Skip to main content

Genetic and Transgenic Approaches to Improving Crop Performance

  • Chapter
Plant Hormones

Abstract

As this volume illustrates, plant hormones play important roles in all aspects of the growth and development of plants, and in their interactions with the environment and other organisms. Many of the developmental and physiological processes regulated by plant hormones are of agronomic importance: examples that will be further explored in this chapter include the roles of gibberellins in regulating stem elongation and of ethylene in promoting fruit ripening. As a consequence of the central roles of plant hormones, plant hormone signalling has been a target of genetic modification by various selective processes, from the evolution of land plants 400 million years ago, through the adaptation of species to specific ecological niches, the domestication of crop plants by prehistoric farmers, the directed breeding of improved varieties with the advent of modern genetics 100 years ago and, most recently, the targeted modification of hormone signaling pathways in transgenic plants to improve specific agronomic traits.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ashikari, M., Sasaki, A., Ueguchi-Tanaka, M., Itoh, H., Nishimura, A., Datta, S., Ishiyama, K., Saito, T., Kobayashi, M., Khush, G.S., Kitano, H. and Matsuoka, M. (2002) Loss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox- 2), led to the rice 'green revolution'. Breed. Sci., 52, 143-150.

    Article  CAS  Google Scholar 

  2. Baldwin, I.T. (1998) Jasmonate-induced responses are costly but benefit plants under attack in native populations. Proc. Natl. Acad. Sci. U.S.A., 95, 8113-8118.

    Article  CAS  PubMed  Google Scholar 

  3. Bovy, A.G., Angenent, G.C., Dons, H.J.M. and van Altvorst, A.C. (1999) Heterologous expression of the Arabidopsis etr1-1 allele inhibits the senescence of carnation flowers. Mol. Breed., 5, 301-308.

    Article  CAS  Google Scholar 

  4. Carrera, E., Bou, J., Garcia-Martinez, J.L. and Prat, S. (2000) Changes in GA 20- oxidase gene expression strongly affect stem length, tuber induction and tuber yield of potato plants. Plant J., 22, 247-256.

    Article  CAS  PubMed  Google Scholar 

  5. Chiang, H.H., Hwang, I. and Goodman, H.M. (1995) Isolation of the Arabidopsis GA4 locus. Plant Cell, 7, 195-201.

    Article  CAS  PubMed  Google Scholar 

  6. Coles, J.P., Phillips, A.L., Croker, S.J., Garcia-Lepe, R., Lewis, M.J. and Hedden, P. (1999) Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20-oxidase genes. Plant J., 17, 547-556.

    Article  CAS  PubMed  Google Scholar 

  7. Curtis, I.S., Ward, D.A., Thomas, S.G., Phillips, A.L., Davey, M.R., Power, J.B., Lowe, K.C., Croker, S.J., Lewis, M.J., Magness, S.L. and Hedden, P. (2000) Induction of dwarfism in transgenic Solanum dulcamara by over-expression of a gibberellin 20- oxidase cDNA from pumpkin. Plant J., 23, 329-338.

    Article  CAS  PubMed  Google Scholar 

  8. Diaz, J., ten Have, A. and van Kan, J.A.L. (2002) The role of ethylene and wound signaling in resistance of tomato to Botrytis cinerea. Plant Physiol., 129, 1341-1351.

    Article  CAS  PubMed  Google Scholar 

  9. Donzella, G., Spena, A. and Rotino, G.L. (2000) Transgenic parthenocarpic eggplants: superior germplasm for increased winter production. Mol. Breed., 6, 79-86.

    Article  Google Scholar 

  10. Eriksson, M.E., Israelsson, M., Olsson, O. and Moritz, T. (2000) Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length. Nature Biotechnol., 18, 784-788.

    Article  CAS  Google Scholar 

  11. Evans, L.T. (1998) Feeding the Ten Billion: Plant and Population Growth. Cambridge University Press, Cambridge.

    Google Scholar 

  12. Ficcadenti, N., Sestili, S., Pandolfini, T., Cirillo, C., Rotino, G.L. and Spena, A. (1999) Genetic engineering of parthenocarpic fruit development in tomato. Mol. Breed., 5, 463-470.

