Abstract
Restriction fragment length polymorphisms (RFLPs) were used to assess genetic diversity of mitochondrial DNA (mtDNA) among standard isolates of seven lineages of Fusarium graminearum. The mtDNA patterns within each lineage were very similar (>89%), whereas significant differences were observed between the isolates belonging to different lineages, with the exception of lineages 1 and 4 where strong similarity was found between the RFLPs. Analysis of different band patterns resulted in characteristic HhaI and HaeIII bands that were suitable for identification of members of lineages 7, 6, 5, 3 and 2. Investigation of lineage distribution of 144 European isolates revealed that 142 belong to lineage 7. These data, therefore, confirmed the hypothesis that members of lineage 7 are predominant in Europe. Further analysis of isolates belonging to lineage 7 resulted in five haplotypes. These haplotypes have arisen as different combinations of three RFLP patterns for both HaeIII and HhaI restriction enzymes. Two isolates from Hungary, however, shared the same mtDNA RFLP profiles with a standard isolate of lineage 3, indicating that members of lineage 3, at a lower frequency, may also occur in Europe.
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References
Avise JC (1989) Gene trees and organismal histories: A phylogenetic approach to population biology. Evolution 43: 1192–1208.
Bottalico A (1998) Fusarium diseases of cereals: Species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology 80: 85–103.
Brasier CM (2000) The rise of hybrid fungi. Nature 405: 134–135.
Brown WN, George Jr. M and Wilson AC (1979) Rapid evolution of animal mitochondria] DNA. Proceedings of the National Academy of Sciences USA 76: 1967–1971.
Chelkowski J, Bateman GL and Mirocha CHJ (1999) Identification of toxigenic Fusarium species using PCR assays. Journal of Phytopathology 147: 307–311.
Chen L-F, Bai GH and Desjardins AE (2000) Recent advances in wheat head scab research in China. In: Proceedings of International Symposium on Wheat Improvement for Scab Resistance. (pp 258–273 ) Jiangsu, China.
Gale LR, Chen L-F, Hernick CA, Takamura K and Kistler HC (2002) Population analysis of Fusarium graminearum from wheat fields in Eastern China. Phytopathology 92: 1315–1322.
Lâday M and Szécsi A (2001) Distinct electrophoretic isozyme profiles of Fusarium graminearum and closely related species. Systematic and Applied Microbiology 24: 67–75.
Leach J, Finkelstein DB and Rambosek JA (1986) Rapid miniprep of DNA from filamentous fungi. Fungal Genetic Newsletters 33: 32–33.
McMullen MP, Jones R and Gallenberg D (1997) Scab of wheat and barley: A re-emerging disease of devastating impact. Plant Disease 81: 1340–1348.
Nei M and Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76: 5269–5273.
Nelson PE, Toussoun TA and Marasas WFO (1983) Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park, PA, USA.
Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW and Joyce D (1998) Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 53: 17–37.
Niessen ML and Vogel RF (1997) Specific identification of Fusarium graminearum by PCR with gaoA targeted primers. Systematic and Applied Microbiology 20: 111–113.
O’Donnell K, Kistler HC, Tacke BK and Casper HH (2000) Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences USA 97: 7905–7910.
Ouellet T and Seifert KA (1993) Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification. Phytopathology 83: 1003–1007.
Schilling AG, Möller EM and Geiger HH (1996) Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum, and F. avenaceum. Phytopathology 86: 515–522.
Snijders CHA (1990) Fusarium head blight and mycotoxin contamination of wheat, a review. Netherlands Journal of Plant Pathology 96: 187–198.
Yoder WT and Christianson LM (1998) Species-specific primers resolve members of Fusarium section Fusarium. Taxonomic status of the edible `Quorn’ fungus reevaluated. Fungal Genetics and Biology 23: 68–80.
Windels CE, Mirocha CJ, Abbas HK and Xie W (1989) Perithecium production in Fusarium graminearum populations and lack of correlation with zearalenone production. Mycologia 81: 272–277.
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Láday, M., Juhász, Á., Mulè, G., Moretti, A., Szécsi, Á., Logrieco, A. (2004). Mitochondrial DNA diversity and lineage determination of European isolates of Fusarium graminearum (Gibberella zeae). In: Mulè, G., Bailey, J.A., Cooke, B.M., Logrieco, A. (eds) Molecular Diversity and PCR-detection of Toxigenic Fusarium Species and Ochratoxigenic Fungi. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2285-2_9
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DOI: https://doi.org/10.1007/978-1-4020-2285-2_9
Publisher Name: Springer, Dordrecht
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