Media and Cell Manipulation Approaches to the Reduction of Adaptation Time in GS NS0 Cell Line

  • Paul Clee
  • Mohamed Al-Rubeai
Conference paper

Abstract

Initial attempts to grow GS NS0 cells in the serum-free media provided by Cambrex failed owing to a lack of certain additions. It was decided to evaluate the addition of cholesterol, cholesterol encapsulated with a cyclodextrin carrier and Glutamine Synthetase Expression Supplements (GSES). Aim was to evaluate if the adaptation time could be reduced, while still maintaining equal to higher viable cell density and IgG antibody production. It was found that GS NS0 cells required the addition of cholesterol to sustain growth in the medium. The addition of cholesterol encapsulated with cyclodextrin with the GSES additives reduced adaptation time to 50 days from 127 days for cholesterol alone.

Keywords

Cholesterol Glutamine Myeloma Cyclodextrin Verse 

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References

  1. Keen, M. J.; Steward, T. W. Adaptation of cholesterol requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium. Cytotechnology 1995, 17, 203–211.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Paul Clee
    • 1
  • Mohamed Al-Rubeai
    • 1
    • 2
  1. 1.Department of Chemcial EngineeringUniversity of BirminghamBirmingham
  2. 2.Department of Chemcial and Biochemcial EngineeringUniversity College DublinBelfieldIreland

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