Some Useful Hints and Recipes for the Preparation and Examination of Stained Histological Sections

  • N. A. Ratcliffe


Since most mammalian organs and tissues cannot be examined in situ and are too thick to allow sufficient light to pass through them for microscopic examination, they must first be removed from the animal, cut into thin sections and then stained before their detailed structure can be examined under the compound light microscope. The following few pages contain a very brief outline of tissue preparation for the microscope and include some useful formulae and schedules which will hopefully assist the student in his laboratory work. Useful additional references for the more advanced student are also included.


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  1. Elias, R. G. (1979) Personal communication.Google Scholar
  2. Gomori, G. (1941). Am. J. Pathol., 17, 395–406.Google Scholar
  3. Humason, G. L. (1972). Animal Tissue Techniques, 3rd edn, W. H. Freeman, San Francisco (now available as 4th edn, 1978).Google Scholar
  4. McClung, C. E. (1937). Handbook of Microscopical Technique, 2nd edn, Oxford University Press, London.Google Scholar
  5. Pantin, C. F. A. (1964). Notes on Microscopical Technique for Zoologists, Cambridge University Press, Cambridge.Google Scholar
  6. Smith, A. and Bruton, J. (1978). A Colour Atlas of Histological Staining Techiques, Wolfe Medical Publications, London.Google Scholar
  7. Steedman, H. F. (1960). Section Cutting in Microscopy, Blackwell, Oxford.Google Scholar
  8. Wallington, E. A. (1972). Histological Methods for Bone, Butterworths, London.Google Scholar

Copyright information

© Macmillan Publishers Limited 1982

Authors and Affiliations

  • N. A. Ratcliffe
    • 1
  1. 1.Department of ZoologyUniversity College of SwanseaUK

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