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Monitoring Gene Expression In Plant Tissues

Using green fluorescent protein with automated image collection and analysis

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Plant Tissue Culture Engineering

Part of the book series: Focus on Biotechnology ((FOBI,volume 6))

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References

  1. Kassler, M. (2001) Agricultural automation in the new millennium. Comput. Electron. Agric. 30: 237 240.

    Article  Google Scholar 

  2. Kacira, M. and Ling, P.P. (2001) Design and development of an automated and non-contact sensing system for continuous monitoring of plant health and growth. Trans. Am. Soc. Agric. Eng. 44: 989-996.

    Article  CAS  Google Scholar 

  3. Saiki, R.; Scharf, S.; Faloona, F.; Mullis, K.; Horn, G.; Erlich, H. and Arnheim, N. (1985) A novel method for the parental diagnosis of sickle cell anemia. Am. J. Hum. Gene. 37: A172.

    Google Scholar 

  4. Lee, M.S.; Chang, K.S.; Cabanillas, F.; Freireich, E.J.; Trujillo, J.M. and Stass, S.M. (1987) Detection of minimal residual cells carrying the T-14 18 by DNA sequence amplification. Science 237: 175-178.

    Article  CAS  PubMed  Google Scholar 

  5. Schena, M.; Shalon, D.; Heller, R.; Chai, A.; Brown, P.O. and Davis, R.W. (1996) Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. - USA 93: 10614-10619.

    Article  CAS  Google Scholar 

  6. Kazan, K.; Schenk, P.M.; Wilson, I. and Manners, J.M. (2001) DNA microarrays: New tools in the analysis of plant defense responses. Mol. Plant Path. 2: 177-185.

    Article  CAS  Google Scholar 

  7. Khan, J.; Wei, J.S.; Ringner, M.; Saal, L.H.; Ladanyi, M.; Westermann, F.; Berthold, F.; Schwab, M.; Antonescu, C.R.; Peterson, C. and Meltzer, P.S. (2001) Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks. Nature Medicine 7: 673-679.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Arcellana-Panlilio, M. and Robbins, S.M. (2002) Cutting-edge technology I. Global gene expression profiling using DNA microarrays. Am. J. Physiol. 282: G397-G402.

    CAS  Google Scholar 

  9. Eisen, M.B. and Brown, P.O. (1999) cDNA Preparation and Characterization. In: Weissman, S. (Ed.) DNA arrays for analysis of gene expression. Academic Press, New York; pp.179-205.

    Google Scholar 

  10. Finer, J.J.; Finer, K.R. and Santarem, E.R. (1996) Plant cell transformation, physical methods for. In: Meyers, R.A. (Ed.) Encyclopedia of Molecular Biology and Molecular Medicine. VCH Publishers, The Netherlands; pp. 458-465.

    Google Scholar 

  11. Klein, T.M.; Wolf, E.D.; Wu, R. and Sanford, J.C. (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70-73.

    Article  CAS  Google Scholar 

  12. Horsch, R.B.; Fry, J.E.; Hoffman, N.L.; Eicholtz, D.; Rogers, S.G. and Fraley, R.T. (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231.

    Article  CAS  Google Scholar 

  13. Finer, J.J.; Vain, P.; Jones, M.W. and McMullen, M.D.(1992) Development of the Particle Inflow Gun for DNA delivery to plant cells. Plant Cell Rep. 11: 232-238.

    Article  Google Scholar 

  14. Sanford, J.C.; DeVit, M.J.; Russell, J.A.; Smith, F.D.; Harpending, P.R.; Roy, M.K. and Johnston, S.A. (1991) An improved, helium-driven biolistic device. Technique 3: 3-16.

    CAS  Google Scholar 

  15. Vain, P.; McMullen, M.D. and Finer, J.J. (1993) Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize. Plant Cell Rep. 12: 84-88.

    Article  CAS  PubMed  Google Scholar 

  16. Hadi, M.Z.; McMullen, M.D. and Finer, J.J. (1996) Transformation of 12 different plasmids into soybean via particle bombardment. Plant Cell Rep. 15: 500 -505.

    Article  CAS  PubMed  Google Scholar 

  17. Kohli, A.; Griffiths, S.; Palacios, N.; Twyman, R.M.; Vain, P.; Laurie, D. and Christou, P. (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination. Plant J. 17: 591-601.

