Skip to main content
SpringerLink
Log in

FTIR Studies of the Primary Electron Donor, P700

  • Chapter
Photosystem I

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 24))

Abstract

Over the last two decades FTIR difference spectroscopy has emerged as a prominent technique to investigate the electronic structure and the bonding interactions of P700, the primary electron donor of photosystem I. In this chapter, the advances in the field during this period are reviewed and discussed in the light of the structural model of P700 derived from X-ray crystallography. The effect on the FTIR difference spectra of mutations of the axial ligands of the chlorophyll molecules in P700 as well as of amino acid residues in hydrogen bonding interaction with the carbonyl groups of P700 is analyzed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Breton J (2001) Fourier transform infrared spectroscopy of primary electron donors in type I photosynthetic reaction centers. Biochim Biophys Acta 1507: 180–193

    Article  PubMed  CAS  Google Scholar 

  • Breton J and Nabedryk E (1993) S0 → T1 infrared difference spectrum of the triplet state of the primary electron donor in Rb. sphaeroides photosynthetic reaction centers. Chem Phys Lett 213: 571–575

    Article  CAS  Google Scholar 

  • Breton J and Nabedryk E (1996) Protein-quinone interactions in the bacterial photosynthetic reaction center: light-induced FTIR difference spectroscopy of the quinone vibrations. Biochim Biophys Acta 1275: 84–90

    Article  Google Scholar 

  • Breton J, Nabedryk E and Parson WW (1992) A new electronic transition of the oxidized primary electron donor in bacterial reaction centers: a way to assess resonance interactions between the bacteriochlorophylls. Biochemistry 31: 7503–7510

    Article  PubMed  CAS  Google Scholar 

  • Breton J, Nabedryk E, Allen JP and Williams JC (1997) Electrostatic influence of QA reduction on the IR vibrational mode of the 10-a ester C=O of HA demonstrated by mutations at residues Glu L104 and Trp L100 in reaction centers from Rhodobacter sphaeroides. Biochemistry 36: 4515–4525

    Article  PubMed  CAS  Google Scholar 

  • Breton J, Nabedryk E and Leibl W (1999) FTIR study of the primary electron donor of photosystem I (P700) revealing delocalization of the charge in P700+ and localization of the triplet character in 3P700. Biochemistry 38: 11585–11592

    Article  PubMed  CAS  Google Scholar 

  • Breton J, Xu W, Diner BA and Chitnis P (2002) The two histidine axial ligands of the primary electron donor chlorophylls (P700) in photosystem I are similarly perturbed upon P700+ formation. Biochemistry 41: 11200–11210

    Article  PubMed  CAS  Google Scholar 

  • Breton J, Chitnis P and Pantelidou M (2005) Evidence for hydrogen bond formation to the PsaB chlorophyll of P700 in photosystem I mutants of Synechocystis sp. PCC 6803. Biochemistry 44: 5402–5408

    Article  PubMed  CAS  Google Scholar 

  • Fujiwara M and Tasumi M (1986) Metal-sensitive bands in the Raman and infrared spectra of intact and metal-substituted chlorophyll a. J Phys Chem 90: 5646–5650

    Article  CAS  Google Scholar 

  • Goldsmith JO, King B and Boxer SG (1996) Mg coordination by amino acid side chains is not required for assembly and function of the special pair in the photosynthetic reaction center. Biochemistry 35: 2421–2428

    Article  PubMed  CAS  Google Scholar 

  • Hamacher E, Kruip J, Rögner M and Mäntele W (1996) Characterization of the primary electron donor of photosystem I, P700, by electrochemistry and Fourier transform infrared (FTIR) difference spectroscopy. Spectrochim Acta A 52: 107–121

    Article  Google Scholar 

  • Hasegawa K, Ono T and Noguchi T (2002) Ab initio density functional theory calculations and vibrational analysis of zinc-bound 4-methylimidazole as a model of a histidine ligand in metalloenzymes. J Phys Chem A 106: 3377–3390

    Article  CAS  Google Scholar 

  • Hastings G (2001) Time-resolved step-scan Fourier transform infrared and visible absorption difference spectroscopy for the study of photosystem I. Appl Spectrosc 55: 894–900

