Abstract
High-field frequency EPR and multifrequency quantum beat studies of the electron transfer intermediates in Photosystem I reveal new details of structure and function that could not be obtained without the enhanced resolution, both spectral and temporal, and sensitivity of these advanced spectroscopic techniques. The results of careful measurements and analyses of the resolved g-tensors of the primary donor cation radical and excited triplet state show that their electronic structures differ from those of monomeric chlorophyll a. Multifrequency time-resolved EPR, which includes high-field EPR, has made it possible to determine the structure of the transient charge-separated state P+ 700A1 − and its orientation in the thylakoid membrane. High-field EPR studies are just beginning to have an impact on photosynthesis research, as the technique has only been widely accessible for the last few years. There are several important advantages of high-field EPR spectroscopy. First is the high g-tensor resolution.
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Thurnauer, M.C., Poluektov, O.G., Kothe, G. (2006). High-Field EPR Studies of Electron Transfer Intermediates in Photosystem I. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_22
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