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Cultivation of Vero Cells on Microporous and Macroporous Microcarriers

  • Marta Cristina O. Souza
  • Marcos S. Freire
  • Leda R. Castilho

Abstract

The manufacture of immunobiologicals, such as viral vaccines, is largely based on animal cell technology. Large-scale production of viral antigens depends on efficient cell proliferation and virus replication, both in terms of quality and quantity. This can be achieved by choosing adequate culture conditions and cultivation systems. Microcarriers are a tool to provide a large adhesion area in homogeneous bioreactor systems, allowing high densities of anchorage-dependent cells to be obtained. The aim of the present work was to study the use of different microcarriers and culture conditions to obtain highdensity Vero cell cultures for the production of viral antigens.

Keywords

Vero Cell Viral Antigen Rabies Virus Agitation Rate Maximum Cell Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Yokomizo, A.Y., Antoniazzi, M.M., Galdino, P.L., Jorge, S.A.C., and Pereira, A.A, 2004, Rabies virus production in high Vero cell density cultures on macroporous microcarriers, Biotechnol. Bioeng. 85: 506–515.CrossRefGoogle Scholar
  2. Butler, M., Burgener, A., Patrick, M., Berry, M., Huzel, N., Barnabé, N., and Coombs, K., 2000, Application of a serum-free medium for the growth of Vero cells and the production of reovirus, Biotechnol. Prog. 16: 854–858.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Marta Cristina O. Souza
    • 1
  • Marcos S. Freire
    • 2
  • Leda R. Castilho
    • 1
  1. 1.Federal University of Rio de Janeiro - UFRJ/COPPE - Chemical Engineering ProgramRio de JaneiroBrazil
  2. 2.Oswaldo Cruz Foundation - FIOCRUZ - Bio-ManguinhosRio de JaneiroBrazil

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