Abstract
The X-ray crystal structure of cyanobacterial Photosystem I (PS I) has been solved to atomic resolution (PDB entry 1JB0). It provides a structural model for the subunits PsaC, PsaD, and PsaE that comprise the stromal ridge of PS I. Independently, the three-dimensional solution structures of unbound, recombinant PsaC (PDB entry 1K0T) and PsaE (PDB entries 1PSF, 1QP2, and 1GXI) have been solved using NMR spectroscopy.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adman ET, Sieker LC and Jensen LH (1973) The structure of a bacterial ferredoxin. J Biol Chem 248: 3987–3996
Adman ET, Siefker LC and Jensen LH (1976) Structure of Peptococcus aerogenes ferredoxin. Refinement at 2 Å resolution. J Biol Chem 251: 3801–3806
Antonkine ML, Bentrop D, Bertini I, Luchinat C, Shen GZ, Bryant DA, Stehlik D and Golbeck JH (2000) Paramagnetic 1H NMR spectroscopy of the reduced, unbound Photosystem I subunit PsaC: sequence-specific assignment of contact-shifted resonances and identification of mixed- and equal-valence Fe-Fe pairs in [4Fe-4S] centers FA − and FB −. J Biol Inorg Chem 5: 381–392
Antonkine ML, Liu GH, Bentrop D, Bryant DA, Bertini I, Luchinat C, Golbeck JH and Stehlik D (2002) Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I. J Biol Inorg Chem 7: 461–472
Antonkine ML, Jordan P, Fromme P, Krauß N, Golbeck JH and Stehlik D (2003) Assembly of protein subunits within the stromal ridge of Photosystem I. Structural changes between unbound and sequentially PS I-bound polypeptides and correlated changes of the magnetic properties of the terminal iron sulfur clusters. J Mol Biol 327: 671–697
Aono S, Bentrop D, Bertini I, Cosenza G and Luchinat C (1998a) Solution structure of an artificial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin. Eur J Biochem 258: 502–514
Aono S, Bentrop D, Bertini I, Donaire A, Luchinat C, Niikura Y and Rosato A (1998b) Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy. Biochemistry 37: 9812–9826
Barth P, Savarin P, Gilquin B, Lagoutte B and Ochsenbein F (2002) Solution NMR structure and backbone dynamics of the PsaE subunit of Photosystem I from Synechocystis sp. PCC 6803. Biochemistry 41: 13902–13914
Ben-Shem A, Frolow F and Nelson N (2003) Crystal structure of plant Photosystem I. Nature 426: 630–635
Bentrop D, Bertini I, Luchinat C, Nitschke W and Mühlenhoff U (1997) Characterization of the unbound 2[Fe4S4] ferredoxin-like Photosystem I subunit PsaC from the cyanobacterium Synechococcus elongatus. Biochemistry 36: 13629–13637
Bentrop D, Capozzi F and Luchinat C (2001) Iron-sulfur proteins. In: Bertini I, Sigel A and Sigel H (eds) Handbook on Metalloproteins, pp 357–460. Marcel Dekker, New York
Bertini I, Donaire A, Feinberg BA, Luchinat C, Piccioli M and Yuan HP (1995) Solution structure of the oxidized 2[4Fe-4S] ferredoxin from Clostridium pasteurianum. Eur J Biochem 232: 192–205
Cantrell A and Bryant DA (1987) Molecular-cloning and nucleotide-sequence of the psaA and psaB genes of the cyanobacterium Synechococcus sp. PCC 7002. Plant Mol Biol 9: 453–468
Chamorovsky SK and Cammack R (1982) Direct determination of the midpoint potential of the acceptor X in chloroplast Photosystem I by electrochemical reduction and ESR spectroscopy. Photobiochem Photobiophys 4: 195–200
Chitnis VP, Jung YS, Albee L, Golbeck JH and Chitnis PR (1996) Mutational analysis of Photosystem I polypeptides –role of PsaD and the Lysyl 106 residue in the reductase activity of photosystem I. J Biol Chem 271: 11772–11780
Dauter Z, Wilson KS, Sieker LC, Meyer J and Moulis JM (1997) Atomic resolution (0.94 Å) structure of Clostridium acidi urici ferredoxin. Detailed geometry of [4Fe-4S] clusters in a protein. Biochemistry 36: 16065–16073
Diaz-Moreno I, Diaz-Quintana A, Molina-Heredia FP, Nieto PM, Hansson O, De la Rosa MA and Karlsson BG (2005) NMR analysis of the transient complex between membrane Photosystem I and soluble cytochrome c6. J Biol Chem 280: 7925–7931
Diaz-Quintana A, Leibl W, Bottin H and Sétif P (1998) Electron transfer in Photosystem I reaction centers follows a linear pathway in which iron-sulfur cluster FB is the immediate electron donor to soluble ferredoxin. Biochemistry 37: 3429–3439
Duee ED, Fanchon E, Vicat J, Sieker LC, Meyer J and Moulis JM (1994) Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acid urici at 1.84 Å resolution. J Mol Biol 243: 683–695
