, Smith AL, Casals J, Ezeokoli CD, Okoli J (1980) Close relationship of Crimean hemorrhagic fever-Congo (CHF-C) virus strains by neutralizing antibody assays. Am J Trop Med Hyg 29:675–685.

    Google Scholar 

  54. Van der Waals FW, Pomeroy KL, Goudsmit J, Asher DM, Gajdusek DC (1986) Hemorrhagic fever virus infections in an isolated rainforest area of Central Liberia. Limitations of the indirect immunofluorescence slide test for antibody screening in Africa. Trop Geogr Med 38:209–214.

    Google Scholar 

  55. Varma MGR, Pudney M, Leake CJ (1975) The establishment of three cell lines from the tick Rhipicephalus appendiculatus (Acari: Ixodidae) and their infection with some arboviruses. J Med Entomol 11:698–706.

    PubMed  CAS  Google Scholar 

  56. Watts DM, Ksiazek TG, Linthicum KJ, Hoogstraal H (1988) Crimean-Congo Hemorrhagic Fever. In: Monath TP (ed.) The arboviruses: epidemiology and ecology, vol. II. CRC Press, Boca Raton, FL, pp 177–222.

    Google Scholar 

  57. Wilson ML, LeGuenno B, Guillaud M, Desoutter D, Gonzalez JP, Camicas JL. (1990) Distribution of Crimean-Congo hemorrhagic fever viral antibody in Senegal: environmental and vectorial correlates. Am J Trop Med Hyg 43:557–566.

    PubMed  CAS  Google Scholar 

  58. Yapar M, Aydogan H, Pahsa A, Besirbellioglu BA, Bodur H, Basustaoglu AC, Guney C, Avci IY, Sener K, Setteh MH, Kubar A (2005) Rapid and quantitative detection of Crimean-Congo hemorrhagic fever virus by one-step real-time reverse transcriptase-PCR. Jpn J Infect Dis 58:358–362.

    PubMed  CAS  Google Scholar 

  59. Zeller HG, Cornet JP, Camicas JL (1997) Crimean Congo hemorrhagic fever in ticks (Acari: Ixodidae) and ruminants: field observations of an epizootic in Bandia, Senegal (1989–1992). J Med Entomol 34:511–516.

    PubMed  CAS  Google Scholar 

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Zeller, H. (2007). Laboratory Diagnosis of Crimean-Congo Hemorrhagic Fever. In: Ergonul, O., Whitehouse, C.A. (eds) Crimean-Congo Hemorrhagic Fever. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6106-6_18

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