Some Aspects of Recovery of [14C]-Allantoin in the Urine of Sheep
Two Experiments were carried out to determine the extent of variation in the recovery of [14C]-allantoin in the urine of sheep given a general purpose diet at three levels of intake. In Experiment I, the level of feed intake did not affect the recovery of plasma [14C]-allantoin in the urine of sheep but there was a significant difference between individuals on the recovery (P <0.05). In contrast, in Experiment II there was no effect of animals on the recovery when four sheep were given the same level of feed intake. However, the effect of period on [14C]-allantoin recovered in the urine was statistically significant (P <0.05). The proportion of plasma [14C]-allantoin excreted in urine of eight sheep injected intravenously with labelled allantoin in Experiment I and II varied from 66–95% (mean 83 ±SE 1.6%) showing the variation between individuals within species.
KeywordsCatheter HPLC Filtration Syringe Purine
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