Falzone CJ, Kao YH, Zhao JD, Bryant DA and Lecomte JTJ (1994) Three-dimensional solution structure of PsaE from the cyanobacterium Synechococcus sp. strain PCC 7002, a Photosystem I protein that shows structural homology with SH3 domains. Biochemistry 33: 6052–6062
Fiaux J, Bertelsen EB, Horwich AL and Wuthrich K (2002) NMR analysis of a 900K GroEL-GroES complex. Nature 418: 207–211
Fischer N, Hippler M, Sétif P, Jacquot JP and Rochaix JD (1998) The PsaC subunit of photosystem I provides an essential lysine residue for fast electron transfer to ferredoxin. EMBO J 17: 849–858
Fischer N, Sétif P and Rochaix JD (1999) Site-directed mutagenesis of the PsaC subunit of Photosystem I. FB is the cluster interacting with soluble ferredoxin. J Biol Chem 274: 23333–23340
Fish L, Kück U and Bogorad L (1985) Two partially adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a protein complex of Photosystem I. J Biol Chem 260: 1413–1421
Fromme P, Jordan P and Krauß N (2001) Structure of Photosystem I. Biochim Biophys Acta 1507: 5–31
Golbeck JH (1994) Photosystem I in cyanobacteria. In: Bryant DA (ed) The Molecular Biology of Cyanobacteria, pp 179–220. Kluwer Academic Publishers, The Netherlands
Golbeck JH (1999) A comparative analysis of the spin state distribution of in vitro and in vivo mutants of PsaC –a biochemical argument for the sequence of electron transfer in Photosystem I as FX → FA → FB → ferredoxin/flavodoxin. Photosynth Res 61: 107–144
Golbeck JH and Bryant DA (1991) Photosystem I. In: Lee CP (ed) Current Topics in Bioenergetics, Vol 16, pp 83–177. Academic Press, Inc., San Diego
Golbeck JH, Parrett KG, Mehari T, Jones KL and Brand JJ (1988) Isolation of the intact Photosystem I reaction center core containing P700 and iron-sulfur center FX. FEBS Lett 228: 268–272
Heinnickel M, Shen G, Agalarov R and Golbeck J (2005) Resolution and reconstitution of a bound Fe-S protein from the photosynthetic reaction center of Heliobacterium modesticaldum. Biochemistry 44: 9950–9960
Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three-dimensional structure of cyanobacterial Photosystem I at 2.5 Å resolution. Nature 411: 909–917
Jung YS, Yu L and Golbeck JH (1995) Reconstitution of iron-sulfur center FB results in complete restoration of NADP+ photoreduction in Hg-treated Photosystem I complexes from Synechococcus sp PCC 6301. Photosynth Res 46: 249–255
Ke B, Dolan E, Sugahara K, Hawkridge FM, Demeter S and Shaw ER (1977) Electrochemical and kinetic evidence for a transient electron acceptor in the photochemical charge separation in Photosystem I. In: Photosynthetic Organelles [Plant Cell Physiol (Special Issue no. 3)] pp 187–199
Klukas O, Schubert WD, Jordan P, Krauß N, Fromme P, Witt HT and Saenger W (1999) Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE. J Biol Chem 274: 7351–7360
Koradi R, Billeter M and Wuthrich K (1996) MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph 14: 29–32, 51–55
Lakshmi KV, Jung YS, Golbeck JH and Brudvig GW (1999) Location of the iron-sulfur clusters FA and FB in Photosystem I: an electron paramagnetic resonance study of spin relaxation enhancement of P700+. Biochemistry 38: 13210–13215
Li N, Zhao J, Warren PV, Warden JT, Bryant DA and Golbeck JH (1991a) PsaD is required for the stable binding of PsaC to the Photosystem I core protein of Synechococcus sp. PCC 6301. Biochemistry 30: 7863–7872
Li N, Warren P, Golbeck J, Frank G, Zuber H and Bryant D (1991b) Polypeptide composition of the Photosystem-I complex and the Photosystem-I core protein from Synechococcus sp. PCC 6301. Biochim Biophys Acta 1059: 215–225
Locher KP, Hans M, Yeh AP, Schmid B, Buckel W and Rees DC (2001) Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A. J Mol Biol 307: 297–308
Lovenberg W, Rabinowitz JC and Buchanan BB (1963) Studies on chemical nature of clostridial ferredoxin. J Biol Chem 238: 3899–3913
Mamedov MD, Gourovskaya KN, Vassiliev IR, Golbeck JH and Sememov AY (1998) Electrogenicity accompanies photoreduction of the iron-sulfur clusters FA and FB in Photosystem I. FEBS Lett 431: 219–223
Mayer KL, Shen GZ, Bryant DA, Lecomte JTJ and Falzone CJ (1999) The solution structure of Photosystem I accessory protein E from the cyanobacterium Nostoc sp. strain PCC 8009. Biochemistry 38: 13736–13746
McDermott AE, Yachandra VK, Guiles RD, Sauer K, Klein MP, Parrett KG and Golbeck JH (1989) EXAFS structural study of FX, the low-potential Fe-S center in Photosystem I. Biochemistry 28: 8056–8059
