Abstract
Sodium butyrate (NaBu) can enhance the expression of foreign protein in recombinant Chinese hamster ovary (rCHO) cells, but it can also inhibit cell growth and induce cellular apoptosis. Thus, the beneficial effect of using a higher concentration of NaBu on foreign protein expression is compromised by its cytotoxic effect on cell growth. To overcome this cytotoxic effect of NaBu, we down-regulated caspase-3 by expressing caspase-3 siRNA. However, it was not sufficient for the inhibition of apoptotic cell death induced by NaBu. When subjected to NaBu treatment, the rCHO cells expressing caspase-3 siRNAs compensated for the lack of caspase-3 by increase of active caspase-7 level.
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Sung, Y.H., Park, S.H., Kim, S.H., Lee, G.M. (2007). Influence of Down-regulation of Caspase-3 and CASPASE-7 by Sirnas on Sodium Butyrate-induced Apoptotic Cell Death of Chinese Hamster Ovary Cells Producing Thrombopoietin. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_40
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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