    Article  Google Scholar 

  13. Fleet, C.M., Yamaguchi, S., Hanada, A., Kawaide, H., David, C.J., Kamiya, Y. and Sun, T.-p. (2003) Overexpression of AtCPS and AtKS in Arabidopsis confers increased entkaurene production but no increase in bioactive gibberellins. Plant Physiol., 132, 830-839.

    Article  CAS  PubMed  Google Scholar 

  14. Flintham, J.E., Borner, A., Worland, A.J. and Gale, M.D. (1997) Optimizing wheat grain yield: Effects of Rht (gibberellin-insensitive) dwarfing genes. J. Agric. Sci., 128, 11-25.

    Article  Google Scholar 

  15. Flores, F.B., Martinez-Madrid, M.C., Sanchez-Hidalgo, F.J. and Romojaro, F. (2001) Differential rind and pulp ripening of transgenic antisense ACC oxidase melon. Plant Physiol. Biochem., 39, 37-43.

    Article  Google Scholar 

  16. Fos, M., Proano, K., Nuez, F. and Garcia-Martinez, J.L. (2001) Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato. Physiol. Plant., 111, 545-550.

    Article  CAS  PubMed  Google Scholar 

  17. Fu, X.D., Sudhakar, D., Peng, J.R., Richards, D.E., Christou, P. and Harberd, N.P. (2001) Expression of arabidopsis GAI in transgenic rice represses multiple gibberellin responses. Plant Cell, 13, 1791-1802.

    Article  CAS  PubMed  Google Scholar 

  18. Gan, S.S. and Amasino, R.M. (1995) Inhibition of leaf senescence by autoregulated production of cytokinin. Science, 270, 1986-1988.

    Article  CAS  PubMed  Google Scholar 

  19. Gianfagna, T. (1995) Natural and synthetic growth regulators and their use in horticultural and agronomic crops. In: Plant Hormones. Davies, P. J. (ed.). Dordrecht: Kluwer, pp. 751-773.

    Google Scholar 

  20. Good, X., Kellogg, J.A., Wagoner, W., Langhoff, D., Matsumura, W. and Bestwick, R.K. (1994) Reduced ethylene synthesis by transgenic tomatoes expressing Sadenosylmethionine hydrolase. Plant Mol. Biol., 26, 781-790.

    Article  CAS  PubMed  Google Scholar 

  21. Harms, K., Atzorn, R., Brash, A., Kühn, H., Wasternack, C., Willmitzer, L. and Peña- Cortés, H. (1995) Expression of a flax allene oxide synthase cDNA leads to increased endogenous jasmonic acid (JA) levels in transgenic potato plants but not to a corresponding activation of JA-responding genes. Plant Cell, 7, 1645-1654.

    Article  CAS  PubMed  Google Scholar 

  22. Hedden, P. and Phillips, A.L. (2000) Gibberellin metabolism: new insights revealed by the genes. Trends Plant Sci., 5, 523-530.

    Article  CAS  PubMed  Google Scholar 

  23. Hedden, P. and Phillips, A.L. (2000) Manipulation of hormone biosynthetic genes in transgenic plants. Curr. Opin. Biotechnol., 11, 130-137.

    Article  CAS  PubMed  Google Scholar 

  24. Henzi, M.X., Christey, M.C. and McNeil, D.L. (2000) Morphological characterisation and agronomic evaluation of transgenic broccoli (Brassica oleracea L. var. italica) containing an antisense ACC oxidase gene. Euphytica, 113, 9-18.

    Article  CAS  Google Scholar 

  25. Hrazdina, G., Kiss, E., Galli, Z., Rosenfield, C., Norelli, J.L. and Aldwinckle, H.S. (2003) Down Regulation of Ethylene Production in 'Royal Gala' Apples. Acta Horticulturae, 628, in press.

    Google Scholar 

  26. Huang, S.S., Raman, A.S., Ream, J.E., Fujiwara, H., Cerny, R.E. and Brown, S.M. (1998) Overexpression of 20-oxidase confers a gibberellin- overproduction phenotype in Arabidopsis. Plant Physiol., 118, 773-781.

