Abstract
A cDNA-AFLP approach was developed to identify differentially regulated genes in mycotoxin-producing and non-producing growth stages of Fusarium proliferatum (Gibberella fujikuori Mating Population-D). Using 160 PCR-primer combinations, a number of fragments which showed strikingly different intensities depending on growth stage were cloned, subjected to Northern analysis and sequenced. Two fragments, amplified from genes up-regulated during spore germination gave significant sequence homology to an amino acid transporter gene from Neurospora crassa and a GAL4-like transcriptional activator, respectively. Among the cDNAs derived from late growth stage transcripts, fragments of genes involved in polyol metabolism and cell cycle regulation, respectively were identified. Additional cDNAs (without significant homologies to known sequences) were also isolated and these clearly differentiated between mycotoxinproducing and non-producing growth stages of the fungus.
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Jeney, A., Béki, E., Mulè, G., Hornok, L. (2004). Identification of growth stage specific transcript profiles in Fusarium proliferatum (Gibberella fujikuroi, mating population D) by cDNA-AFLP analysis. In: Mulè, G., Bailey, J.A., Cooke, B.M., Logrieco, A. (eds) Molecular Diversity and PCR-detection of Toxigenic Fusarium Species and Ochratoxigenic Fungi. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2285-2_16
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DOI: https://doi.org/10.1007/978-1-4020-2285-2_16
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