State of the Art and Prospects of Macromolecular X-Ray Crystallography at High Hydrostatic Pressure
X-ray crystallography is by far the most important method of structural biology as it can provide a detailed picture at atomic or near-atomic resolution of the global structure of biological macromolecules such as proteins, nucleic acids and macromolecular assemblies of nearly arbitrary size and complexity. The resolution of the structure of ribosome, which is the most complex particle without internal symmetry ever solved by X-ray crystallography, is a flagship of modern crystallography. The interest of crystallographic results extends also to the pharmaceutical industry. An increasing number of new or potential drugs are based on the knowledge of the 3D structure of a particular protein and systematic studies of protein-inhibitor complexes. Further, a new stage of crystallography is well under way, in the frame of the structural genomics initiatives where all steps are modified or optimised in view of high throughput structure solution. In this respect, craftsman work has evolved towards high tech industrial processes.
KeywordsBragg Reflection Diffuse Scattering Diamond Anvil Cell Synchrotron Radiation Source Detective Quantum Efficiency
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