Abstract
Allozymes and microsatellites were used to assess the level and distribution of genetic variation in grass goby population samples collected from the Venice Lagoon between October 2001 and May 2002. Eighteen enzymatic loci were examined in 434 individuals, 14 of which resulted to be monomorphic, and 4 (GPI-B*, LDH-B*, PGM-A*, PGM-B*) showed 2 alleles scored in 6 individuals only. Comparison with previous data suggests that genetic variation has been eliminated in the Venice Lagoon population during the last few years at three loci. In contrast, analysis of 11 microsatellites in a subset of 192 individuals revealed substantial molecular variation. Analysis of molecular variance showed a lack of genetic differentiation inside the lagoon with respect to site and date of collection, sex, and level of pollution. Significant variation in allelic frequencies was found at microsatellite loci when small (one year old) males were compared to large males (two and three years old), suggesting that a complex population dynamics occurs in this species. The very low level of polymorphism of allozymes could be due to the evolutionary history of the species, or, considering the difference between small and large males, could be the result of recent effects of drift. The second hypothesis is supported by the comparison with previous allozyme studies of the species in the same area, that suggests that loss of heterozygosity at three loci occurred in the last 10 years.
Keywords
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Aizawa, T., M. Hatsumi & K. Wakahama, 1994. Systematic study on the Chenogobius species (Family Gobiidae) by analysis of allozyme polymorphisms. Zoological Science 2: 455–465.
Basaglia, F., 1989. Some aspects of isozymes of lactate dehydrogenase and glucosephosphate isomerase in fish. Comparative Biochemistry and Physiology Part B 92: 213–226.
Basaglia, F., 2002. Multilocus isozyme systems in African lungfish, Protopterus annectens: distribution, differential expression and variation in dipnoans. Comparative Biochemistry and Physiology Part B 131: 89–102.
Bisol, P. M., 2002. Ecogenetic biodiversity in Zosterisessor ophiocephalus from the lagoon of Venice. I: Gene-enzyme polymorphisms. In Campostrini, P. P. (ed.), Scientific Research and Safeguarding of Venice. Corila Research Program 2001 results. Istituto Veneto SS LL AA, Venezia, 601–608.
Callegarini, C. & D. Ricci, 1973. Lactate dehydrogenase (LDH) isozyme in some species of fresh water, euryhaline and salt water teleosts from the Po plain and its sea-coasts. Bollettino di Zoologia 40: 25–30.
Carvalho, G. R. & L. Hauser, 1998. Advances in the molecular analysis of fish population structure. Italian Journal of Zoology 65: 21–33.
Critto, A. & A. Marcomini, 2001. Rischio ecologico e inquinamento chimico lagunare. Libreria Editrice Cafoscarina, Milano.
Excoffier, L., P. Smouse & J. Quattro, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.
Farias, I. P., M. N. Paula-Silva & V. M. Almeida-Val, 1997. No co-expression of LDH-C in Amazon cichlids. Comparative Biochemistry and Physiology Part B 117: 315–319.
Franco, A., S. Malavasi, F. Pranovi, F. Franzoi & P. Torricelli, 2002. Preliminary data on gonadal development and fecundity in the Grass goby, Zosterisessor ophiocephalus (Pallas, 1811) from the Venice Lagoon (Northern Italy). Acta Adriatica 43: 43–48.
Gallini, A., L. Zane & P. M. Bisol, 2005. Isolation and characterization of microsatellites in Zosterisessor ophiocephalus (Perciformes, Gobiidae). Molecular Ecology Notes 5: 24–26.
Goudet, R., 2002. Fstat, version 2.9.3.2. http://www2.unil.ch/izea/softwares/fstat.html.
Hedgecock, D., 1994. Does variance in reproductive success limit effective population sizes of marine organisms? In Beaumont, A. R. (ed.), Genetics and Evolution of Aquatic Organisms. Chapman & Hall, London, 122–134.
Hedgecock, D., V. Chow & R. S. Waples, 1992. Effective population numbers of shell-fish broodstocks estimated from temporal variance in allelic frequencies. Aquaculture 108: 215–232.
Hutchinson, W. F., C. van Oosterhout, S. I. Rogers & G. R. Carvalho, 2003. Temporal analysis of archived samples indicates marked genetic changes in declining North Sea cod (Gadus morhua). Proceeding Royal Society London Ser B: Bio Sci 270: 2125–2132.
Ikeda, M., M. Nunokawa & N. Taniguchi, 2003. Lack of mitochondrial gene flow between populations of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis inhabiting Amami-oshima Island. Fishery Sciences 69: 1162–1168.
Mainardi, D., R. Fiorin, A. Franco, P. Franzoi, O. Giovanardi, A. Granzotto, A. Libertini, S. Malavasi, F. Pranovi, F. Riccato & P. Torricelli, 2002. Fish diversity in the Venice Lagoon: preliminary report. In Campostrini, P. P. (ed.), Scientific Research and Safeguarding of Venice. Corila Research Program 2001 results. Istituto Veneto SS LL AA, Venezia, 583–594.
Maltagliati, F., P. Domenici, C. F. Fosch, P. Cossu, M. Casu & A. Castelli, 2003. Small-scale morphological and genetic differentiation in the Mediterranean killifish Aphanius fasciatus (Cyprinodontidae) from a coastal brackish-water pond and an adjacent pool in northern Sardinia. Oceanological Acta 26: 111–119.
Marconato, A., M. B. Rasotto & C. Mazzoldi, 1996. On the mechanism of sperm release in three gobiid fishes (Teleostei: Gobiidae). Environmental Biology of Fishes 46: 321–327.