Mehari T, Parrett KG, Warren PV and Golbeck JH (1991) Reconstitution of the iron-sulfur clusters in the isolated FA/FB protein: EPR spectral characterization of same-species and cross-species Photosystem I complexes. Biochim Biophys Acta 1056: 139–148
Moulis JM, Sieker LC, Wilson KS and Dauter Z (1996) Crystal structure of the 2[4Fe-4S] ferredoxin from Chromatium vinosum: evolutionary and mechanistic inferences for [3/4Fe-4S] ferredoxins. Protein Sci 5: 1765–1775
Parrett KG, Mehari T, Warren PG and Golbeck JH (1989) Purification and properties of the intact P700 and FX-containing Photosystem I core protein. Biochim Biophys Acta 973: 324–332
Pervushin K, Riek R, Wider G and Wuthrich K (1997) Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci USA 94: 12366–12371
Petrouleas V, Brand JJ, Parrett KG and Golbeck JH (1989) A Mössbauer analysis of the low-potential iron-sulfur center in Photosystem I –spectroscopic evidence that FX is a 4Fe-4S cluster. Biochemistry 28: 8980–8983
Rousseau F, Sétif P and Lagoutte B (1993) Evidence for the involvement of PSI-E subunit in the reduction of ferredoxin by Photosystem I. EMBO J 12: 1755–1765
Saenger W, Jordan P and Krauß N (2002) The assembly of protein subunits and cofactors in Photosystem I. Curr Opin Struct Biol 12: 244–254
Schlessman JL, Woo D, Joshua-Tor L, Howard JB and Rees DC (1998) Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum. J Mol Biol 280: 669–685
Sétif P (2001) Ferredoxin and flavodoxin reduction by Photosystem I. Biochim Biophys Acta 1507: 161–179
Shinkarev VP, Vassiliev IR and Golbeck JH (2000) A kinetic assessment of the sequence of electron transfer from FX to FA and further to FB in Photosystem I: the value of the equilibrium constant between FX and FA. Biophys J 78: 363–372
Sticht H and Rosch P (1998) The structure of iron-sulfur proteins. Prof Phys Mol Biol 70: 95–136
Stout CD (1993) Crystal structures of oxidized and reduced Azotobacter vinelandii ferredoxin at pH 8 and pH 6. J Biol Chem 268: 25920–25927
Tranqui D and Jesior JC (1995) Structure of the ferredoxin from Clostridium acidi urici –model at 1.8 Å resolution. Acta Crystallogr D Biol Crystallogr 51: 155–159
Unciuleac M, Boll M, Warkentin E and Ermler U (2004) Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin. Acta Crystallogr D Biol Crystallogr 60: 388–391
Vassiliev IR, Jung YS, Yang F and Golbeck JH (1998) PsaC subunit of Photosystem I is oriented with iron-sulfur cluster FB as the immediate electron donor to ferredoxin and flavodoxin. Biophys J 74: 2029–2035
Vassiliev IR, Antonkine ML and Golbeck JH (2001) Iron-sulfur clusters in type I reaction centers. Biochim Biophys Acta 1507: 139–160
Xia ZC, Broadhurst RW, Laue ED, Bryant DA, Golbeck JH and Bendall DS (1998) Structure and properties in solution of PsaD, an extrinsic polypeptide of Photosystem I. Eur J Biochem 255: 309–316
Xu Q, Jung YS, Chitnis VP, Guikema JA, Golbeck JH and Chitnis PR (1994) Mutational analysis of Photosystem I polypeptides in Synechocystis sp. PCC 6803 –subunit requirements for reduction of NADP+ mediated by ferredoxin and flavodoxin. J Biol Chem 269: 21512–21518
Yu JP, Smart LB, Jung YS, Golbeck J and Mcintosh L (1995) Absence of PsaC subunit allows assembly of Photosystem I core but prevents the binding of PsaD and PsaE in Synechocystis sp PCC 6803. Plant Mol Biol 29: 331–342
Zhao J, Warren PV, Li N, Bryant DA and Golbeck JH (1990) Reconstitution of electron transport in Photosystem I with PsaC and PsaD proteins expressed in Escherichia coli. FEBS Lett 276: 175–180
Zhao J, Li N, Warren P, Golbeck J and Bryant D (1992) Site-directed conversion of a cysteine to aspartate leads to the assembly of a [3Fe-4S] cluster in PsaC of Photosystem I –the photoreduction of FA is independent of FB. Biochemistry 31: 5093–5099
Zhao J, Snyder WB, Mühlenhoff U, Rhiel E, Warren PV, Golbeck JH and Bryant DA (1993) Cloning and characterization of the psaE gene of the cyanobacterium Synechococcus sp. PCC 7002 –characterization of a psaE mutant and overproduction of the protein in Escherichia coli. Mol Microbiol 9: 183–194
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer
About this chapter
Cite this chapter
Antonkine, M.L., Golbeck, J.H. (2006). Molecular Interactions of the Stromal Subunit PsaC with the PsaA/PsaB Heterodimer. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-4020-4256-0_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-4255-3
Online ISBN: 978-1-4020-4256-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)