Skip to main content

Design of Metabolite Recovery by Variations of the Metabolite Profiling Protocol

  • Conference paper
Concepts in Plant Metabolomics

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ausloos, P., Clifton, C.L., Lias, S.G., Mikaya, A.I., Stein, S.E., Tchekhovskoi, D.V., Sparkman, O.D., Zaikin, V., and Zhu, D., 1999, The critical evaluation of a comprehensive mass spectral library, J. Am. Soc. Mass Spectrom. 10: 287-299.

    Article  CAS  PubMed  Google Scholar 

  • Bieleski, R.L., 1964, Problem of halting enzyme action when extracting plant tissues, Anal. Biochem. 9:431-442.

    Article  CAS  PubMed  Google Scholar 

  • Birkemeyer, C., Kolasa, A., and Kopka, J., 2003, Comprehensive chemical derivatization for gas chromatography-mass spectrometry-based multi-targeted profiling of the major phytohormones, J. Chromatogr. A 993:89-102.

    Article  Google Scholar 

  • Birkemeyer, C., Luedemann, A., Wagner, C., Erban, A., and Kopka, J., 2005, Metabolome analysis: the potential of in vivo labeling with stable isotopes for metabolite profiling, Trends Biotechnol. 23:28-33.

    Article  CAS  PubMed  Google Scholar 

  • Colebatch, G., Desbrosses, G., Ott, T., Krusell, L., Kloska, S., Kopka, J., and Udvardi, M.K., 2004, Global changes in transcription orchestrate metabolic differentiation during symbiotic nitrogen fixation in Lotus japonicus, Plant J. 39:487-512.

    Article  PubMed  Google Scholar 

  • Fiehn, O., Kopka, J., Trethewey, R.N., and Willmitzer, L., 2000a, Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry, Anal. Chem. 72:3573-3580.

    Article  CAS  Google Scholar 

  • Fiehn, O., Kopka, J., Dörmann, P., Altmann, T., Trethewey, R.N., and Willmitzer, L., 2000b, Metabolite profiling for plant functional genomics, Nat. Biotechnol. 18:1157-1161.

    Article  CAS  Google Scholar 

  • Gullberg, J., Jonsson, P., Nordstrom, A., Sjostrom, M., and Moritz, T., 2004, Design of experiments: an efficient strategy to identify factors influencing extraction and derivatization of Arabidopsis thaliana samples in metabolomic studies with gas chromatography/mass spectrometry, Anal. Biochem. 331:283-295.

    Article  CAS  PubMed  Google Scholar 

  • Harada, K., Fukusaki, E., Bamba, T., and Kobayashi, A. 2004, In-vivo 15N-enrichment of metabolites in Arabidopsis cultured cell T87 and its application for metabolomics, Third international congress on plant metabolomics, #23.

    Google Scholar 

  • Kopka, J., Fernie, A.F., Weckwerth, W., Gibon, Y., and Stitt, M., 2004, Metabolite profiling in Plant Biology: Platforms and Destinations, Genome Biol. 5 (6):109-117.

    Article  PubMed  Google Scholar 

  • Kopka, J., Schauer, N., Krueger, S., Birkemeyer, C., Usadel, B., Bergmüller, E., Dörmann, P., Gibon, Y., Stitt, M., Willmitzer, L., Fernie, A.R., and Steinhauser, D., 2005, GMD@CSB.DB: The Golm Metabolome Database, Bioinformatics (doi:10.1093/bioinfor-matics/bti236).

    Google Scholar 

  • Mashego, M.R., Wu, L., Van Dam, J.C., Ras, C., Vinke, J.L., Van Winden, W.A., Van Gulik, W.M., Heijnen, J.J., 2004, MIRACLE: mass isotopomer ratio analysis of U-13C-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites, Biotech. Bioeng. 85:620-628.

    Article  CAS  Google Scholar 

  • Roessner, U., Wagner, C., Kopka, J., Trethewey, R.N., and Willmitzer, L., 2000, Technical advance: simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry, Plant J. 23:131-142.

    Article  CAS  PubMed  Google Scholar 

  • Roessner, U., Luedemann, A., Brust, D., Fiehn, O., Linke, T., Willmitzer, L., and Fernie, A.R., 2001, Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems, Plant Cell 13:11-29.

    Article  CAS  PubMed  Google Scholar 

  • Roessner-Tunali, U., Hegemann, B., Lytovchenko, A., Carrari, F., Bruedigam, C., Granot, D., and Fernie, A.R., 2003, Metabolic profiling of transgenic tomato plants overexpressing hexokinase reveals that the influence of hexose phosphorylation diminishes during fruit development, Plant Physiol. 133:84-99.

    Article  CAS  PubMed  Google Scholar 

  • Schauer, N., Steinhauser, D., Strelkov, S., Schomburg, D., Allison, G., Moritz, T., Lundgren, K., Roessner-Tunali, U., Forbes, M.G., Willmitzer, L., Fernie, A.R., and Kopka, J., 2005, GC-MS libraries for the rapid identification of metabolites in complex biological samples, FEBS Letters (in press).

    Google Scholar 

  • Stein, S.E., 1999, An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data, J. Am. Soc. Mass Spectrom. 10:770-781.

    Article  CAS  Google Scholar 

  • Sumner, L.W., Mendes, P., and Dixon, R.A., 2003, Plant metabolomics: large-scale phytochemistry in the functional genomics era, Phytochemistry 62:817-836.

    Article  CAS  PubMed  Google Scholar 

  • Wagner, C., Sefkow, M., and Kopka, J., 2003, Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles, Phytochemistry 62:887-900.

    Article  CAS  PubMed  Google Scholar 

  • Weckwerth, W., Tolstikov, V., and Fiehn, O., 2001, Proceedings of the 49th ASMS Confe-rence on Mass Spectrometry and Allied Topics, ASMS, Chicago, pp. 1-2.

    Google Scholar 

  • Weckwerth, W., Wenzel, K., and Fiehn, O., 2004, Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co-regulation in biochemical networks, Proteomics 4:78-83.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this paper

Cite this paper

Birkemeyer, C., Kopka, J. (2007). Design of Metabolite Recovery by Variations of the Metabolite Profiling Protocol. In: Nikolau, B.J., Wurtele, E.S. (eds) Concepts in Plant Metabolomics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5608-6_4

Download citation

Publish with us

Policies and ethics