Skip to main content

Phaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil

  • Conference paper

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 102))

Abstract

The genus Phaseolus includes several species indigenous to American continent that belong to family Leguminosae. This genus includes several species, some of them only cultivated in American countries. This is the case of Phaseolus lunatus. This plant can be nodulated by fast and slow growing rhizobia. At the moment the fast growing species nodulating Phaseolus commonly belong to genus Rhizobium and more rarely to Sinorhizobium fredii. A strain, LMTR32, isolated from Phaseolus lunatus growing in Peru soils showed a high ability to solubilize bicalcium phosphate from YED-P plates. The 16S rRNA sequence of this strain showed a 100% similarity with the type strain of Sinorhizobium meliloti. The LMW RNA profile of this strain is identical to that of type strain of Sinorhizobium meliloti and confirms that the strain LMTR32 belongs to this species. More studies are necessary in order to establish the prevalence of this species in nodules of Phaseolus lunatus in Perú, and, in the future, it will be very interesting to perform wider taxonomic studies of rhizobia nodulating Phaseolus in different American countries.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Amarger N, Macheret V and Laguerre G 1997 Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov. from Phaseolus vulgaris nodules. Int. J. Syst. Bacteriol. 47, 996–1006.

    PubMed  CAS  Google Scholar 

  • Bergersen F J 1961 The growth of Rhizobium in synthetic media. Aust. J. Biol. Sci. 14, 349–360.

    CAS  Google Scholar 

  • Beringer J E 1974 R factors transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84, 188–198.

    PubMed  CAS  Google Scholar 

  • Bidle K D and Fletcher M 1995 Comparison of free-living and particle-associated bacterial communities in the Chesapake Bay by stable low-molecular-weight RNA analysis. Appl. Environ. Microbiol. 61, 944–952.

    PubMed  CAS  Google Scholar 

  • Cruz-Sánchez J M, Velázquez E, Mateos P and Martínez-Molina E 1997 Enhancement of resolution of low molecular weight RNA profiles by staircase electrophoresis. Electrophoresis 18, 1909–1911.

    Article  PubMed  Google Scholar 

  • de Freitas J R, Banerjee M R and Germida J J 1997 Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorous uptake in canola (Brassica napus L.). Biol. Fertil. Soils 24, 358–364.

    Article  Google Scholar 

  • Haas H, Budowle B and Weiler G 1994 Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments. Electrophoresis 15, 153–158.

    Article  PubMed  CAS  Google Scholar 

  • Halder A K, Mishra A K and Chakrabartty P K 1990 Solubilization of phosphatic compounds by Rhizobium. Indian J. Microbiol. 30, 311–314.

    Google Scholar 

  • Halder A K, Mishra A K, Bhattacharyya P and Chakrabartty P K 1993 Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. J. Gen. Appl. Microbiol. 36, 81–92.

    Google Scholar 

  • Herrera-Cervera J A, Caballero-Mellado J, Laguerre G, Tichy H V, Requena N, Amarger N, Martínez-Romero E, Olivares J and Sanjuán J 1999 At least five rhizobial species nodulate Phaseolus vulgaris in a Spanish soil. FEMS Microbiol. Ecol. 30, 87–97.

    Article  CAS  Google Scholar 

  • Höfle M G 1988 Identification of bacteria by low molecular weight RNA profiles: A new chemotaxonomic approach. J. Microbiol. Meth. 8, 235–248.

    Article  Google Scholar 

  • Jonhson F J 1990 Detection method of nitrogen (total) in fertilizers. In Methods of Analysis of the Association of Official Analytical Chemists. Ed. K Elrich. pp. 17–19. Association of Official Analytical Chemists, USA.

    Google Scholar 

  • Jordan D C 1984 Family III, Rhizobiaceae Conn 1938 321AL. In Bergeýs Manual of Systematic Bacteriology Vol. 1. Eds. N R Krieg and J G Holt. pp. 234–244. Williams & Wilkins, Baltimore.

    Google Scholar 

  • Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P and Pardo M A 1991 Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int. J. Syst. Bacteriol. 41, 417–426.