    Article  CAS  Google Scholar 

  • Hastings G, Ramesh VM, Wang R, Sivakumar V and Weber A (2001) Primary donor photo-oxidation in photosystem I: a re-evaluation of (P700+ − P700) Fourier transform infrared difference spectra. Biochemistry 40: 12943–12949

    Article  PubMed  CAS  Google Scholar 

  • Iwaki M, Andrianambinintsoa S, Rich P and Breton J (2002) Attenuated total reflection Fourier transform infrared spectroscopy of redox transitions in photosynthetic reaction centers: comparison of perfusion- and light-induced difference spectra. Spectrochim Acta A 58: 1523–1533

    Article  Google Scholar 

  • Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution. Nature 411: 909–917

    Article  PubMed  CAS  Google Scholar 

  • Käss H, Fromme P, Witt HT and Lubitz W (2001) Orientation and electronic structure of the primary donor radical cation P − 700·+ in photosystem I: a single crystal EPR and ENDOR study. J Phys Chem B 105: 1225–1239

    Article  CAS  Google Scholar 

  • Kim S and Barry BA (2000) Identification of carbonyl modes of P700 by in situ chlorophyll labeling in photosystem I. J Am Chem Soc 122: 4980–4981

    Article  CAS  Google Scholar 

  • Kim S, Sacksteder CA, Bixby KA and Barry BA (2002) A reaction-induced FT-IR study of cyanobacterial photosystem I. Biochemistry 40: 15384–15395

    Article  CAS  Google Scholar 

  • Krabben L, Schlodder E, Jordan R, Carbonera D, Giacometti G, Lee H, Weber AN and Lubitz W (2000) Influence of the axial ligands on the spectral properties of P700 of photosystem I: a study of site-directed mutants. Biochemistry 39: 13012–13025

    Article  PubMed  CAS  Google Scholar 

  • Krauß N, Schubert W-D, Klukas OP, Fromme P, Witt HT and Saenger W (1996) Photosystem I at 4 Å resolution represents the first structural model of a joint photosynthetic reaction centre and core antenna system. Nature Struct Biol 3: 965–973

    Article  PubMed  Google Scholar 

  • Leibl W, Brettel K, Nabedryk E, Breton J, Rochaix J-D and Redding K (1998) Effect of PsaB-His656 → Leu mutation on optical and infrared difference spectra of P700 photooxidation. In: Garab G (ed) Photosynthesis: Mechanisms and Effects, Vol I, pp 595–598. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Li Y, Lucas M-G, Konovalova, T, Abbott B, MacMillan F, Petrenko A, Sivakumar V, Wang R, Hastings G, Gu F, van Tol J, Brunel L-C, Timkovich R, Rappaport F and Redding K (2004) Mutations of the putative hydrogen-bond donor to P700 of photosystem I. Biochemistry 43: 12634–12647

    Article  PubMed  CAS  Google Scholar 

  • Mäntele W (1993) Infrared vibrational spectroscopy of the photosynthetic reaction center. In: Deisenhofer H and Norris JR (eds) The Photosynthetic Reaction Center, Vol II, pp 239–283. Academic Press, San Diego

    Google Scholar 

  • Mäntele W, Wollenweber A, Rashwan F, Heinze J, Nabedryk E, Berger G and Breton J (1988) Fourier transform infrared spectroelectrochemistry of the bacteriochlorophyll a anion radical. Photochem Photobiol 47: 451–455

    Google Scholar 

  • Mezzetti A, Seo D, Leibl W, Sakurai H and Breton J (2003) Time-resolved step-scan FTIR investigation of the reaction center from the green sulfur bacterium Chlorobium tepidum. Photosynth Res 75: 161–169

    Article  PubMed  CAS  Google Scholar 

  • Nabedryk E (1996) Light-induced Fourier transform infrared difference spectroscopy of the primary electron donor in photosynthetic reaction centers. In: Mantsch HH and Chapman D (eds) Infrared Spectroscopy of Biomolecules, pp 39–81. Willey-Liss, New York

    Google Scholar 

  • Nabedryk E, Leonhard M, Mäntele W and Breton J (1990) Fourier transform infrared difference spectroscopy shows no evidence for an enolization of chlorophyll a upon cation formation either in vitro or during P700 photooxidation. Biochemistry 29: 3242–3247