Mazzoldi, C., M. Scaggiante, E. Ambrosin & M. B. Rasotto, 2000. Mating system and alternative male mating tactics in the grass goby Zosterisessor ophiocephalus (Teleostei: Gobiidae). Marine Biology 137: 1041–1048.
McKay, S. I. & P. J. Miller, 1991. Isozyme criteria in the testing of phyletic relationships between species of Gobius and related Eastern Atlantic-Mediterranean genera (Teleostei: Gobiidae). Journal of Fish Biology 39: 291–299.
Miller, P. J., 1984. The tokology of Gobioid fishes. In Potts, G. W. & R. J. Wootton (eds), Fish Reproduction: Strategies and Tactics. Academic Press, London, 119–153.
Miller, P. J., M. Serventi, D. Soregaroli, P. Torricelli & G. Gandolfi, 1994. Isozyme genetics and the phylogeny of Italian freshwater gobies (Teleostei: Gobioidei). Journal of Fish Biology 44: 439–451.
Ota, D., M. Marchesan & E. A. Ferrero, 1996. Sperm release behaviour and fertilization in the grass goby. Journal of Fish Biology 49: 246–256.
Penzo, E., G. Gandolfi, L. Bargelloni, L. Colombo & T. Patarnello, 1998. Messinian salinity crisis and the origin of freshwater lifestyle in Western Mediterranean gobies. Molecular Biology and Evolution 15: 1472–1480.
Pezold, F. & J. M. Grady, 1989. A morphological and allozymic analysis of species in the Gobionellus oceanicus complex (Pisces:Gobiidae). Bulletin of Marine Science 45: 648–663.
Planes, S, M. Parroni & C. Chauvet, 1998. Evidence of limited gene flow in three species of coral reef fishes in the lagoon of New Caledonia. Marine Biology 130: 361–368.
Raymond, M. & F. Rousset, 1995. Genepop version 1.2: population genetics software for exact tests and ecumenism. The Journal of Heredity 86: 248–249.
Rianna, E., 2003. Biodiversità in Zosterisessor ophiocephalus di due lagune dell’Alto Adriatico. Bachelor thesis (in Italian), University of Padova.
Roff, D. A. & P. Bentzen, 1989. The statistical analysis of mitochondrial DNA polymorphisms: η 2 and the problem of small samples. Molecular Biology and Evolution 6: 539–545.
Ruzzante, D. E., C. T. Taggart & D. Cook, 1996. Spatial and temporal variation in the genetic composition of a larval cod (Gadus morhua) aggregation: cohort contribution and genetic stability. Canadian Journal Fish Aquatic Science 53: 2695–2705.
Scaggiante, M., C. Mazzoldi, C. W. Petersen & M. B. Rasotto, 1999. Sperm competition and mode of fertilization in the grass goby Zosterisessor ophiocephalus (Teleostei: Gobiidae). The Journal of Experimental Zoology 283: 81–90.
Schneider, S., D. Roessli & L. Excoffier, 2000. Arlequin ver. 2.000. A Software for Population Genetic Data Analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland.
Shaklee, J. B., F. W. Allendorf, D. C. Morizot & G. S. Whitt, 1990. Gene nomenclature for proteincoding loci in fish. Transactions of the American Fisheries Society 119: 2–15.
Shaklee, J. B. & P. Bentzen, 1998. Genetic identification of stocks of marine fish and shellfish. Bulletin of Marine Science 62: 589–621.
Smith, P. J. & Y. Fujio, 1982. Genetic variation in marine teleosts: high variability in habitat specialists and low variability in habitat generalists. Marine Biology 69: 7–20.
Sorice, M. & V. Caputo, 1999. Genetic variation in seven goby species (Perciformes: Gobiidae) assessed by electrophoresis and taxonomic inference. Marine Biology 134: 327–333.
Taylor, M. S. & M. E. Hellberg, 2003. Genetic evidence for local retention of pelagic larvae in a Caribbean reef fish. Science 299: 107–109.
Torricelli, P., S. Malavasi, N. Novarini, F. Pranovi & D. Mainardi, 2000. Elongation of fin rays in parental males of Zosterisessor ophiocephalus (Pisces, Gobiidae). Environmental Biology of Fishes 58: 105–108.
Wallis, G. P. & J. A. Beardmore, 1984a. An electrophoretic study of the systematic relationships of some closely related goby species (Pisces, Gobiidae). Biological Journal of Linnean Society 22: 107–123.
Wallis, G. P. & J. A. Beardmore, 1984b. Genetic variation and environmental heterogeneity in some closely related goby species. Genetica 62: 223–237.
Ward, R. D., D. O. F. Skibinsky & M. Woodwark, 1992. Protein heterozygosity, protein structure, and taxonomic differentiation. Evolutionary Biology 26: 73–157.
Ward, R. D., M. Woodward & D. O. F. Skibinsky, 1994. A comparison of genetic diversity levels in marine, freshwater, and anadromous fishes. Journal of Fish Biology 44: 213–232.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer Science+Business Media B.V.
About this paper
Cite this paper
Bisol, P.M. et al. (2007). Low variation at allozyme loci and differences between age classes at microsatellites in grass goby (Zosterisessor ophiocephalus) populations. In: Viaroli, P., Lasserre, P., Campostrini, P. (eds) Lagoons and Coastal Wetlands in the Global Change Context: Impacts and Management Issues. Developments in Hydrobiology, vol 192. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6008-3_13
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6008-3_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6007-6
Online ISBN: 978-1-4020-6008-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)