Agrobacterium-Mediated Transformation of Canola
Canola (Brassica napus L.) is an important oil crop, ranking third only to soybean and palm oil in global production. It is a member of the family Brassicacea (syn. Cruciferae). It is a winter or spring crop and is amenable to growth in cooler climates. Once considered a specialty crop for Canada, it is now a global crop. Many other countries including the USA, Australia and those in Europe also grow canola. However, Canada and the United States account for most of the canola crop. It is grown mostly in Western Canada and North Central United States. In the year 2002, in Canada alone, 9.6 million acres of canola was grown (1) and in the USA, 1.5 million acres was devoted to canola cultivation (2). The term ‘canola’ was adopted by Canada apparently as an acronym of the Canadian Oilseed Association in 1979. Although canola has been commonly also known as rapeseed or oilseed rape, in the strict sense canola oil is defined as an oil that must contain less than 2% erucic acid, and the solid component of the seed must contain less than 30 μM of any one or a mixture of 3-butenyl glucosinolate, 4-pentenyl glucosinolate, 2-hydroxy-3 butenyl glucosinolate, and 2-hydroxy-4-pentenyl glucosinolate per gram of air-dry, oil-free solid.
KeywordsSurfactant Kanamycin Stearate Grease Chitinase
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