Abstract
During process development in small-scale bioreactors, different materials used in the bioreactors were found to have a negative effect on cell growth and viability of HEK293 cells grown in protein-free medium. A variety of materials were tested including: stainless steel 316, Duran glass, ethylene propylene diene monomer (EPDM), silicone, C-flex, Viton, and low density polyethylene (LDPE). The most detrimental effect on cell growth and viability was seen with EPDM. Compared to the polystyrene control stainless steel and glass decreased cell growth while Viton, LDPE, and silicone showed the highest biocompatibility.
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References
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Aizawa, P., Karlsson, G., Benemar, C., Segl, V., Martinelle, K., Lindner, E. (2007). Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_23
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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