Regulation of Electron Transport in the Respiratory Chain of Plant Mitochondria

  • Francis E. Sluse
  • Wieslawa Jarmuszkiewicz
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 17)


The aim of this chapter is to gather updated understanding of electron flow and H+ flux-linked processes in plant mitochondria. New understanding of molecular mechanisms of redox pumps and ATP synthase are described. Special attention is paid to the non-phosphorylating electron flow pathways and to H+ electrochemical gradient consumers. Partitioning of electrons between ubiquinol oxidizing pathways (alternative oxidase, AOX, and cytochrome pathway) is analyzed at the level of methodological aspects and interpreted in terms of energy conservation efficiency and of cell metabolism regulation. Partitioning of H+ electrochemical gradient between ATP synthase and uncoupling protein (UCP) is tackled through the ADP/O method. It is shown how efficiently UCP activity can divert energy from oxidative phosphorylation. It is also stressed that AOX and UCP are not redundant as cnergy-dissipating proteins because AOX is inhibited by free fatty acids that stimulate UCP and because AOX and UCP have completely different kinetic behavior regarding the redox State of ubiquinone. It is concluded that we are still very far from having a clear idea about the physiological role and interplay of energy-wasting and energy-conserving Systems.


Linoleic Acid Tomato Fruit Uncouple Protein Alternative Oxidase Plant Mitochondrion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



alternative oxidase


benzohydroxamic acid

complex I

NADH: ubiquinone oxidoreductase

complex II

succinate: ubiquinone oxidoreductase

complex III

ubiquinol: ubiquinone cytochrome c oxidoreductase

complex IV

cytochrome c oxidoreductase


H+ electrochemical gradient


pH difference between the intermembrane and matrix spaces


mitochondrial transmembrane electrical potential, Ψin—Ψout


electron transfer flavoprotein


free fatty acid


flavin mononucleotide


Rieske iron-sulfur protein


linoleic acid

MACP family

mitochondrial anion carrier protein family




nicotinamide adenine dinucleotide and its reduced form


nicotinamide adenine dinucleotide


phosphate and its reduced form








cycle: tricarboxylic acid cycle


uncoupling protein


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Copyright information

© Springer Science+Business Media Dordrecht 2004

Authors and Affiliations

  • Francis E. Sluse
    • 1
  • Wieslawa Jarmuszkiewicz
    • 2
  1. 1.Laboratory of Bioenergetics, Centre of Oxygen Research and Development, Department of Life Sciences, Institute of Chemistry B6cUniversity of LiegeLiegeBelgium
  2. 2.Laboratory of Bioenergetics, Institute of Molecular Biology and BiotechnologyAdam Mickiewicz UniversityPoznanPoland

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