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Effect of phosphorous on nodulation and nitrogen fixation by Phaseolus vulgaris

  • Conference paper
First International Meeting on Microbial Phosphate Solubilization

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 102))

Abstract

The impact of phosphorous on plant growth and symbiotic N2 fixation in common bean (P. vulgaris) plants was investigated. Plants inoculated with R. tropici CIAT899 were grown with six P dosage. The P increased plant growth, nodule mass, nitrogenase activity (ARA) and P content, and decreased amino acids and total soluble sugars in the vegetative organs (root, shoot and nodule). The root growth proved less sensitive to P deficiency than did shoot growth, and the leaf area was inhibited at low P. The optimal amount for this symbiosis was 1.5 mM P, this treatment augmented nodule ARA some 20-fold and plant ARA some 70-fold with respect to control.

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References

  • Almeida J P, Hartwig U A, Freshner M, Nösberger J and Lüsher A 2000 Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L.). J. Exp. Bot. 51, 1289–1297.

    Article  PubMed  CAS  Google Scholar 

  • Bieleski R L 1973 Phosphate pools, phosphate transport, and phosphate availability. Annu. Rev. Plant Physiol. 24, 225–252.

    Article  CAS  Google Scholar 

  • Burauel P, Wieneke J and Führ F 1990 Carbohydrate status in roots of two soybean cultivars: a possible parameter to explain different efficiencies concerning phosphate uptake. In Genetics Aspects of Plant Mineral Nutrition. Ed. El Bassan. pp. 111–116. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • CIAT, 1992. Constraints to and opportunities for improving bean production. A planning document 1993–98. An achievement document 1987–92. CIAT, Cali Colombia.

    Google Scholar 

  • Herdina J A and Silsbury J H 1990 Estimating nitrogenase activity of faba bean (Vicia faba L.) by acetylene reduction assay. Aust. J. Plant Physiol. 17, 489–502.

    Article  CAS  Google Scholar 

  • Irigoyen J J, Emerich D W and Sánchez-Díaz M 1992 Water stress induced changes in concentrations of proline and total soluble sugar in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 84, 55–60.

    Article  CAS  Google Scholar 

  • Israel D W 1987 Investigation of the role of phosphorus in symbiotic dinitrogen fixation. Plant Physiol. 84, 835–840.

    Article  PubMed  CAS  Google Scholar 

  • Khadri M, Pliego L, Soussi M, Lluch C and Ocaña A 2001 Ammonium assimilation and ureide metabolism in common bean nodules under salt stress. Agronomie 21, 635–643.

    Article  Google Scholar 

  • Kolawole G O and Kang B T 1997 Effect of seed size and phosphorus fertilization on growth of select legumes. Commun. Soil Sci. Plant Anal. 28, 1223–1235.

    CAS  Google Scholar 

  • Lachica M, Aguilar A and Yañez J 1973 Análisis foliar. Métodos utilizados en la Estación Experimental del Zaidín C.S.I.C. (II). Anal. Edaf. Agrobiol. XXXII, 1033–1047.

    Google Scholar 

  • Larson C M 1994 Responses of the nitrate uptake system to external nitrate availability: a whole plant perspective. In A Whole Plant Perspective on Carbon-Nitrogen Interactions. Eds. J Roy and E Garner. pp. 31–45. SBB Academic Publish, The Hague Netherlands.

    Google Scholar 

  • Lynch J, Läuchli A and Epstein E 1991 Vegetative growth of the common bean in response to phosphorus nutrition. Crop Sci. 31, 380–387.

    Article  CAS  Google Scholar 

  • Pereira P A A and Bliss F A 1987 Nitrogen fixation and plant growth of common bean (Phaseolus vulgaris L.) at different levels of phosphorus availability. Plant Soil 104, 79–84.

    Article  CAS  Google Scholar 

  • Ribet J and Drevon J J 1996 The phosphorus requirement of N2 fixation and urea-fed Acacia mangium. New Phytol. 132, 383–396.

    Article  CAS  Google Scholar 

  • Rigaud J and Puppo A 1975 Indole-3-acetic acid catabolism by soybean bacteroids. J. Gen. Microbiol. 88, 223–228.

    Google Scholar 

  • Robson A D 1983 Mineral nutrition. In Nitrogen Fixation. Ed. W J Broughton. pp. 36–55. Oxford University Press, Great Britain.

    Google Scholar 

  • Rychter A M and Randall D D 1994 The effect of phosphate deficiency on carbohydrate metabolism in bean roots. Physiol. Plant 91, 383–388.

    Article  CAS  Google Scholar 

  • Tabatabai M A and Bremner J M 1969 Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1, 301–307.

    Article  CAS  Google Scholar 

  • Vadez V, Lasso J H, Beck D P and Drevon J J 1999 Variability of N2-fixation in common bean under P deficiency is related to P use efficiency. Euphytica 106, 231–242.

    Article  Google Scholar 

  • Wanke M, Ciereszko I, Podbiekowska M and Rychter A M 1998 Response to phosphate deficiency in bean (Phaseolus vulgaris L.). Respiratory metabolism, sugar localization and changes in ultrastructure of bean root cells. Ann. Bot. 82, 809–818.

    Article  CAS  Google Scholar 

  • Yemm E W and Cocking E C 1955 The determination of amino acids with ninhydrin. Analystic 80, 209–213.

    Article  CAS  Google Scholar 

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E. Velázquez C. Rodríguez-Barrueco

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Olivera, M., Tejera, N., Iribarne, C., Ocaña, A., Lluch, C. (2007). Effect of phosphorous on nodulation and nitrogen fixation by Phaseolus vulgaris . In: Velázquez, E., Rodríguez-Barrueco, C. (eds) First International Meeting on Microbial Phosphate Solubilization. Developments in Plant and Soil Sciences, vol 102. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5765-6_23

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