Optimizing Medium for Transient Transfection
During the development of a transient transfection platform, it was observed that several commonly used components were able to support high transfection efficiency and protein production when added at certain steps of the transfection process. Transfection could be inhibited or completely diminished if such critical components were present at other steps of the process. Our data demonstrated that a transfection protocol can be as critical to efficient polyethylenimine (PEI) mediated transient transfection of human embryonic kidney (HEK) 293 EBNA cells as the transfection medium.
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