    Article  CAS  PubMed  Google Scholar 

  18. Fu, X.; Duc, L.T.; Fontana, S.; Bong, B.B.; Tinjuangjun, P,; Sudhakar, D.; Twyman, R.M.; Christou, P. and Kohli, A. (2000) Linear transgene constructs lacking vector backbone sequences generate low-copynumber transgenic plants with simple integration patterns. Trans. Res. 9: 11-19.

    Article  CAS  Google Scholar 

  19. Napoli, C.; Lemieux, C. and Jorgensen, R. (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-supression of homologous genes in trans. Plant Cell 2: 279-289.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Chilton, M.D.; Drummond, M.J.; Merlo, D.J.; Sciaky, D.; Montoya, A.L.; Gordon, M.P. and Nester, E.W. (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11: 263-271.

    Article  CAS  PubMed  Google Scholar 

  21. Hansen, G.; Das, A. and Chilton, M.D. (1994) Constitutive expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium. Proc. Natl. Acad. Sci.- USA 91: 7603-7607.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Stachel, S.E.; Messens, E.; Van Montagu, M. and Zambryski, P. (1985) Identification of the signal molecules produced by wounded plant cells which activate the T-DNA transfer process in Agrobacterium tumefaciens. Nature 318: 624-629.

    Article  Google Scholar 

  23. Trick, H.N. and Finer, J.J. (1997) SAAT: Sonication Assisted Agrobacterium-mediated Transformation. Transgenic Res. 6: 329-336.

    Article  CAS  Google Scholar 

  24. Zupan, J.; Muth, T.R.; Draper, O. and Zambryski, P. (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J. 23: 11-28.

    Article  CAS  PubMed  Google Scholar 

  25. Ponappa, T.; Brzozowski, A.E. and Finer, J.J. (1999) Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein (GFP). Plant Cell Rep. 19: 6-12.

    Article  CAS  PubMed  Google Scholar 

  26. Hunold, R.; Bronner, R. and Hahne, G. (1994) Early events in microprojectile bombardment: cell viability and particle location. Plant J. 5: 593-604.

    Article  CAS  Google Scholar 

  27. Svitashev, S.K.; Pawlowski, W.P.; Makarevitch, I.; Plank, D.W. and Somers, D.A. (2002) Complex transgene locus structures implicate multiple mechanisms for plant transgene rearrangement. Plant J. 32: 433–445.

    Article  CAS  PubMed  Google Scholar 

  28. Jefferson, R.A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387-405.

    Article  CAS  Google Scholar 

  29. Ridgway, E.B. and Ashley, C.C. (1967) Calcium transients in single muscle fibers. Biochem. Biophys. Res. Commun. 29: 229–230.

    Article  CAS  PubMed  Google Scholar 

  30. Niedz, R.P.; Sussman, M.R. and Satterlee, J.S. (1995) Green Fluorescent protein: an in vivo reporter of plant gene expression. Plant Cell Rep. 14: 403-406.

    Article  CAS  PubMed  Google Scholar 

  31. Stewart, C.N. (2001) The utility of green fluorescent protein in transgenic plants. Plant Cell Rep. 20: 376-382.

    Article  CAS  PubMed  Google Scholar 

  32. Haseloff, J. and Amos, B. (1995) GFP in plants. Trends in Genetics 8: 328-329.

    Article  Google Scholar 

  33. Vanden Wymelenberg, A.J.; Cullen, D.; Spear, R.N.; Schoenike, B. and Andrews, J.H. (1997) Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. BioTechniques 23: 686-690.

    Article  CAS  PubMed  Google Scholar 

  34. Haseloff, J. and Siemering, K.R. (1998) The uses of green fluorescent protein in plants. In: Chalfie, M. (Ed.) Green Fluorescent Protein: Properties, Application, and Protocols. Wiley-Liss, Inc., New York; pp. 191-219.

    Google Scholar 

  35. Nagatani, N.; Takuni, S.; Tomiyama, M.; Shimada, T. and Tamiya, E. (1997) Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice. Plant Sci.124: 49-56.

    Article  CAS  Google Scholar 

  36. Grebenok, R.J.; Lambert, G.M. and Galbraith, D.W. (1997) Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants. Plant J. 12: 685-696.

