A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding
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Putalun, W., Tanaka, H., Shoyama, Y. (2007). Single-chain Fv Antibody Stimulates Biosynthesis of Secondary Metabolites in Plants. In: Verpoorte, R., Alfermann, A., Johnson, T. (eds) Applications of Plant Metabolic Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6031-1_12
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