Today, tomato yellow leaf curl disease has become the limiting factor for tomato production in many tropical and subtropical regions of the world. This disease is induced by a number of begomoviruses, the type member being Tomato yellow leaf curl virus (TYLCV), transmitted by the whitefly Bemisia tabaci (Gennadius), whose severe population outbreaks are usually associated with high incidence of the disease. Control measures in infected areas usually rely on seclusion of the whitefly vector, mainly through multiple applications of insecticides or physical barriers (Antignus & Cohen, 1994; Hilje et al., 2001; Palumbo et al., 2001; Polston & Anderson, 1997). Due to the large populations of whiteflies, and their ability to develop pesticide resistance, vector seclusion is not an ideal way of fighting the spread and damage induced by TYLCV. Hence, development of genetic resistance in the tomato host is the best solution for any virus problem, and especially for whitefly-transmitted viruses such as TYLCV, since it requires no chemical input and/or plant seclusion and may be stable and longlasting. Thus, the best way to reduce TYLCV spread is by breeding tomatoes resistant or tolerant to the virus (Lapidot & Friedmann, 2002; Morales, 2001; Pico et al., 1996).
Wild tomato species have been screened for their response to the virus and a number of TYLCV-resistant accessions identified, because no resistance has been found in the domesticated tomato (Solanum lycopersicum) (Lapidot & Friedmann, 2002; Nakhla & Maxwell, 1998; Pico et al., 1996). Thus, breeding programs have been based on the transfer of resistance genes from accessions of wild origin into the cultivated tomato. Progress in the breeding for TYLCV resistance has been slow, due in part to the complex genetics of the resistance and the presence of interspecific barriers between the wild and domesticated tomato species. The lack of an accurate and reliable mass inoculation and selection system has also slowed breeding programs. Since TYLCV is not transmitted mechanically, it is essential that inoculation protocols be developed using whiteflies, which can ensure 100% infection rate, and a standardized (as much as possible) inoculum pressure.
To succeed in a program aimed at developing resistance to TYLCV, a number of issues must be addressed: development of inoculation protocols, screening for resistant genotypes, development of a symptom-severity scale, inheritance of resistance, and determination of the effect a resistant host may have on virus epidemiology. This chapter focuses on aspects of screening for TYLCV resistance using whitefly-mediated transmission.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abou Jawdah, Y., Shebaro, W. A., & Soubra, K. H. (1995). Detection of tomato yellow leaf curl geminivirus (TYLCV) by a digoxigenin-labelled DNA probe. Phytopathol. Mediterr. 34, 52–57.
Antignus, Y. & Cohen, S. (1994). Complete nucleotide sequence of an infectious clone of a mild isolate of tomato yellow leaf curl virus (TYLCV). Phytopathology 84, 707–712.
Bellotti, A. C. & Arias, B. (2001). Host plant resistance to whiteflies with emphasis on cassava as a case study. Crop Prot. 20, 813–824.
Brown, J. K., Frohlich, D. R., & Rosell, R. C. (1995). The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annu. Rev. Entomol. 40, 511–669.
Byrne, D. N. & Bellows, T. S. (1991). Whitefly biology. Annu. Rev. Entomol. 36, 431–457.
Cohen, S. & Harpaz, I. (1964). Periodic rather than continual acquisition of a new tomato virus by its vector, the tobacco whitefly (Bemisia tabaci Gennadius). Entomol. Exp. Appl. 7, 155–166.
Cohen, S., Kern, J., Harpaz, I., & Ben Joseph, R. (1988). Epidemiological studies of the tomato yellow leaf curl virus (TYLCV) in the Jordan Valley, Israel. Phytoparasitica 16, 259–270.
Czosnek, H., Kheyr-Pour, A., Gronenborn, B., Remetz, E., Zeidan, M., Altman, A., Rabinowitch, H. D., Vidavsky, S., Kedar, N., Gafni, Y., & Zamir, D. (1993). Replication of tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf-disks from selected tomato genotypes. Plant Mol. Biol. 22, 995–1005.
Fargette, D., Leslie, M., & Harrison, B. D. (1996). Serological studies on the accumulation and localisation of three tomato leaf curl geminiviruses in resistant and susceptible Lycopersicon species and tomato cultivars. Ann. Appl. Biol. 128, 317–328.
Friedmann, M., Lapidot, M., Cohen, S., & Pilowsky, M. (1998). A novel source of resistance to tomato yellow leaf curl virus exhibiting a symptomless reaction to viral infection. J. Am. Soc. Hortic. Sci. 123, 1004–1007.
Grimsley, N., Hohn, B., Hohn, T., & Walden, R. (1986). “Agroinfection”, an alternative route for viral infection of plants by using the Ti plasmid. Proc. Natl. Acad. Sci. USA 83, 3282–3286.
Grimsley, N., Hohn, T., Davis, J. W., & Hohn, B. (1987). Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325, 177–179.
Hilje, L., Costa, H. S., & Stansly, P. A. (2001). Cultural practices for managing Bemisia tabaci and associated viral diseases. Crop Prot. 20, 801–812.
Jones, D. R. (2003). Plant viruses transmitted by whiteflies. Eur. J. Plant Pathol. 109, 195–219.
Kasrawi, M. A., Suwwan, M. A., & Mansour, A. (1988). Sources of resistance to tomato-yellow-leafcurl virus TYLCV in Lycopersicon species. Euphytica 37, 61–64.