    Article  CAS  Google Scholar 

  37. Piston, D.W.; Patterson, G.H. and Knobel, S.M. (1999) Quantitative imaging of the green fluorescent protein (GFP). In: Methods in Cell Biology, Nashville, Tennessee; pp. 31-48.

    Google Scholar 

  38. Maximova, S.N.; Dandekar, A.M.; and Guiltinan, M.J. (1998) Investigation of Agrobacterium-mediated transformation of apple using green fluorescent protein: high transient expression and low stable transformation suggest that factors other than T-DNA transfer are rate-limiting. Plant Molec. Biol. 37: 549 – 559.

    Article  CAS  Google Scholar 

  39. Hauser, K.; Haynes, W.J.; Kung, C.; Plattner, H. and Kissmehl, R. (2000) Expression of the green fluorescent protein in Paramecium tetraurelia. Eur. J. Cell Biol. 79: 144-149.

    Article  CAS  PubMed  Google Scholar 

  40. Spear, R.N.; Cullen, D. and Andrews, J.H. (1999) Fluorescent labels, confocal microscopy, and quantitative image analysis in study of fungal biology. In:Methods in Enzymology, Vol. 307: 607-623.

    Article  CAS  Google Scholar 

  41. Scholz, O.; Thiel, A.; Hillen, W. and Niederweis, M. (2000) Quantitative analysis of gene expression with an improved green fluorescent protein. Eur. J. Biochem. 267: 1565-1570.

    Article  CAS  PubMed  Google Scholar 

  42. Inoué, S.; Shimomura, O.; Goda, M.; Shribak, M. and Tran, P.T. (2002) Fluorescence polarization of green fluorescence protein. Proc. Natl. Acad. Sci. -USA 99: 4272-4277.

    Article  CAS  Google Scholar 

  43. Buenrostro-Nava, M.T.; Ling, P.P. and Finer, J.J. (2003) Development of an automated image collection system for generating time-lapse animations of plant tissue growth and green fluorescent protein gene expression. In: Vasil, I.K. (Ed.) Plant Biotechnology 2002 and Beyond. Kluwer Academic Publishers, The Netherlands; pp. 293-295.

    Chapter  Google Scholar 

  44. Buenrostro-Nava, M.T.; Ling, P.P. and Finer, J.J. (2005) Development of an automated image acquisition system for monitoring gene expression and tissue growth. Trans. Am. Soc. Agric. Eng. (in press).

    Google Scholar 

  45. Svedlow, M.; McGillem, C.D. and Anuta, P.E. (1978)Image registration: similarity measure and preprocessing method comparisons. Aerospace and Electronic Systems, IEEE Trans. AES 14: 141-149.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Horticulture and Crop Science, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA

    John J. Finer, Summer L. Beck & Marco T. Buenrostro-Nava

  2. Department of Food, Agricultural and Biological Engineering,OARDC/The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA

    YU-TSEH CHI & PETER P. LING

  3. DuPont Agriculture and Nutrition, Rt. 141 and Henry Clay Road, Wilmington, DE 19880, USA

    Summer L. Beck

  4. IREGEP, Colegio de PostgraduadosIREGEP, Colegio de Postgraduados, Carretera Mexico-Texcoco Km 35.5 Montecillo, Texcoco, Mexico

    Marco T. Buenrostro-Nava

  5. 57 228 Lane Section, 3 Yuanji Rd., Tianjhong Town, Chang-Hua 520, Taiwan

    YU-TSEH CHI

Authors
  1. John J. Finer
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  2. Summer L. Beck
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  3. Marco T. Buenrostro-Nava
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  4. YU-TSEH CHI
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  5. PETER P. LING
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Editor information

Editors and Affiliations

  1. Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, India

    S. Dutta Gupta

  2. Department of Biological Science, Yamaguchi University, Yamaguchi, Japan

    Yasuomi Ibaraki

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© 2008 Springer

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Finer, J.J., Beck, S.L., Buenrostro-Nava, M.T., CHI, YT., LING, P.P. (2008). Monitoring Gene Expression In Plant Tissues. In: Gupta, S.D., Ibaraki, Y. (eds) Plant Tissue Culture Engineering. Focus on Biotechnology, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-3694-1_2

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