    Article  CAS  PubMed  Google Scholar 

  27. Itoh, H., Ueguchi-Tanaka, M., Sakamoto, T., Kayano, T., Tanaka, H., Ashikari, M. and Matsuoka, M. (2002) Modification of rice plant height by suppressing the heightcontrolling gene, D18, in rice. Breed. Sci., 52, 215-218.

    Article  CAS  Google Scholar 

  28. John, I., Drake, R., Farrell, A., Cooper, W., Lee, P., Horton, P. and Grierson, D. (1995) Delayed leaf senescence in ethylene-deficient ACC oxidase antisense tomato plants - molecular and physiological analysis. Plant J., 7, 483-490.

    Article  CAS  Google Scholar 

  29. Jones, H.D., Peters, N.C.B. and Holdsworth, M.J. (1997) Genotype and environment interact to central dormancy and differential expression of the VIVIPAROUS-1 homologue in embryos of Avena fatua. Plant J., 12, 911-920.

    Article  CAS  PubMed  Google Scholar 

  30. Klee, H.J. (1993) Ripening physiology of fruit from transgenic tomato (Lycopersicon esculentum) plants with reduced ethylene synthesis. Plant Physiol., 102, 911-916.

    CAS  PubMed  Google Scholar 

  31. Klee, H.J., Hayford, M.B., Kretzmer, K.A., Barry, G.F. and Kishore, G.M. (1991) Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell, 3, 1187-1193.

    Article  CAS  PubMed  Google Scholar 

  32. Koornneef, M., Elgersma, A., Hanhart, C.J., van Loenen-Martinet, E.P., van Rijn, L. and Zeevaart, J.A.D. (1985) A gibberellin insensitive mutant of Arabidopsis thaliana. Physiol. Plant., 65, 33-39.

    Article  CAS  Google Scholar 

  33. Kosugi, Y., Waki, K., Iwazaki, Y., Tsuruno, N., Mochizuki, A., Yoshioka, T., Hashiba, T. and Satoh, S. (2002) Senescence and gene expression of transgenic non-ethyleneproducing carnation flowers. J. Jpn. Soc. Hortic. Sci., 71, 638-642.

    Article  CAS  Google Scholar 

  34. Laudert, D., Schaller, F. and Weiler, E.W. (2000) Transgenic Nicotiana tabacum and Arabidopsis thaliana plants overexpressing allene oxide synthase. Planta, 211, 163-165.

    Article  CAS  PubMed  Google Scholar 

  35. Lester, D.R., Ross, J.J., Davies, P.J. and Reid, J.B. (1997) Mendel's stem length gene (Le) encodes a gibberellin 3 beta- hydroxylase. Plant Cell, 9, 1435-1443.

    Article  CAS  PubMed  Google Scholar 

  36. Lund, S.T., Stall, R.E. and Klee, H.J. (1998) Ethylene regulates the susceptible response to pathogen infection in tomato. Plant Cell, 10, 371-382.

    Article  CAS  PubMed  Google Scholar 

  37. Martin, D.N., Proebsting, W.M. and Hedden, P. (1997) Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins. Proc. Natl. Acad. Sci. U.S.A., 94, 8907-8911.

    Article  CAS  PubMed  Google Scholar 

  38. Martin, D.N., Proebsting, W.M. and Hedden, P. (1999) The SLENDER gene of pea encodes a gibberellin 2-oxidase. Plant Physiol., 121, 775-781.

    Article  CAS  PubMed  Google Scholar 

  39. Martinez-Garcia, J.F., Santes, C.M. and Garcia-Martinez, J.L. (2000) The end-of-day far-red irradiation increases gibberellin A1 content in cowpea (Vigna sinensis) epicotyls by reducing its inactivation. Physiol. Plant., 108, 426-434.

    CAS  Google Scholar 

  40. McCallum, C.M., Comai, L., Greene, E.A. and Henikoff, S. (2000) Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol., 123, 439-442.

    Article  CAS  PubMed  Google Scholar 

  41. McKibbin, R.S., Wilkinson, M.D., Bailey, P.C., Flintham, J.E., Andrew, L.M., Lazzeri, P.A., Gale, M.D., Lenton, J.R. and Holdsworth, M.J. (2002) Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proc. Natl. Acad. Sci. U.S.A., 99, 10203-10208.

