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Interfertility of two mating populations in the Gibberella fujikuroi species complex

  • John F. Leslie
  • Kurt A. Zeller
  • Melissa Wohler
  • Brett A. Summerell

Abstract

Gibberella fujikuroi and Gibberella intermedia (mating populations ‘C’ and ‘D’ of the G. fujikuroi species complex) can be distinguished by differences in the spectrum of mycotoxins produced, the lack of sexual cross-fertility and diagnostic differences in their DNA sequences. Some isolates from these two biological species, however, can interbreed and complete meiosis to produce viable progeny. Analysis of marker segregation amongst such hybrid progeny can be used to estimate the degree of genomic rearrangement and genetic incompatibility that has accumulated since these sibling species diverged. Recombinant progeny were isolated from crosses of the standard tester strains for these two species and from crosses between these standard testers and a field isolate (KSU X-10626) that was cross-fertile with tester strains of both species. Progeny in all of the crosses segregated for amplified fragment length polymorphisms (AFLPs). Segregation of AFLP loci deviated from 1:1 for two thirds of the loci amongst the progeny of the cross between the ‘C’ and ‘D’ mating population tester strains, but <20% of the polymorphic loci in the cross of either tester with KSU X-10626 showed such distortion. It was concluded that G. intermedia and G. fujikuroi are sufficiently interfertile to belong to the same biological species, but that changing the nomenclature to reflect this interfertility requires more evidence for the natural occurrence of a continuum in fertility than is presently available.

Key words

AFLP biological species fumonisin Fusarium fujikuroi Fusarium proliferatum Gibberella intermedia interspecific hybrids Konza prairie mating type sexual crosses 