Kheyr-Pour, A., Gronenborn, B., & Czosnek, H. (1994). Agroinoculation of tomato yellow leaf curl virus (TYLCV) overcomes the virus resistance of wild Lycopersicon species. Plant Breed. 112, 228–233.
Lapidot, M. & Friedmann, M. (2002). Breeding for resistance to whitefly-transmitted geminiviruses. Ann. Appl. Biol. 140, 109–127.
Lapidot, M. & Polston, J. E. (2006). Resistance to Tomato yellow leaf curl virus in tomato. In G. Lobensteine & J. P. Carr (Eds.), Natural Resistance Mechanisms of Plants to Viruses. The Netherlands: Springer, pp. 503–520.
Lapidot, M., Friedmann, M., Lachman, O., Yehezkel, A., Nahon, S., Cohen, S., & Pilowsky, M. (1997). Comparison of resistance level to tomato yellow leaf curl virus among commercial cultivars and breeding lines. Plant Dis. 81, 1425–1428.
Lapidot, M., Goldray, O., Ben Joseph, R., Cohen, S., Friedmann, M., Shlomo, A., Nahon, S., Chen, L., & Pilowsky, M. (2000). Breeding tomatoes for resistance to tomato yellow leaf curl begomovirus. Bull. OEPP/EPPO 30, 317–321.
Lapidot, M., Friedmann, M., Pilowsky, M., Ben Joseph, R., & Cohen, S. (2001). Effect of host plant resistance to Tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector. Phytopathology 91, 1209–1213.
Lapidot, M., Ben Joseph, R., Cohen, L., Machbash, Z., & Levy, D. (2006). Development of a scale for evaluation of Tomato yellow leaf curl virus-resistance level in tomato plants. Phytopathology 96, 1404–1408.
Makkouk, K. M., Shehab, S., & Majdalani, S. E. (1979). Tomato yellow leaf curl incidence yield losses and transmission in Lebanon. Phytopathologische Zeitschrift (J. Phytopathol.) 96, 263–267.
Morales, F. J. (2001). Conventional breeding for resistance to Bemisia tabaci-transmitted geminiviruses. Crop Prot. 20, 825–834.
Nakhla, M. K. & Maxwell, D. P. (1998). Epidemiology and management of tomato yellow leaf curl virus. In A. Hadidi, R. K. Khetarpal, H. Koganezawa (Eds.), Plant Virus Disease Control. St. Paul, MN: APS Press, pp. 565–583.
Palumbo, J. C., Horowitz, A. R., & Prabhaker, N. (2001). Insecticidal control and resistance management for Bemisia tabaci. Crop Prot. 20, 739–766. Perring, T. M. (2001). The Bemisia tabaci species complex. Crop Prot. 20, 725–737.
Perring, T. M., Cooper, A. D., Rodriguez, R. J., Farrar, C. A., & Bellows, T. S. (1993). Identification of a whitefly species by genomic and behavioural studies. Science 259, 74–77.
Pico, B., Diez, M. J., & Nuez, F. (1996). Viral diseases causing the greatest economic losses to the tomato crop. II: the tomato yellow leaf curl virus—a review. Sci. Hortic. 67, 151–196.
Pico, B., Diez, M., & Nuez, F. (1998). Evaluation of whitefly-mediated inoculation techniques to screen Lycopersicon esculentum and wild relatives for resistance to tomato yellow leaf curl virus. Euphytica 101, 259–271.
Pico, B., Ferriol, M., Diez, M. J., & Vinals, F. N. (2001). Agroinoculation methods to screen wild Lycopersicon for resistance to Tomato yellow leaf curl virus. J. Plant Pathol. 83, 215–220.
Polston, J. E. & Anderson, P. K. (1997). The emergence of whitefly-transmitted geminiviruses in tomato in the western hemisphere. Plant Dis. 81, 1358–1369.
Schuster, D. J., Muller, T. F., Kring, J. B., & Price, J. F. (1990). Relationship of the sweetpotato whitefly to a new tomato fruit disorder in Florida. HortScience 25, 1618–1620.
Segev, L., Cohen, L., & Lapidot, M. (2004). A tomato yellow leaf curl virus-resistant tomato line, TY-172, inhibits viral replication but not viral translocation. 4th International Geminivirus Symposium. Cape Town, South Africa, February 16–20, 2004.
Stenger, D. C., Revington, G. N., Stevenson, M. C., & Bisaro, D. M. (1991). Replication release of geminivirus genomes from tandemly repeated copies: evidence for rolling-circle replication of a plant viral DNA. Proc. Natl. Acad. Sci. USA 88, 8029–8033.
Vidavsky, F., Leviatov, S., Milo, J., Rabinowitch, H. D., Kedar, N., & Czosnek, H. (1998). Response of tolerant breeding lines of tomato, Lycopersicon esculentum, originating from three different sources (L. peruvianum, L. pimpinellifolium and L. chilense) to early controlled inoculation by tomato yellow leaf curl virus (TYLCV). Plant Breed. 117, 165–169.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this chapter
Cite this chapter
Lapidot, M. (2007). Screening for TYLCV-Resistance Plants using Whitefly-Mediated Inoculation. In: Czosnek, H. (eds) Tomato Yellow Leaf Curl Virus Disease. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4769-5_19
Download citation
DOI: https://doi.org/10.1007/978-1-4020-4769-5_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-4768-8
Online ISBN: 978-1-4020-4769-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)