    PubMed  Google Scholar 

  • Laguerre G, Nour S M, Macheret V, Sanjuán J, Drouin P and Amarguer N 2001 Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147, 981–993.

    PubMed  CAS  Google Scholar 

  • Matos G and Zúñiga D 2002 Comportamiento de cepas nativas de rhizobios aisladas de la costa del Perúen dos cultivares de pallar (Phaseolus lunatus). Ecol. Appl. 1, 19–24.

    Google Scholar 

  • Mhamdi R, Laguerre G, Elarbi-Aouani M, Mars M and Amarger N 2002 Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils. FEMS Microbiol. Ecol. 41, 77–84.

    Article  CAS  PubMed  Google Scholar 

  • Pearson W R and Lipman D J 1988 Improved tools for biological sequence comparison. Proc. Nat. Acad. Sci. USA 85, 2444–2448.

    Article  PubMed  CAS  Google Scholar 

  • Peix A., Rivas-Boyero A.A., Mateos P.F., Rodríguez-Barrueco C., Martínez-Molina E. and Velázquez E 2001 Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol. Biochem. 33, 103–110.

    Article  CAS  Google Scholar 

  • Rivas R, Velázquez E, Valverde A, Mateos P F and Martínez-Molina E 2001 A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22, 1086–1089.

    Article  PubMed  CAS  Google Scholar 

  • Rivas R, Velázquez E, Willems A, Vizcaíno N, Subba-Rao N S, Mateos P F, Gillis M, Dazzo F B and Martínez-Molina E 2002 A new species of Devosia that forms a nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L. f.) Druce. Appl. Environ. Microbiol. 68, 5217–5222.

    Article  PubMed  CAS  Google Scholar 

  • Rodríguez-Navarro D N, Buendía A M, Camacho M, Lucas M and Santamaría C 2000 Characterization of Rhizobium spp. bean isolates from southwest of Spain. Soil. Biol. Biochem. 32, 1601–1613.

    Article  Google Scholar 

  • Sadowsky M J, Cregan P and Keyser H H 1988 Nodulation and nitrogen fixation efficacy of Rhizobium fredii with Phaseolus vulgaris genotypes. Appl. Environ. Microbiol. 54, 1907–1910.

    PubMed  Google Scholar 

  • Segovia L, Young J P W and Martínez-Romero E 1993 Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int. J. Syst. Bacteriol. 43, 374–377.

    PubMed  CAS  Google Scholar 

  • Sprinzl M, Moll J, Meissner F and Hatmann T 1985 Compilation of tRNA sequences. Nucleic Acid Res. 13, 1–49.

    Article  Google Scholar 

  • van Berkum P, Beyene D, Bao G, Campbell T A and Eardly B D 1998 Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]. Int. J. Syst. Bacteriol. 48, 13–22.

    Article  PubMed  Google Scholar 

  • Velázquez E, Martínez-Romero E, Rodríguez-Navarro D N, Trujillo M E, Daza A, Mateos P F, Martinez-Molina E and Van Berkum P 2001a Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low molecular weight RNA. Appl. Environ. Microbiol. 67, 1008–1010.

    Article  PubMed  Google Scholar 

  • Velázquez E, Trujillo M E, Peix A, Palomo J L, García-Benavides P, Mateos P, Ventosa A and Martínez-Molina E 2001b Stable low molecular weight RNA analyzed by staircase electrophoresis, a molecular signature for both prokaryotic and eukaryotic microorganisms. Syst. Appl. Microbiol. 24, 490–499.

    Article  PubMed  Google Scholar 

  • Vincent J M 1970 The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of Root-Nodule. Ed. J M Vincent. pp. 1–13. Blackwell Scientific Publications, Oxford.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

E. Velázquez C. Rodríguez-Barrueco

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this paper

Cite this paper

Ormeño, E. et al. (2007). Phaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil. In: Velázquez, E., Rodríguez-Barrueco, C. (eds) First International Meeting on Microbial Phosphate Solubilization. Developments in Plant and Soil Sciences, vol 102. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5765-6_21

Download citation

Publish with us

Policies and ethics