Structure, Function, and Regulation of Plant Photosystem I

  • Nathan Nelson
  • Adam Ben-Shem
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 24)


The recently determined structure of plant photosystem I (PS I) provides the first relatively high-resolution structural model of a supercomplex containing a reaction center and its peripheral antenna. The peripheral antenna of PS I (LHCI) is composed of four gene products (Lhca1–4) that are unique among the chlorophyll a/b binding proteins in their pronounced long-wavelength absorbance and their assembly into dimers. We describe some of the structural features responsible for the unique properties of LHCI and its interaction with the reaction center. The possible architecture of the docking sites for plastocyanin, ferredoxin, ferredoxin:NADP+ reductase, and LHCII are discussed.


Transmembrane Helix Detergent Concentration Electron Crystallography Peripheral Antenna Plant Photosystem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bailey S, Walters RG, Jansson S and Horton P (2001) Acclimation of Arabidopsis thaliana to the light environment: the existence of separate low light and high light responses. Planta 213: 794–801PubMedCrossRefGoogle Scholar
  2. Barber J (2002) Photosystem II: a multisubunit membrane protein that oxidises water. Curr Opin Struct Biol 12: 523–530PubMedCrossRefGoogle Scholar
  3. Bengis C and Nelson N (1975) Purification and properties of the photosystem I reaction center from chloroplasts. J Biol Chem 250: 2783–2788PubMedGoogle Scholar
  4. Bengis C and Nelson N (1977) Subunit structure of chloroplast photosystem I reaction center. J Biol Chem 252: 4564–4569PubMedGoogle Scholar
  5. Ben-Shem A, Nelson N and Frolow F (2003a) Crystallization and initial X-ray diffraction studies of higher plant photosystem I. Acta Crystallogr D 59: 1824–1827CrossRefGoogle Scholar
  6. Ben-Shem A, Frolow F and Nelson N (2003b) The crystal structure of plant photosystem I. Nature 426: 630–635CrossRefGoogle Scholar
  7. Ben-Shem A, Frolow F and Nelson N (2004a) Evolution of Photosystem I –from symmetry through pseudosymmetry to asymmetry. FEBS Lett 564: 274–280CrossRefGoogle Scholar
  8. Ben-Shem A, Frolow F and Nelson N (2004b) Light-harvesting features revealed by the structure of plant photosystem I. Photosynth Res 81: 239–250CrossRefGoogle Scholar
  9. Chitnis VP, Xu Q, Yu L, Golbeck JH, Nakamoto H, Xie DL and Chitnis PR (1993) Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem 268: 11678–11684PubMedGoogle Scholar
  10. Chow WS, Melis A and Anderson JM (1990) Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci USA 87: 7502–7506PubMedCrossRefGoogle Scholar
  11. Croce R, Morosinotto T, Castelletti S, Breton J and Bassi R (2002) The Lhca antenna complexes of higher plants photosystem I. Biochim Biophys Acta 1556: 29–40PubMedCrossRefGoogle Scholar
  12. Durnford DG, Deane JA, Tan S, McFadden GI, Gantt E and Green BR (1999) A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution. J Mol Evol 48: 59–68PubMedCrossRefGoogle Scholar
  13. Ferreira KN, Iverson TM, Maghlaoui K, Barber J and Iwata S (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303: 1831–1838PubMedCrossRefGoogle Scholar
  14. Ganeteg U, Strand A, Gustafsson P and Jansson S (2001) The properties of the chlorophyll a/b-binding proteins Lhca2 and Lhca3 studied in vivo using antisense inhibition. Plant Physiol 127: 150–158PubMedCrossRefGoogle Scholar
  15. Jansson S, Andersen B and Scheller HV (1996) Nearest-neighbor analysis of higher-plant photosystem I holocomplex. Plant Physiol 112: 409–420PubMedCrossRefGoogle Scholar
  16. Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution. Nature 411: 909–917PubMedCrossRefGoogle Scholar
  17. Kuhlbrandt W, Wang DN and Fujiyoshi Y (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367: 614–621PubMedCrossRefGoogle Scholar
  18. Kurisu G, Kusunoki M, Katoh E, Yamazaki T, Teshima K, Onda Y, Kimata-Ariga Y and Hase T (2001) Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP(+) reductase. Nat Struct Biol 8: 117–121PubMedCrossRefGoogle Scholar
  19. Liu Z, Yan H, Wang K, Kuang T, Zhang J, Gui L, An X and Chang W (2004) Crystal structure of spinach major light-harvesting complex at 2.2 Å resolution. Nature 428: 287–292PubMedCrossRefGoogle Scholar
  20. Lunde CP, Jensen PE, Haldrup A, Knoetzel J and Scheller HV (2000) The PS I-H subunit of photosystem I is essential for state transitions in plant photosynthesis. Nature 408: 613–615PubMedCrossRefGoogle Scholar
  21. Morosinotto T, Castelletti S, Breton J, Bassi R and Croce R (2002) Mutation analysis of Lhca1 antenna complex low energy absorption forms originate from pigment–pigment interactions. J Biol Chem 277: 36253–36261PubMedCrossRefGoogle Scholar
  22. Mullet JE, Burke JJ and Arntzen CJ (1980) Chlorophyll proteins of photosystem I. Plant Physiol 65: 814–822PubMedGoogle Scholar
  23. Nelson N and Ben-Shem A (2002) Photosystem I reaction center: past and future. Photosynth Res 73: 193–206PubMedCrossRefGoogle Scholar
  24. Nelson N and Ben-Shem A (2004) The complex architecture of oxygenic photosynthesis. Nat Rev Mol Cell Biol 6, 818–818Google Scholar
  25. Park Y, Chow WS and Anderson JM (1997) Antenna size dependency of photoinactivation of photosystem II in light-acclimated pea leaves. Plant Physiol 115: 151–157PubMedCrossRefGoogle Scholar
  26. Scheller HV, Jensen PE, Haldrup A, Lunde C and Knoetzel J (2001) Role of subunits in eukaryotic Photosystem I. Biochim Biophys Acta 1507: 41–60PubMedCrossRefGoogle Scholar
  27. Trissl H-W and Wilhelm C (1993) Why do thylakoid membranes from higher plants form grana stacks? Trends Biochem Sci 18: 415–419PubMedCrossRefGoogle Scholar
  28. Xue Y, Okvist M, Hansson O and Young S (1998) Crystal structure of spinach plastocyanin at 1.7 Å resolution. Protein Sci 7: 2099–2105PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Nathan Nelson
    • 1
  • Adam Ben-Shem
    • 1
  1. 1.Department of Biochemistry, The George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael

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