    Article  CAS  PubMed  Google Scholar 

  42. Monna, L., Kitazawa, N., Yoshino, R., Suzuki, J., Masuda, H., Maehara, Y., Tanji, M., Sato, M., Nasu, S. and Minobe, Y. (2002) Positional cloning of rice semidwarfing gene, sd-1: Rice "Green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. DNA Res., 9, 11-17.

    Article  CAS  PubMed  Google Scholar 

  43. Peng, J.R., Carol, P., Richards, D.E., King, K.E., Cowling, R.J., Murphy, G.P. and Harberd, N.P. (1997) The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes Dev., 11, 3194-3205.

    Article  CAS  PubMed  Google Scholar 

  44. Peng, J.R., Richards, D.E., Hartley, N.M., Murphy, G.P., Devos, K.M., Flintham, J.E., Beales, J., Fish, L.J., Worland, A.J., Pelica, F., Sudhakar, D., Christou, P., Snape, J.W., Gale, M.D. and Harberd, N.P. (1999) 'Green revolution' genes encode mutant gibberellin response modulators. Nature, 400, 256-261.

    Article  CAS  PubMed  Google Scholar 

  45. Petty, L.M., Harberd, N.P., Carre, I.A., Thomas, B. and Jackson, S.D. (2003) Expression of the Arabidopsis gai gene under its own promoter causes a reduction in plant height in chrysanthemum by attenuation of the gibberellin response. Plant Sci., 164, 175-182.

    Article  CAS  Google Scholar 

  46. Phillips, A.L., Ward, D.A., Uknes, S., Appleford, N.E.J., Lange, T., Huttly, A.K., Gaskin, P., Graebe, J.E. and Hedden, P. (1995) Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis. Plant Physiol., 108, 1049-1057.

    Article  CAS  PubMed  Google Scholar 

  47. Picton, S., Barton, S.L., Bouzayen, M., Hamilton, A.J. and Grierson, D. (1993) Altered fruit ripening and leaf senescence in tomatoes expressing an antisense ethylene-forming enzyme transgene. Plant J., 3, 469-481.

    CAS  Google Scholar 

  48. Qin, X.Q. and Zeevaart, J.A.D. (2002) Overexpression of a 9-cis-epoxycarotenoid dioxygenase gene in Nicotiana plumbaginifolia increases abscisic acid and phaseic acid levels and enhances drought tolerance. Plant Physiol., 128, 544-551.

    Article  CAS  PubMed  Google Scholar 

  49. Rademacher, W. (2000) Growth retardants: Effects on gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol., 51, 501-531.

    Article  CAS  Google Scholar 

  50. Rotino, G.L., Perri, E., Zottini, M., Sommer, H. and Spena, A. (1997) Genetic engineering of parthenocarpic plants. Nature Biotechnol., 15, 1398-1401.

    Article  CAS  Google Scholar 

  51. Sakamoto, T., Kamiya, N., Ueguchi-Tanaka, M., Iwahori, S. and Matsuoka, M. (2001) KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem. Genes Dev., 15, 581-590.

    Article  CAS  PubMed  Google Scholar 

  52. Sakamoto, T., Kobayashi, M., Itoh, H., Tagiri, A., Kayano, T., Tanaka, H., Iwahori, S. and Matsuoka, M. (2001) Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice. Plant Physiol., 125, 1508-1516.

    Article  CAS  PubMed  Google Scholar 

  53. Sakamoto, T., Morinaka, Y., Ishiyama, K., Kobayashi, M., Itoh, H., Kayano, T., Iwahori, S., Matsuoka, M. and Tanaka, H. (2003) Genetic manipulation of gibberellin metabolism in transgenic rice. Nature Biotechnol., 21, 909-913.

    Article  CAS  Google Scholar 

  54. Spielmeyer, W., Ellis, M.H. and Chandler, P.M. (2002) Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. Proc. Natl. Acad. Sci. U.S.A., 99, 9043-9048.

    Article  CAS  PubMed  Google Scholar 

  55. Tamminen, I., Makela, P., Heino, P. and Palva, E.T. (2001) Ectopic expression of ABI3 gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis thaliana. Plant J., 25, 1-8.

