Abstract
Recent inelastic and quasi-elastic neutron scattering measurements of water in cell preparations has provided information on the interfacial (or bound) water molecules. The experiments show that the interfacial water molecules can be readily distinguished from those in the bulk (bulk water), especially using inelastic neutron scattering data over the 20-130 meV range. Studies of intact biological systems - whole cells and tissues - demonstrated the feasibility of using these methods to assess the degree of interfacial water and their potential for monitoring physiological changes. Here we also describe the effect of heat shock and osmotic stress on yeast and E. coli cells, and show that the interfacial water content increases with elevated osmolarity and heat shock, and decreases under hypoosmotic conditions.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
4. References
Ellis, J. R (2001) Curr. Opin. Struct. Biol. 1 1, 114-119.
Burg, M. C. (2000) Cell Physiol. Biochem. 10, 251-256.
Verkman, A. S. (2002) TIBS 27, 27-33.
Hall, D. and Minton, A.P. (2003) Biochim. Biophys. Acta 1649, 127-139.
Ruffle, S. V., Michalarias, I., Li, J. C., and Ford, R. C. (2002) J. Amer. Chem. Soc. 124, 565-569.
Beta, I. A., Kolesnikov, A. I., Michalarias, I., Wu, G. L., Ford, R. C., and Li, J. C. (2003) Can. J. Phys. 81, 367-371.
Michalarias, I., Beta, I. A., Ford, R. C., Ruffle, S. V., and Li, J. C. (2002) Appl. Phys. A 74, 1242-1244.
Michalarias, I., Beta, I. A., Li, J. C., Ruffle, S. V., and Ford, R. C. (2002) J. Mol. Liq. 101, 19-26.
Li, J. C. (1996) J. Chem. Phys. 105, 6733-6755.
Li, J. C. and Tomkinson, J. (1999) In molecular dynamics from classical to quantum methods, Theoretical and Computational Chemistry. (Eds. P. B. Balbuena and J. M. Seminario), Vol. 7. pp. 471-512.
Beta, I. A., Michalarias, I., Li, J. C., Ford, R. C., and Bellissent-Funel, M.-C. (2003) Chem. Phys. 292, 451-454.
Dellerue, S. and Bellissent-Funel, M. C. (2000) Chem. Phys. 258, 315-325.
Russo, D., Baglioni, P., Peroni, E., and Texeira, J. (2003) Chem. Phys. 292, 235-245.
Pieper, J., Charalambopoulou, G., Steriotis, T., Vasenkov, S., Desmedt, A. and Lechner, R.E. (2003) Chem. Phys. 292, 465-476.
Esposito, L., Vitagliano, L., Sica, F., Sorrentino, G., Zagari, A., and Mazzarella, L. (2000) J. Mol. Biol. 297, 713-792.
Clark, G. R., Squire, C. J., Baker, L. J., Martin, R. F., and White, J. (2000) Nucl. Acids Res. 28, 1259-1265.
Soler-Lopez, M., Malinia, L., and Subirana, J. A. (2000) J. Biol. Chem. 275, 23034-23044.
Goupil-Lamy, A. V., Smith, J. C., Yunoki, J., Parker, S. F., and Kataoka, M. (1997) J. Am. Chem. Soc. 119, 9268-9273.
Ford, R. C., Ruffle, S. V., Ramirez-Cuesta, A., Michalarias, I., Beta, I. A., Miller, A., and Li, J. C. (2004) J. Amer. Chem. Soc. 126, 4682-4688.
Ford, R. C., Ruffle, S. V., Michalarias, I., Beta, I. A., Ramirez-Cuesta, A., and Li, J. C. (2004) J. Mol. Recogn. 17, 505-511.
Mathews, B. W., Sigler, P. B., Henderson, R., Blow, D. M. (1967) Nature 214, 652-656.
Lovelock, J. E. (1953) Biochim. Biophys. Acta 11, 28-36.
Minton, A. P. (2000) Biophys. J. 78, 101-109.
Eggers, D. K. and Valentine, J. S. (2001) Protein Sci. 10, 250-261.
Fitter, J. (2003) Chem. Phys. 292, 405-411.
Tarek, M., Neumann, D. A. and Tobias, D. J. (2003) Chem. Phys. 292, 435-443.
Record, M. T., Courtenay, E. S., Cayley, D. S., and Guttman, H. J. (1998) TIBS 23, 143-147.
Cayley, D. S., Guttman, H. J., and Record, M. T. (2000) Biophys. J. 78, 1748-1764.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Ford, R.C., Li, J. (2007). Recent progresses in understanding of water interacting with biomolecules, and inside living cells and tissues. In: Rzoska, S.J., Mazur, V.A. (eds) Soft Matter under Exogenic Impacts. NATO Science Series II: Mathematics, Physics and Chemistry, vol 242. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5872-1_28
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5872-1_28
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5871-4
Online ISBN: 978-1-4020-5872-1
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)