Association of Photosystem I and Light-Harvesting Complex II during State Transitions

  • Egbert J. Boekema
  • Roman Kouřil
  • Jan P. Dekker
  • Poul Erik Jensen
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 24)


Green plant photosystem I (PS I) not only binds a chlorophyll a/b-binding, membrane-intrinsic antenna complex (LHCI) that is associated with the PS I core complex under almost all physiological conditions, but it can also transiently bind the major chlorophyll a/b-binding light-harvesting complex (LHCII), when the light conditions favor excitation of photosystem II (PS II) and the photosynthetic apparatus is in the so-called state 2. Recently, a low-resolution structure was obtained of a PS I–LHCII supercomplex from Arabidopsis thaliana. We describe here some of the structural features of this transient complex, and discuss the role of small PS I subunits that are involved in the binding of LHCII.We also discuss structural features of the PS I complex of the green algae Chlamydomonas reinhardtii, which has a larger LHCI antenna and shows a more pronounced difference between state 1 and state 2.


Thylakoid Membrane Protein Trimeric LHCII LHCII Complex Cyclic Electron Transfer Monomeric LHCII 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen JF (1992) Protein phosphorylation in regulation of photosynthesis. Biochim Biophys Acta 1098: 275–335PubMedCrossRefGoogle Scholar
  2. Allen JF and Forsberg J (2001) Molecular recognition in thylakoid structure and function. Trends Plant Sci 6: 317–326PubMedCrossRefGoogle Scholar
  3. Bassi R, Giacometti GM and Simpson DJ (1988) Changes in the organization of stroma membranes induced by in vivo state-1–state-2-transition. Biochim Biophys Acta 935: 152–165CrossRefGoogle Scholar
  4. Bellafiore S, Barneche F, Peltier G and Rochaix JD (2005) State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature 433: 892–895PubMedCrossRefGoogle Scholar
  5. Ben-Shem A and Nelson N (2005) System biology of photosystem I – formation of supercomplexes. In: Van der Est A and Bruce D (eds) Photosynthesis: Fundamental Aspects to Global Perspectives, pp 770–772. Alliance Communications Group, Lawrence, KansasGoogle Scholar
  6. Ben-Shem A, Frolow F and Nelson N (2003) Crystal structure of plant photosystem I. Nature 426: 630–635PubMedCrossRefGoogle Scholar
  7. Boekema EJ, van Breemen JFL, van Roon H and Dekker JP (2000) Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts. J Mol Biol 301: 1123–1133PubMedCrossRefGoogle Scholar
  8. Boekema EJ, Jensen PE, Schlodder E, van Breemen JFL, van Roon H, Scheller HV and Dekker JP (2001) Green plant photosystem I binds light-harvesting complex I on the side of the complex. Biochemistry 40: 1029–1036PubMedCrossRefGoogle Scholar
  9. Dekker JP and Boekema EJ (2005) Supramolecular organization of thylakoid membrane proteins in green plants. Biochim Biophys Acta 1706: 12–39PubMedCrossRefGoogle Scholar
  10. Delosme R, Olive J and Wollman F-A (1996) Changes in light energy distribution upon state transitions: an in vivo photoacoustic study of the wild type and photosynthetic mutants from Chlamydomonas reinhardtii. Biochim Biophys Acta 1273: 150–158CrossRefGoogle Scholar
  11. Depège N, Bellafiore S and Rochaix JD (2003) Role of chloroplast protein kinase Stt7 in LHCII phosphorylation and state transition in Chlamydomonas. Science 299: 1572–1575PubMedCrossRefGoogle Scholar
  12. Finazzi G, Rappaport F, Furia A, Fleischmann M, Rochaix J-D, Zito F and Forti G (2002) Involvement of state transitions in the switch between linear and cyclic electron flow in Chlamydomonas reinhardtii. EMBO Rep 3: 280–285PubMedCrossRefGoogle Scholar
  13. Garab G, Cseh Z, Kovacs L, Rajagopal S, Varkonyi Z, Wentworth M, Mustardy L, Der A, Ruban AV, Papp E, Holzenburg A and Horton P (2002) Light-induced trimer to monomer transition in the main light-harvesting antenna complex of plants: thermo-optic mechanism. Biochemistry 41: 15141–15149CrossRefGoogle Scholar
  14. Germano M, Yakushevska AE, Keegstra W, van Gorkom HJ, Dekker JP and Boekema EJ (2002) Supramolecular organization of photosystem I and light-harvesting complex I in Chlamydomonas reinhardtii. FEBS Lett 525: 121–125PubMedCrossRefGoogle Scholar
  15. Haldrup A, Jensen PE, Lunde C and Scheller HV (2001) Balance of power: a view of the mechanism of photosynthetic state transitions. Trends Plant Sci 6: 301–305PubMedCrossRefGoogle Scholar
