Comparison of Different Transient Retroviral Transfection Systems

  • V. Kleff
  • I. Rattmann
  • C. Ludwig
  • M. Flasshove
  • T. Moritz
  • B. Opalka
Conference paper

Abstract

Oncoretroviral preparations were produced by PEI and CaPO4 precipitationmediated transient transfection of producer cells. For optimizing the latter transfection method, the doses of different supporting agents were varied and incubation times were altered. Best results were achieved with a modified CaPO4 protocol. For the production of foamy and lentiviral preparations different transient transfection agents were tested. Foamyviral supernatants had best titers after transfection with Polyfect and CaPO4, while lentiviral preparations generated with CaPO4 achieved the best results.

Keywords

Leukemia Butyrate Chloroquine Stomatitis 

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References

  1. Baum C, Hegewisch-Becker S, Eckert HG, Stocking C, Ostertag W. Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells. J Virol, 1995, 69: 7541–7547.Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • V. Kleff
    • 1
  • I. Rattmann
    • 1
  • C. Ludwig
    • 1
  • M. Flasshove
    • 1
  • T. Moritz
    • 1
  • B. Opalka
    • 1
  1. 1.Dept. of Internal Medicine (Cancer Research)University of Duisburg-Essen, Medical, SchoolEssenGermany

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