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Photosystem I pp 455-476 | Cite as

Electron Transfer From Ferredoxin and Flavodoxin to Ferredoxin:NADP+ Reductase

  • John K. Hurley
  • Gordon Tollin
  • Milagros Medina
  • Carlos Gómez-Moreno
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 24)

Abstract

An overview is presented of structure/function relationships in the interactions between the small electron transfer proteins ferredoxin (Fd) and flavodoxin (Fld) and the flavoprotein enzyme ferredoxin:NADP+ reductase (FNR), primarily emphasizing the proteins from the cyanobacterium, Anabaena, and the higher plant, spinach. Results are summarized from experiments utilizing rapid-reaction kinetic methods (stopped-flow spectrophotometry and laser flash photolysis) involving wild-type and site-specific mutants of these proteins, redox potential determinations, and X-ray crystallography, including the crystal structure of a Fd/FNR complex. These have provided detailed insights into the protein–protein recognition and electron transfer mechanisms utilized by these systems. Fd and Fld bind to FNR within a concave region of the FNR surface that contains the exposed dimethylbenzene ring of the FAD cofactor. In the Fd case, electron transfer between the iron–sulfur and flavin centers proceeds with a maximum rate constant of 5,500 sec−1 via a direct outer-sphere mechanism. Both electrostatic and hydrophobic interactions occur between the proteins, resulting in a precise surface complementarity.

Keywords

Spirulina Platensis Crystalline Complex Anabaena Variabilis Midpoint Redox Potential Isoalloxazine Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2006

Authors and Affiliations

  • John K. Hurley
    • 1
  • Gordon Tollin
    • 1
  • Milagros Medina
    • 2
  • Carlos Gómez-Moreno
    • 2
  1. 1.Department of Biochemistry and Molecular BiophysicsUniversity of ArizonaTucsonUSA
  2. 2.Departmento de Bioquímica y Biología Molecular y CelularUniversidad de ZaragozaZaragozaSpain

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