Abstract
Initial attempts to grow GS NS0 cells in the serum-free media provided by Cambrex failed owing to a lack of certain additions. It was decided to evaluate the addition of cholesterol, cholesterol encapsulated with a cyclodextrin carrier and Glutamine Synthetase Expression Supplements (GSES). Aim was to evaluate if the adaptation time could be reduced, while still maintaining equal to higher viable cell density and IgG antibody production. It was found that GS NS0 cells required the addition of cholesterol to sustain growth in the medium. The addition of cholesterol encapsulated with cyclodextrin with the GSES additives reduced adaptation time to 50 days from 127 days for cholesterol alone.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Keen, M. J.; Steward, T. W. Adaptation of cholesterol requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium. Cytotechnology 1995, 17, 203–211.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Clee, P., Al-Rubeai, M. (2007). Media and Cell Manipulation Approaches to the Reduction of Adaptation Time in GS NS0 Cell Line. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_63
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_63
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)