Approaches to Molecular Genetics and Genomics of Botrytis
Molecular genetic techniques have revolutionized the detailed analysis of infection strategies and pathogenicity of Botrytis. Based on the availability of all necessary molecular tools, an impressive (and rapidly growing) number of genes has been functionally analysed by targeted inactivation approaches. The result of these studies, taken together with the new opportunities arising from "genomics" of B. cinerea, has developed into one of the model systems for molecular phytopathology. The methodologies and tools available for the cloning of genes and their functional analysis will be discussed and a compilation given of the deletion mutants obtained so far (dealt with in detail in other chapters). Current trends and perspectives of this rapidly developing field are discussed.
KeywordsGlutathione Lipase Enzymatic Degradation NADPH Catalase
Unable to display preview. Download preview PDF.
- Arranz M, Eslava AP, Diaz-Minguez IM and Benito EP (2003) Hypervirulent strains of Botrytis cinerea show altered respiration. XXII Fungal Genetics Conference, Asilomar (USA) abstract p 385Google Scholar
- Banuett F (2002) Pathogenic development in Ustilago maydis. In: Osiewacz H (ed.) Molecular Biology of Fungal Development. (pp. 349-398) Marcel Dekker, New York, USAGoogle Scholar
- Hayashi K, Schoonbeek HJ and De Waard MA (2002b) Bcmfs1, a novel major facilitator superfamily transporter from Botrytis cinerea, provides tolerance towards the natural toxic compounds camptothecin and cercosporin and towards fungicides. Applied and Environmental Microbiology 68: 4996-5004CrossRefGoogle Scholar
- Kunz C, Poinssot B, Pugin A and Boccara M (2002) Characterization of a non-pathogenic mutant of Botrytis cinerea. 6th European Conference on Fungal Genetics, Pisa, p. 267Google Scholar
- Oeser B, Tenberge KB, Moore S, Mihlan M, Heidrich PM and Tudzynski P (2002) Pathogenic development of Claviceps purpurea. In: Osiewacz H (ed.) Molecular Biology of Fungal Development. (pp. 419-455) Marcel Dekker, New York, USAGoogle Scholar
- Rolke Y, Liu S, Quidde T, Williamson B, Schouten A, Weltring, K-M, Siewers V, Tenberge KB, Tudzynski B and Tudzynski P (2004) Functional analysis of H2O2-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable. Molecular Plant Pathology 5: 17-27CrossRefGoogle Scholar
- Rolland S, Jobic C, Fèvre M and Bruel C (2003) Agrobacterium-mediated transformation of Botrytis cinerea, simple purification of monokaryotic transformants and rapid conidia-based identification of the transfer-DNA host genomic DNA flanking sequences. Current Genetics 44: 164-171CrossRefPubMedGoogle Scholar
- Tudzynski B and Tudzynski P (2002) Pathogenicity factors and signal transduction in plant-pathogenic fungi. Progress in Botany 63: 163-188Google Scholar
- Verhoeff K, Malathrakis NE and Williamson B (1992) Recent Advances in Botrytis Research. Pudoc Scientific Publishers, Wageningen, The NetherlandsGoogle Scholar
- Viaud M, Brunet-Simon A, Brygoo Y, Pradier J-M and Levis C (2003) Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea. Molecular Microbiology 50: 1451-1465CrossRefPubMedGoogle Scholar