Abstract
The registration of luminescence emission spectra at different decay times from the excitation pulse is called time-resolved (gated) luminescence spectroscopy. This technique makes it possible to distinguish between short and long decay components. In particular, a long decay emission (phosphorescence) can be separated from the background signal, which most often consists of short decay fluorescence and scattering; because measurements do not occur until a certain time has elapsed from the moment of excitation. Time-resolved spectroscopy finds applications in many areas such as molecular phosphorescence, fingerprint detection, immunoassay, enzyme activity measurements, etc. In this presentation we will focus on fingerprint detection with time-resolved luminescence imaging using Eu(III) and Tb(III). These two chemicals react with Ruhemann’s Purple, RP (RP is the reaction product of ninhydrin with amino acid glycine), and are suitable for this purpose. They have a relatively long luminescence decay of millisecond-order. Instrumentation and fingerprint samples developed by this technique will be presented and discussed.
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Alaoui, I.M. (2007). TIME-RESOLVED LUMINESCENCE IMAGING AND APPLICATIONS. In: Byrnes, J. (eds) Imaging for Detection and Identification. NATO Security through Science Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5620-8_12
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DOI: https://doi.org/10.1007/978-1-4020-5620-8_12
Publisher Name: Springer, Dordrecht
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Online ISBN: 978-1-4020-5620-8
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