Large-scale Expansion of Cells for Use as Therapeutic Agents

  • Juan Melero-Martin
  • Shanmugapriya Santhalingam
  • Mohamed Al-Rubeai
Conference paper

Abstract

Expansion of cell population in vitro has become an essential step for the tissue engineering processing of articular cartilage and there is an urgent need to develop optimal culture conditions for chondrocytes. This presentation focuses on using a mathematical approach coupled with small-scale experiments to determine the optimal process parameters for cell expansion of chondroprogenitor cells and comparing the process to CHO cells expansion.

Keywords

Penicillin Interferon Streptomycin 

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References

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    Dowthwaite, G.P., Bishop, J.C., Redman, S.N., Khan, I.M., Rooney, P., Evans, D.J., Haughton, L., Bayram, Z., Boyer, S., Thomson, B. et al., 2004, The surface of articular cartilage contains a progenitor cell population, J. Cell Sci., 117, 889–897CrossRefGoogle Scholar
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    Martin, J.M.M., Smith, M. and Al-Rubeai, M., 2005, Cryopreservation and in vitro expansion of chondroprogenitor cells isolated from the superficial zone of articular cartilage, Biotechnol. Prog., 21, 168–177CrossRefGoogle Scholar
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    Skehan, P., and Friedman, S.J., 1984, Non-exponential growth by mammalian cells in culture, Cell Tissue Kinet., 17, 335–343Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Juan Melero-Martin
    • 1
  • Shanmugapriya Santhalingam
    • 1
  • Mohamed Al-Rubeai
    • 1
    • 2
  1. 1.Department of Chemical EngineeringThe University of BirminghamEdgbastonUK
  2. 2.Department of Chemical and Biochemical Engineering and Centre for Synthesis and Chemical BiologyUniversity College DublinBelfieldIreland

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