    Article  PubMed  CAS  Google Scholar 

  • Nabedryk E, Leibl W and Breton J (1996) FTIR spectroscopy of primary donor photooxidation in photosystem I, Heliobacillus mobilis, and Chlorobium limicola. Comparison with purple bacteria. Photosynth Res 48: 301–308

    Article  CAS  Google Scholar 

  • Nabedryk E, Schulz C, Müh F, Lubitz W and Breton J (2000) Heterodimeric versus homodimeric structure of the primary electron donor in Rhodobacter sphaeroides reaction centers genetically modified at position M202. Photochem Photobiol 71: 582–588

    Article  PubMed  CAS  Google Scholar 

  • O’Malley PJ (2000) The effect of oxidation and reduction of chlorophyll a on its geometry, vibrational and spin density properties as revealed by hybrid density functional methods. J Am Chem Soc 122: 7798–7801

    Article  CAS  Google Scholar 

  • O’Malley PJ and Collins SJ (2001) The effect of axial Mg ligation on the geometry and spin density distribution of chlorophyll and bacteriochlorophyll cation free radical models: a density functional study. J Am Chem Soc 123: 11042–11046

    Article  PubMed  CAS  Google Scholar 

  • Pantelidou M, Chitnis P and Breton J (2004) FTIR spectroscopy of Synechocystis 6803 mutants affected on the hydrogen bonds to the carbonyl groups of the PsaA chlorophyll of P700 supports a model of extensive delocalization of the charge in P700+. Biochemistry 43: 8380–8390

    Article  PubMed  CAS  Google Scholar 

  • Parson WW, Chu Z-T and Warshel A (1990) Electrostatic control of charge separation in bacterial photosynthesis. Biochim Biophys Acta 1017: 251–272

    Article  PubMed  CAS  Google Scholar 

  • Plato M, Krauß N, Fromme P and Lubitz W (2003) Molecular orbital studies of the primary electron donor P700 of photosystem I based on a recent X-ray single crystal analysis. Chem Phys 294: 483–499

    Article  CAS  Google Scholar 

  • Redding K, MacMillan F, Leibl W, Brettel K, Hanley J, Rutherford AW, Breton J and Rochaix J-D (1998) A systematic survey of conserved histidines in the core subunits of photosystem I by site-directed mutagenesis reveals the likely axial ligands of P700. EMBO J 17: 50–60

    Article  PubMed  CAS  Google Scholar 

  • Sivakumar V, Wang R and Hastings G (2003) Photooxidation of P740, the primary electron donor in photosystem I from Acaryochloris marina. Biophys J 85: 3162–3172

    PubMed  CAS  Google Scholar 

  • Tavitian B, Nabedryk E, Mäntele W and Breton J (1986) Light-induced Fourier transform infrared (FTIR) spectroscopic investigations of primary reactions in photosystem I and photosystem II. FEBS Lett 201: 151–157

    Article  CAS  Google Scholar 

  • Wang R, Sivakumar V, Li Y, Redding K and Hastings G (2003) Mutation induced modulation of hydrogen bonding to P700 studied using FTIR difference spectroscopy. Biochemistry 42: 988–9897

    Google Scholar 

  • Wang R, Sivakumar V, Li Y, Johnson TW and Hastings G (2004) FTIR difference spectroscopy in combination with isotope labeling for identification of the carbonyl modes of P700 and P700+ in photosystem I. Biophys J 86: 1061–1073

    Article  PubMed  CAS  Google Scholar 

  • Weber AN and Lubitz W (2001) P700: the primary electron donor of photosystem I. Biochim Biophys Acta 1507: 61–79

    Article  Google Scholar 

  • Witt H, Schlodder E, Teutloff C, Niklas J, Bordignon E, Carbonera D, Kohler S, Labahn A and Lubitz W (2002) Hydrogen bonding to P700: site-directed mutagenesis of threonine A739 of photosystem I in Chlamydomonas reinhardtii. Biochemistry 41: 8557–8569

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de Bioénergétique, Bât. 532, CEA-Saclay, Gif-sur-Yvette, 91191, France

    Jacques Breton

Authors
  1. Jacques Breton
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Pennsylvania State University, Pennsylvania, USA

    John H. Golbeck

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Springer

About this chapter

Cite this chapter

Breton, J. (2006). FTIR Studies of the Primary Electron Donor, P700. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_18

Download citation

Publish with us

Policies and ethics

Search

Navigation