  16. Jansson S (1994) The light-harvesting chlorophyll a/b proteins. Biochim Biophys Acta 1184: 1–19PubMedCrossRefGoogle Scholar
  17. Jensen PE, Gilpin M, Knoetzel J and Scheller HV (2000) The PSI-K subunit of photosystem I is involved in the interaction between light-harvesting complex I and the photosystem I reaction center core. J Biol Chem 275: 24701–24708PubMedCrossRefGoogle Scholar
  18. Jensen PE, Haldrup A, Zhang S and Scheller HV (2004) The PSI-O subunit of plant photosystem I is involved in balancing the excitation pressure between the two photosystems. J Biol Chem 279: 24212–24217PubMedCrossRefGoogle Scholar
  19. Kargul J, Nield J and Barber J (2003) Three-dimensional reconstruction of a light-harvesting complex I-photosystem I (LHCI-PSI) supercomplex from the green alga Chlamydomonas reinhardtii –insights into light harvesting for PSI. J Biol Chem 278: 16135–16141PubMedCrossRefGoogle Scholar
  20. Khrouchtchova A, Hansson M, Paakkarinen V, Vainonen JP, Zhang S, Jensen PE, Scheller HV, Vener AV, Aro E-M and Haldrup A (2005) A previously found thylakoid membrane protein of 14 kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P. FEBS Lett 29: 4808–4812CrossRefGoogle Scholar
  21. Kouřil R, Zygadlo A, Arteni AA, de Wit CD, Dekker JP, Jensen PE, Scheller HV and Boekema EJ (2005) Structural characterization of a complex of photosystem I and light-harvesting complex II in Arabidopsis thaliana. Biochemistry 44: 10935–10940PubMedCrossRefGoogle Scholar
  22. Lunde C, Jensen PE, Haldrup A, Knoetzel J and Scheller HV (2000) The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis. Nature 408: 613–615PubMedCrossRefGoogle Scholar
  23. Lunde C, Jensen PE, Rosgaard L, Haldrup A, Gilpin MJ and Scheller HV (2003) Plants impaired in state transitions can to a large degree compensate for their defect. Plant Cell Physiol 44: 44–54PubMedCrossRefGoogle Scholar
  24. Nilsson A, Stys D, Drakenberg T, Spangfort MD, Forsén S and Allen JF (1997) Phosphorylation controls the three-dimensional structure of plant light-harvesting complex II. J Biol Chem 272: 18350–18357PubMedCrossRefGoogle Scholar
  25. Snyders S and Kohorn BD (1999) TAKs, thylakoid membrane protein kinases associated with energy transduction. J Biol Chem 274: 9137–9140PubMedCrossRefGoogle Scholar
  26. Snyders S and Kohorn BD (2001) Disruption of thylakoid-associated kinase 1 leads to alteration of light harvesting in Arabidopsis. J Biol Chem 276: 32169–32176PubMedCrossRefGoogle Scholar
  27. Takahashi Y, Yasui T-A, Stauber EJ and Hippler M (2004) Comparison of the subunit compositions of the PSI–LHCI supercomplex and the LHCI in the green alga Chlamydomonas reinhardtii. Biochemistry 43: 7816–7823PubMedCrossRefGoogle Scholar
  28. Vener AV, van Kan PJ, Rich PR, Ohad I and Andersson B (1997) Plastoquinol at the quinol oxidation site of reduced cytochrome bf mediates signal transduction between light and protein phosphorylation: thylakoid protein kinase deactivation by a single-turnover flash. Proc Natl Acad Sci USA 94: 1585–1590PubMedCrossRefGoogle Scholar
  29. Wollman FA (2001) State transitions reveal the dynamics and flexibility of the photosynthetic apparatus. EMBO J 20: 3623–3630PubMedCrossRefGoogle Scholar
  30. Yakushevska AE, Jensen PE, Keegstra W, van Roon H, Scheller HV, Boekema EJ and Dekker JP (2001) Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana. Eur J Biochem 268: 6020–6028PubMedCrossRefGoogle Scholar
  31. Zhang S and Scheller HV (2004) Light-harvesting complex II binds to several small subunits of photosystem I. J Biol Chem 279: 3180–3187PubMedCrossRefGoogle Scholar
  32. Zito F, Finazzi G, Delosme R, Nitschke W, Picot D and Wollman FA (1999) The Qo site of cytochrome b(6)f complexes controls the activation of the LHCII kinase. EMBO J 8: 2961–2969CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Egbert J. Boekema
    • 1
  • Roman Kouřil
    • 1
  • Jan P. Dekker
    • 2
  • Poul Erik Jensen
    • 3
  1. 1.Department of Biophysical Chemistry, GBBUniversity of GroningenGroningenThe Netherlands
  2. 2.Division of Physics and Astronomy, Faculty of SciencesVrije UniversiteitAmsterdamThe Netherlands
  3. 3.Plant Biochemistry Laboratory, Department of Plant BiologyThe Royal Veterinary and Agricultural UniversityFrederiksbergDenmark

Personalised recommendations