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References

  1. Britz H, Coutinho TA, Wingfield MJ, Marasas WFO, Gordon TR and Leslie JF (1999) Fusarium subglutinans f. sp. pini represents a distinct mating population in the Gibberella fujikuroi species complex. Applied and Environmental Microbiology 65: 1198–1201.Google Scholar
  2. Bullock S, Willetts HJ and Ashford AE (1980) The structure and histochemistry of sclerotia of Sclerotinia minor Jagger 1. Light and electron microscope studies on sclerotial development. Protoplasma 104: 315–331.Google Scholar
  3. Correll JC, Klittich CJR and Leslie JF (1987) Nitrate non-utilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77: 1640–1646.CrossRefGoogle Scholar
  4. Desjardins AE, Plattner RD and Nelson PE (1997) Production of fumonisin B1 and moniliformin by Gibberella fujikuroi from rice from various geographic areas. Applied and Environmental Microbiology 63: 1838–1842.PubMedGoogle Scholar
  5. Ellis JJ (1988a) Section Liseola of Fusarium. Mycologia 80: 255–258.CrossRefGoogle Scholar
  6. Ellis JJ (1988b) Lesser known species in section Liseola of Fusarium and near relatives in the Fusarium oxysporum group. Mycologia 80: 734–738.CrossRefGoogle Scholar
  7. Ellis JJ (1989) An alignment of toxigenic Gibberella strains having anamorphs in section Liseola of Fusarium. Mycologia 81: 307–311.CrossRefGoogle Scholar
  8. Feder N and O’Brien TP (1968) Plant microtechnique: Some principles and new methods. American Journal of Botany 55: 123–142.CrossRefGoogle Scholar
  9. Hsieh WH, Smith SN and Snyder WC (1977) Mating groups in Fusarium monilfforme. Phytopathology 67: 1041–1043.CrossRefGoogle Scholar
  10. Huss MJ, Campbell CL, Jennings DB and Leslie JF (1996) Isozyme variation among biological species in the Gibberella fujikuroi species complex (Fusarium section Liseola). Applied and Environmental Microbiology 62: 3750–3756.PubMedGoogle Scholar
  11. Jurgenson JE, Bowden RL, Zeller KA, Leslie JF, Alexander NJ and Plattner RD (2002) A genetic map of Gibberella zeae (Fusarium graminearum). Genetics 160: 1452–1460.Google Scholar
  12. Kerényi Z, Zeller KA, Hornok L and Leslie JF (1999) Standardization of mating-type terminology in the Gibberella fujikuroi species complex. Applied and Environmental Microbiology 65: 4071–4076.PubMedGoogle Scholar
  13. Klittich CJR and Leslie JF (1988) Nitrate reduction mutants of Fusarium monilfforme (Gibberella fujikuroi). Genetics 118: 417–423.PubMedGoogle Scholar
  14. Kuhlman EG (1982) Varieties of Gibberella fujikuroi with anamorphs in Fusarium section Liseola. Mycologia 74: 759–768.CrossRefGoogle Scholar
  15. Leslie JF (1995) Gibberella fujikuroi: Available populations and variable traits. Canadian Journal of Botany 73(Suppl. 1): S282–S291.Google Scholar
  16. Leslie JF and Klein KK (1996) Female fertility and mating-type effects on effective population size and evolution in filamentous fungi. Genetics 144: 557–567.PubMedGoogle Scholar
  17. Leslie JF, Pearson CAS, Nelson PE and Toussoun TA (1990) Fusarium species from corn, sorghum, and soybean fields in the central and eastern United States. Phytopathology 80: 343–350.Google Scholar
  18. Leslie JF, Zeller KA, Logrieco A, Mulè G, Moretti A and Ritieni A (2004) Species diversity and toxin production by strains in the Gibberella fujikuroi species complex isolated from native prairie grasses in Kansas. Applied and Environmental Microbiology 70 (4).Google Scholar
  19. Leslie JF, Zeller KA and Summerell BA (2001) Icebergs and species in populations of Fusarium. Physiological and Molecular Plant Pathology 59: 107–117.CrossRefGoogle Scholar
  20. Marasas WFO, Rheeder JP, Lamprecht SC, Zeller KA and Leslie JF (2001) Fusarium andiyazi sp. nov., a new species from sorghum. Mycologia 93: 1203–1210.Google Scholar
  21. Moretti A, Logrieco A, Bottalico A, Ritieni A, Fogliano V and Randazzo G (1996) Diversity in beauvericin and fusaproliferin production by different populations of Gibberella fujikuroi (Fusarium section Liseola). Sydowia 48: 44–56.Google Scholar
  22. Nirenberg HI and O’Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90: 434–458.CrossRefGoogle Scholar
  23. O’Donnell K, Cigelnik E and Nirenberg HI. 1998 Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465–493.CrossRefGoogle Scholar
  24. Perkins DD (1994a) Deviations from 1:1 and numbers of progeny necessary for establishing linkage. Fungal Genetics Newsletter 41: 69–70.Google Scholar
  25. Perkins DD (1994b) How should the interfertility of interspecies crosses be designated? Mycologia 86: 758–761.CrossRefGoogle Scholar
  26. Proctor RH, Desjardins AE, Plattner RD and Hohn TM (1999) A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genetics and Biology 27: 100–112.PubMedCrossRefGoogle Scholar
  27. Puhalla JE and Spieth PT (1985) A comparison of heterokaryosis and vegetative compatibility among varieties of Gibber-ella fujikuroi (Fusarium moniliforme). Experimental Mycology 9: 39–47.CrossRefGoogle Scholar
  28. Rheeder JP, Marasas WFO and Vismer HF (2002) Production of fumonisin analogs by Fusarium species. Applied and Environmental Microbiology 68: 2101–2105.PubMedCrossRefGoogle Scholar
  29. Samuels GJ, Nirenberg HI and Seifert KA (2001) Perithecial species of Fusarium. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL and Burgess LW (eds) Fusarium: Paul E. Nelson Memorial Symposium (pp 1–14 ) APS Press, St. Paul, MN, USA.Google Scholar
  30. Steenkamp ET, Wingfield BD, Coutinho TA, Wingfield MJ and Marasas WFO (1999) Differentiation of Fusarium subglutinans f. sp. pini by histone gene sequence data. Applied and Environmental Microbiology 65: 34013406.Google Scholar
  31. Steenkamp ET, Wingfield BD, Coutinho TA, Zeller KA, Wingfield MJ, Marasas WFO and Leslie JF (2000) PCRbased identification of MAT-1 and MAT-2 in the Gibberella fujikuroi species complex. Applied and Environmental Microbiology 66: 4378–4382.PubMedCrossRefGoogle Scholar
  32. Summerell BA, Salleh B and Leslie JF (2003) A utilitarian approach to Fusarium identification. Plant Disease 87: 117128.Google Scholar
  33. Tudzynski B (1999) Biosynthesis of gibberellins in Gibberella fujikuroi: Biomolecular aspects. Applied Microbiology and Biotechnology 52: 298–310.PubMedCrossRefGoogle Scholar
  34. Vos P, Hogers R, Sleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M and Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.Google Scholar
  35. Zeller KA, Jurgenson JE, El-Assiuty EM and Leslie JF (2000) Isozyme and amplified fragment length polymorphisms (AFLPs) from Cephalosporium maydis in Egypt. Phytoparasitica 28: 121–130.CrossRefGoogle Scholar
  36. Zeller KA, Summerell BA, Bullock S and Leslie JF (2003) Gibberella konza (Fusarium konzum) sp. nov., a new biological species within the Gibberella fujikuroi species complex from prairie grass. Mycologia 95: 943–954.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2004

Authors and Affiliations

  • John F. Leslie
    • 1
  • Kurt A. Zeller
    • 1
  • Melissa Wohler
    • 1
  • Brett A. Summerell
    • 2
  1. 1.Department of Plant Pathology, Throckmorton Plant Sciences CenterKansas State UniversityManhattanUSA
  2. 2.Royal Botanic Gardens and Domain TrustSydneyAustralia

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