    Article  CAS  PubMed  Google Scholar 

  56. Tan, B.C., Schwartz, S.H., Zeevaart, J.A.D. and McCarty, D.R. (1997) Genetic control of abscisic acid biosynthesis in maize. Proc. Natl. Acad. Sci. U.S.A., 94, 12235-12240.

    Article  CAS  PubMed  Google Scholar 

  57. Thomas, S.G., Phillips, A.L. and Hedden, P. (1999) Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation. Proc. Natl. Acad. Sci. U.S.A., 96, 4698-4703.

    Article  CAS  PubMed  Google Scholar 

  58. Thompson, A.J., Jackson, A.C., Symonds, R.C., Mulholland, B.J., Dadswell, A.R., Blake, P.S., Burbidge, A. and Taylor, I.B. (2000) Ectopic expression of a tomato 9-cisepoxycarotenoid dioxygenase gene causes over-production of abscisic acid. Plant J., 23, 363-374.

    Article  CAS  PubMed  Google Scholar 

  59. Verberne, M.C., Verpoorte, R., Bol, J.F., Mercado-Blanco, J. and Linthorst, H.J.M. (2000) Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance. Nature Biotechnol., 18, 779-783.

    Article  CAS  Google Scholar 

  60. Vidal, A.M., Gisbert, C., Talon, M., Primo-Millo, E., Lopez-Diaz, I. and Garcia- Martinez, J.L. (2001) The ectopic overexpression of a citrus gibberellin 20-oxidase enhances the non-13-hydroxylation pathway of gibberellin biosynthesis and induces an extremely elongated phenotype in tobacco. Physiol. Plant., 112, 251-260.

    Article  CAS  PubMed  Google Scholar 

  61. Wang, C., Avdiushko, S. and Hildebrand, D.F. (1999) Overexpression of a cytoplasmlocalized allene oxide synthase promotes the wound-induced accumulation of jasmonic acid in transgenic tobacco. Plant Mol. Biol., 40, 783-793.

    Article  CAS  PubMed  Google Scholar 

  62. White, C.N., Proebsting, W.M., Hedden, P. and Rivin, C.J. (2000) Gibberellins and seed development in maize. I. Evidence that gibberellin/abscisic acid balance governs germination versus maturation pathways. Plant Physiol., 122, 1081-1088.

    Article  CAS  PubMed  Google Scholar 

  63. Wilkinson, J.Q., Lanahan, M.B., Clark, D.G., Bleecker, A.B., Chang, C., Meyerowitz, E.M. and Klee, H.J. (1997) A dominant mutant receptor from Arabidopsis confers ethylene insensitivity in heterologous plants. Nature Biotechnol., 15, 444-447.

    Article  CAS  Google Scholar 

  64. Wilkinson, J.Q., Lanahan, M.B., Yen, H.-C., Giovannoni, J.J. and Klee, H.J. (1995) An ethylene-inducible component of signal transduction encoded by Never-ripe. Science, 270, 1807-1809.

    Article  CAS  PubMed  Google Scholar 

  65. Xu, Y.L., Gage, D.A. and Zeevaart, J.A.D. (1997) Gibberellins and stem growth in Arabidopsis thaliana - effects of photoperiod on expression of the GA4 and GA5 loci. Plant Physiol., 114, 1471-1476.

    Article  CAS  PubMed  Google Scholar 

  66. Xu, Y.L., Li, L., Gage, D.A. and Zeevaart, J.A.D. (1999) Feedback regulation of GA5 expression and metabolic engineering of gibberellin levels in Arabidopsis. Plant Cell, 11, 927-935.

    Article  CAS  PubMed  Google Scholar 

  67. Yamaguchi, S., Smith, M.W., Brown, R.G.S., Kamiya, Y. and Sun, T.P. (1998) Phytochrome regulation and differential expression of gibberellin 3β-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell, 10, 2115-2126.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Andy L. Phillips .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer Science+Business Media B.V.

About this chapter

Cite this chapter

Phillips, A.L. (2010). Genetic and Transgenic Approaches to Improving Crop Performance. In: Davies, P.J. (eds) Plant Hormones. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2686-7_27

Download citation

Publish with us

Policies and ethics