This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Buxbaum, E. (2003) Cationic electrophoresis and electrotransfer of membrane glycoproteins. Anal. Biochem. 314, 70–76.
Coughenour, H.D., Spaulding, R.S. and Thompson, C.M. (2004) The synaptic vesicle proteome: a comparative study in membrane protein identification. Proteomics 4, 3141–3155.
Daub, H., Blencke, S., Habenberger, P., Kurtenbach, A., Dennenmoser, J., Wissing, J., Ullrich, A. and Cotten, M. (2002) Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. J. Virol. 76, 8124–8137.
Diao, A., Rahman, D., Pappin, D.J., Lucocq, J. and Lowe, M. (2003) The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation. J. Cell Biol. 160, 201–212.
Fujiki, Y., Hubbard, A.L., Fowler, S. and Lazarow, P.B. (1982) Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J. Cell Biol. 93, 97–102.
Godl, K., Wissing, J., Kurtenbach, A., Habenberger, P., Blencke, S., Gutbrod, H., Salassidis, K., Stein-Gerlach, M., Missio, A., Cotten, M. and Daub, H. (2003) An efficient proteomics method to identify the cellular targets of protein kinase inhibitors. Proc. Natl. Acad. Sci. U.S.A. 100, 15434–15439.
Hartinger, J., Stenius, K., Hogemann, D. and Jahn, R. (1996) 16-BAC/SDS-PAGE: a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal. Biochem. 240, 126–133.
Kramer, M.L. (2006) A new multiphasic buffer system for benzyldimethyl-n-hexadecylammonium chloride polyacrylamide gel electrophoresis of proteins providing efficient stacking. Electrophoresis 27, 347–356.
Luche, S., Santoni, V. and Rabilloud, T. (2003) Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249–253.
Macfarlane, D.E. (1983) Use of benzyldimethyl-n-hexadecylammonium chloride (“16-BAC”), a cationic detergent, in an acidic polyacrylamide gel electrophoresis system to detect base labile protein methylation in intact cells. Anal. Biochem. 132, 231–235.
Macfarlane, D.E. (1989) Two dimensional benzyldimethyl-n-hexadecylammonium chloride – sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures. Anal. Biochem. 176, 457–463.
Moebius, J., Zahedi, R.P., Lewandrowski, U., Berger, C., Walter, U. and Sickmann, A. (2005) The human platelet membrane proteome reveals several new potential membrane proteins. Mol. Cell Proteomics 4, 1754–1761.
Molloy, M.P. (2000) Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients. Anal. Biochem. 280, 1–10.
Olsson, I., Larsson, K., Palmgren, R. and Bjellqvist, B. (2002) Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension. Proteomics 2, 1630–1632.
Otto, H., Dreger, M., Bengtsson, L. and Hucho, F. (2001) Identification of tyrosine-phosphorylated proteins associated with the nuclear envelope. Eur. J. Biochem. 268, 420–428.
Rais, I., Karas, M. and Schagger, H. (2004) Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification. Proteomics 4, 2567–2571.
Reinders, J., Zahedi, R.P., Pfanner, N., Meisinger, C. and Sickmann, A. (2006a) Toward the Complete Yeast Mitochondrial Proteome: Multidimensional Separation Techniques for Mitochondrial Proteomics. J. Proteome Res. 5, 1543–1554.
Reinders, Y., Schulz, I., Graf, R. and Sickmann, A. (2006b) Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches. J. Proteome Res. 5, 589–598.
Santoni, V., Molloy, M. and Rabilloud, T. (2000) Membrane proteins and proteomics: un amour impossible? Electrophoresis 21, 1054–1070.
Unlu, M., Morgan, M.E. and Minden, J.S. (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18, 2071–2077.
Zahedi, R.P., Meisinger, C. and Sickmann, A. (2005) Two-dimensional benzyldimethyl-n-hexadecylammonium chloride/SDS-PAGE for membrane proteomics. Proteomics 5, 3581–3588.
Zahedi, R.P., Sickmann, A., Boehm, A.M., Winkler, C., Zufall, N., Schonfisch, B., Guiard, B., Pfanner, N. and Meisinger, C. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol. Biol. Cell 17, 1436–1450.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this chapter
Cite this chapter
Zahedi, R., Moebius, J., Sickmann, A. (2007). Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics. In: Bertrand, E., Faupel, M. (eds) Subcellular Proteomics. Subcellular Biochemistry, vol 43. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5943-8_2
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5943-8_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5942-1
Online ISBN: 978-1-4020-5943-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)